In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy
Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity which represents ideal environment for metallic ion release and biodegradation. The released metallic ions from dental materials can cause local and/or systemic adverse effects in the human...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2015-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2015/0352-51391500070D.pdf |
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| author | Dimić Ivana Cvijović-Alagić Ivana Obradović Nataša Petrović Jelena Putić Slaviša Rakin Marko Bugarski Branko |
| author_facet | Dimić Ivana Cvijović-Alagić Ivana Obradović Nataša Petrović Jelena Putić Slaviša Rakin Marko Bugarski Branko |
| author_sort | Dimić Ivana |
| collection | DOAJ |
| description | Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive
conditions in the oral cavity which represents ideal environment for metallic
ion release and biodegradation. The released metallic ions from dental
materials can cause local and/or systemic adverse effects in the human body.
Therefore, the dental materials are required to possess appropriate
mechanical, physical, chemical and biological properties. The
biocompatibility of metallic materials is very important for dental
applications. Accordingly, the aim of this study was to examine metallic ion
release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of
biocompatibility evaluation. Determination of human (MRC-5) and animal (L929)
fibroblast cells viability was conducted using three in vitro test methods:
colorimetric methyl-thiazol-tetrazolium (MTT) test, dye exclusion test (DET)
and agar diffusion test (ADT). Furthermore, the cells morphology and growth
were analyzed using scanning electron microscopy (SEM). The obtained results
indicated that Co-30Cr-5Mo alloy did not release harmful elements in high
concentrations that could cause detrimental effects on human and animal
fibroblasts under the given experimental conditions. Moreover, the fibroblast
cells showed good adhesion on the Co-30Cr-5Mo alloy surface. Therefore, it
can be concluded that Co-30Cr-5Mo alloy is biocompatible material which can
be safely used in dentistry. [Projekat Ministarstva nauke Republike Srbije,
br. III 46010 i br. ON 174004] |
| first_indexed | 2024-12-22T15:46:07Z |
| format | Article |
| id | doaj.art-5335fd02a92a42bf8a4dc45e71656ca8 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-22T15:46:07Z |
| publishDate | 2015-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-5335fd02a92a42bf8a4dc45e71656ca82022-12-21T18:21:00ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212015-01-0180121541155210.2298/JSC150505070M0352-51391500070DIn vitro biocompatibility assessment of Co-Cr-Mo dental cast alloyDimić Ivana0Cvijović-Alagić Ivana1Obradović Nataša2Petrović Jelena3Putić Slaviša4Rakin Marko5Bugarski Branko6Faculty of Technology and Metallurgy, Innovation Centre, BelgradeInstitute of Nuclear Sciences "Vinča", BelgradeFaculty of Technology and Metallurgy, Innovation Centre, BelgradeFaculty of Technology and Metallurgy, BelgradeFaculty of Technology and Metallurgy, BelgradeFaculty of Technology and Metallurgy, BelgradeFaculty of Technology and Metallurgy, BelgradeMetallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity which represents ideal environment for metallic ion release and biodegradation. The released metallic ions from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, the dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of human (MRC-5) and animal (L929) fibroblast cells viability was conducted using three in vitro test methods: colorimetric methyl-thiazol-tetrazolium (MTT) test, dye exclusion test (DET) and agar diffusion test (ADT). Furthermore, the cells morphology and growth were analyzed using scanning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in high concentrations that could cause detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the Co-30Cr-5Mo alloy surface. Therefore, it can be concluded that Co-30Cr-5Mo alloy is biocompatible material which can be safely used in dentistry. [Projekat Ministarstva nauke Republike Srbije, br. III 46010 i br. ON 174004]http://www.doiserbia.nb.rs/img/doi/0352-5139/2015/0352-51391500070D.pdfCo-based alloybiomaterialscytotoxicityfibroblasts |
| spellingShingle | Dimić Ivana Cvijović-Alagić Ivana Obradović Nataša Petrović Jelena Putić Slaviša Rakin Marko Bugarski Branko In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy Journal of the Serbian Chemical Society Co-based alloy biomaterials cytotoxicity fibroblasts |
| title | In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy |
| title_full | In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy |
| title_fullStr | In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy |
| title_full_unstemmed | In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy |
| title_short | In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy |
| title_sort | in vitro biocompatibility assessment of co cr mo dental cast alloy |
| topic | Co-based alloy biomaterials cytotoxicity fibroblasts |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2015/0352-51391500070D.pdf |
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