Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress
Naturally available plant growth-promoting rhizobacteria (PGPR) have 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes, and are capable of processing the plant-borne ACC by converting it into α-ketobutyrate and ammonia. Thus, the PGPRs help in the depletion of ethylene levels, and enhanc...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/1/46 |
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| author | Aruna Kumari Andy Vishnu D. Rajput Marina Burachevskaya Vinod Singh Gour |
| author_facet | Aruna Kumari Andy Vishnu D. Rajput Marina Burachevskaya Vinod Singh Gour |
| author_sort | Aruna Kumari Andy |
| collection | DOAJ |
| description | Naturally available plant growth-promoting rhizobacteria (PGPR) have 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes, and are capable of processing the plant-borne ACC by converting it into α-ketobutyrate and ammonia. Thus, the PGPRs help in the depletion of ethylene levels, and enhance abiotic stress tolerance in plants. In the present study, two rhizobacterial strains, i.e., <i>Bacillus cereus</i> and <i>B. haynesii,</i> isolated from <i>Vigna mungo</i> and <i>Phaseolus vulgaris,</i> were used. These strains were taxonomically identified by 16S rDNA sequencing as <i>B. cereus</i> and <i>B. haynesii,</i> with NCBI accession numbers LC514122 and LC 514123, respectively. The phylogeny of these strains has also been worked out based on homology, with data available on NCBI GenBank. The strains were screened for their plant growth-promoting traits, and quantified in the same way. The enzymatic activity and molecular weight of the ACC deaminase obtained from both bacterial strains have also been determined. An in vitro drought tolerance study was done by using PEG 6000. These bacterial strains exhibited higher ACC deaminase activity (~5 to 6 µmol/mL), exopolysaccharide yield (15 to 18 mg/10 mL protein), and indole acetic acid (27–32 µg/mL). These characteristics indicate that the bacterial strains under present study may be helpful in enhancing the drought tolerance of the crops with enhanced yield. <i>Bacillus cereus</i> has been found to be a tolerant strain to As, Ba, and Ni, based on the plate assay method, and so it has the potential to be used as biofertilizer in fields affected by these metals. |
| first_indexed | 2024-03-09T12:30:38Z |
| format | Article |
| id | doaj.art-532fb16f8b044f83b41353e00fe185b1 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T12:30:38Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-532fb16f8b044f83b41353e00fe185b12023-11-30T22:30:08ZengMDPI AGHorticulturae2311-75242023-01-01914610.3390/horticulturae9010046Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal StressAruna Kumari Andy0Vishnu D. Rajput1Marina Burachevskaya2Vinod Singh Gour3Directorate of Research, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 211007, IndiaAcademy of Biology and Biotechnology, Southern Federal University, 344006 Rostov-on-Don, RussiaSoil Chemistry and Ecology Laboratory, Faculty of Natural Sciences, Tula State Lev Tolstoy Pedagogical University, Lenin Avenue, 125, 300026 Tula, RussiaAmity Institute of Biotechnology, Amity University Rajasthan, Jaipur 303002, IndiaNaturally available plant growth-promoting rhizobacteria (PGPR) have 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzymes, and are capable of processing the plant-borne ACC by converting it into α-ketobutyrate and ammonia. Thus, the PGPRs help in the depletion of ethylene levels, and enhance abiotic stress tolerance in plants. In the present study, two rhizobacterial strains, i.e., <i>Bacillus cereus</i> and <i>B. haynesii,</i> isolated from <i>Vigna mungo</i> and <i>Phaseolus vulgaris,</i> were used. These strains were taxonomically identified by 16S rDNA sequencing as <i>B. cereus</i> and <i>B. haynesii,</i> with NCBI accession numbers LC514122 and LC 514123, respectively. The phylogeny of these strains has also been worked out based on homology, with data available on NCBI GenBank. The strains were screened for their plant growth-promoting traits, and quantified in the same way. The enzymatic activity and molecular weight of the ACC deaminase obtained from both bacterial strains have also been determined. An in vitro drought tolerance study was done by using PEG 6000. These bacterial strains exhibited higher ACC deaminase activity (~5 to 6 µmol/mL), exopolysaccharide yield (15 to 18 mg/10 mL protein), and indole acetic acid (27–32 µg/mL). These characteristics indicate that the bacterial strains under present study may be helpful in enhancing the drought tolerance of the crops with enhanced yield. <i>Bacillus cereus</i> has been found to be a tolerant strain to As, Ba, and Ni, based on the plate assay method, and so it has the potential to be used as biofertilizer in fields affected by these metals.https://www.mdpi.com/2311-7524/9/1/46PGPR characterizationACC deaminaseexopolysaccharidesmolecular diversity |
| spellingShingle | Aruna Kumari Andy Vishnu D. Rajput Marina Burachevskaya Vinod Singh Gour Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress Horticulturae PGPR characterization ACC deaminase exopolysaccharides molecular diversity |
| title | Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress |
| title_full | Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress |
| title_fullStr | Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress |
| title_full_unstemmed | Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress |
| title_short | Exploring the Identity and Properties of Two <i>Bacilli</i> Strains and their Potential to Alleviate Drought and Heavy Metal Stress |
| title_sort | exploring the identity and properties of two i bacilli i strains and their potential to alleviate drought and heavy metal stress |
| topic | PGPR characterization ACC deaminase exopolysaccharides molecular diversity |
| url | https://www.mdpi.com/2311-7524/9/1/46 |
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