Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production.
Plexins are a family of genes (A,B,C, and D) that are expressed in many organ systems. Plexins expressed in the immune system have been implicated in cell movement and cell-cell interaction during the course of an immune response. In this study, the expression pattern of Plexin-B2 and Plexin-D1 in d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3419716?pdf=render |
| _version_ | 1819127993976487936 |
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| author | Eda K Holl Kelly E Roney Irving C Allen Erin Steinbach Janelle C Arthur Adam Buntzman Scott Plevy Jeffrey Frelinger Jenny P-Y Ting |
| author_facet | Eda K Holl Kelly E Roney Irving C Allen Erin Steinbach Janelle C Arthur Adam Buntzman Scott Plevy Jeffrey Frelinger Jenny P-Y Ting |
| author_sort | Eda K Holl |
| collection | DOAJ |
| description | Plexins are a family of genes (A,B,C, and D) that are expressed in many organ systems. Plexins expressed in the immune system have been implicated in cell movement and cell-cell interaction during the course of an immune response. In this study, the expression pattern of Plexin-B2 and Plexin-D1 in dendritic cells (DCs), which are central in immune activation, was investigated. Plexin-B2 and Plexin-D1 are reciprocally expressed in myeloid and plasmacytoid DC populations. Plasmacytoid DCs have high Plexin-B2 but low Plexin-D1, while the opposite is true of myeloid DCs. Expression of Plexin-B2 and Plexin-D1 is modulated upon activation of DCs by TLR ligands, TNFα, and anti-CD40, again in a reciprocal fashion. Semaphorin3E, a ligand for Plexin-D1 and Plexin-B2, is expressed by T cells, and interestingly, is dramatically higher on Th2 cells and on DCs. The expression of Plexins and their ligands on DCs and T cells suggest functional relevance. To explore this, we utilized chimeric mice lacking Plxnb2 or Plxnd1. Absence of Plexin-B2 and Plexin-D1 on DCs did not affect the ability of these cells to upregulate costimulatory molecules or the ability of these cells to activate antigen specific T cells. Additionally, Plexin-B2 and Plexin-D1 were dispensable for chemokine-directed in-vitro migration of DCs towards key DC chemokines, CXCL12 and CCL19. However, the absence of either Plexin-B2 or Plexin-D1 on DCs leads to constitutive expression of IL-12/IL-23p40. This is the first report to show an association between Plexin-B2 and Plexin-D1 with the negative regulation of IL-12/IL-23p40 in DCs. This work also shows the presence of Plexin-B2 and Plexin-D1 on mouse DC subpopulations, and indicates that these two proteins play a role in IL-12/IL-23p40 production that is likely to impact the immune response. |
| first_indexed | 2024-12-22T08:20:46Z |
| format | Article |
| id | doaj.art-5334cddaa0cb448bb54df1cad53bbb5d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:20:46Z |
| publishDate | 2012-01-01 |
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| spelling | doaj.art-5334cddaa0cb448bb54df1cad53bbb5d2022-12-21T18:32:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4333310.1371/journal.pone.0043333Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production.Eda K HollKelly E RoneyIrving C AllenErin SteinbachJanelle C ArthurAdam BuntzmanScott PlevyJeffrey FrelingerJenny P-Y TingPlexins are a family of genes (A,B,C, and D) that are expressed in many organ systems. Plexins expressed in the immune system have been implicated in cell movement and cell-cell interaction during the course of an immune response. In this study, the expression pattern of Plexin-B2 and Plexin-D1 in dendritic cells (DCs), which are central in immune activation, was investigated. Plexin-B2 and Plexin-D1 are reciprocally expressed in myeloid and plasmacytoid DC populations. Plasmacytoid DCs have high Plexin-B2 but low Plexin-D1, while the opposite is true of myeloid DCs. Expression of Plexin-B2 and Plexin-D1 is modulated upon activation of DCs by TLR ligands, TNFα, and anti-CD40, again in a reciprocal fashion. Semaphorin3E, a ligand for Plexin-D1 and Plexin-B2, is expressed by T cells, and interestingly, is dramatically higher on Th2 cells and on DCs. The expression of Plexins and their ligands on DCs and T cells suggest functional relevance. To explore this, we utilized chimeric mice lacking Plxnb2 or Plxnd1. Absence of Plexin-B2 and Plexin-D1 on DCs did not affect the ability of these cells to upregulate costimulatory molecules or the ability of these cells to activate antigen specific T cells. Additionally, Plexin-B2 and Plexin-D1 were dispensable for chemokine-directed in-vitro migration of DCs towards key DC chemokines, CXCL12 and CCL19. However, the absence of either Plexin-B2 or Plexin-D1 on DCs leads to constitutive expression of IL-12/IL-23p40. This is the first report to show an association between Plexin-B2 and Plexin-D1 with the negative regulation of IL-12/IL-23p40 in DCs. This work also shows the presence of Plexin-B2 and Plexin-D1 on mouse DC subpopulations, and indicates that these two proteins play a role in IL-12/IL-23p40 production that is likely to impact the immune response.http://europepmc.org/articles/PMC3419716?pdf=render |
| spellingShingle | Eda K Holl Kelly E Roney Irving C Allen Erin Steinbach Janelle C Arthur Adam Buntzman Scott Plevy Jeffrey Frelinger Jenny P-Y Ting Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. PLoS ONE |
| title | Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. |
| title_full | Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. |
| title_fullStr | Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. |
| title_full_unstemmed | Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. |
| title_short | Plexin-B2 and Plexin-D1 in dendritic cells: expression and IL-12/IL-23p40 production. |
| title_sort | plexin b2 and plexin d1 in dendritic cells expression and il 12 il 23p40 production |
| url | http://europepmc.org/articles/PMC3419716?pdf=render |
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