The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.
Control over gene expression is exerted, in multiple stages of spermatogenesis, at the post-transcriptional level by RNA binding proteins (RBPs). We identify here an essential role in mammalian spermatogenesis and male fertility for 'RNA binding protein 46' (RBM46). A highly evolutionarily...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-09-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010416 |
| _version_ | 1797823697208213504 |
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| author | Natoya J Peart Taylor A Johnson Sungkyoung Lee Matthew J Sears Fang Yang Mathieu Quesnel-Vallières Huijuan Feng Yocelyn Recinos Yoseph Barash Chaolin Zhang Brian P Hermann P Jeremy Wang Christopher B Geyer Russ P Carstens |
| author_facet | Natoya J Peart Taylor A Johnson Sungkyoung Lee Matthew J Sears Fang Yang Mathieu Quesnel-Vallières Huijuan Feng Yocelyn Recinos Yoseph Barash Chaolin Zhang Brian P Hermann P Jeremy Wang Christopher B Geyer Russ P Carstens |
| author_sort | Natoya J Peart |
| collection | DOAJ |
| description | Control over gene expression is exerted, in multiple stages of spermatogenesis, at the post-transcriptional level by RNA binding proteins (RBPs). We identify here an essential role in mammalian spermatogenesis and male fertility for 'RNA binding protein 46' (RBM46). A highly evolutionarily conserved gene, Rbm46 is also essential for fertility in both flies and fish. We found Rbm46 expression was restricted to the mouse germline, detectable in males in the cytoplasm of premeiotic spermatogonia and meiotic spermatocytes. To define its requirement for spermatogenesis, we generated Rbm46 knockout (KO, Rbm46-/-) mice; although male Rbm46-/- mice were viable and appeared grossly normal, they were infertile. Testes from adult Rbm46-/- mice were small, with seminiferous tubules containing only Sertoli cells and few undifferentiated spermatogonia. Using genome-wide unbiased high throughput assays RNA-seq and 'enhanced crosslinking immunoprecipitation' coupled with RNA-seq (eCLIP-seq), we discovered RBM46 could bind, via a U-rich conserved consensus sequence, to a cohort of mRNAs encoding proteins required for completion of differentiation and subsequent meiotic initiation. In summary, our studies support an essential role for RBM46 in regulating target mRNAs during spermatogonia differentiation prior to the commitment to meiosis in mice. |
| first_indexed | 2024-03-13T10:26:45Z |
| format | Article |
| id | doaj.art-53361225d7ba4d6abbf2e4eaf7ec0fd2 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-03-13T10:26:45Z |
| publishDate | 2022-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-53361225d7ba4d6abbf2e4eaf7ec0fd22023-05-19T05:31:05ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-09-01189e101041610.1371/journal.pgen.1010416The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.Natoya J PeartTaylor A JohnsonSungkyoung LeeMatthew J SearsFang YangMathieu Quesnel-VallièresHuijuan FengYocelyn RecinosYoseph BarashChaolin ZhangBrian P HermannP Jeremy WangChristopher B GeyerRuss P CarstensControl over gene expression is exerted, in multiple stages of spermatogenesis, at the post-transcriptional level by RNA binding proteins (RBPs). We identify here an essential role in mammalian spermatogenesis and male fertility for 'RNA binding protein 46' (RBM46). A highly evolutionarily conserved gene, Rbm46 is also essential for fertility in both flies and fish. We found Rbm46 expression was restricted to the mouse germline, detectable in males in the cytoplasm of premeiotic spermatogonia and meiotic spermatocytes. To define its requirement for spermatogenesis, we generated Rbm46 knockout (KO, Rbm46-/-) mice; although male Rbm46-/- mice were viable and appeared grossly normal, they were infertile. Testes from adult Rbm46-/- mice were small, with seminiferous tubules containing only Sertoli cells and few undifferentiated spermatogonia. Using genome-wide unbiased high throughput assays RNA-seq and 'enhanced crosslinking immunoprecipitation' coupled with RNA-seq (eCLIP-seq), we discovered RBM46 could bind, via a U-rich conserved consensus sequence, to a cohort of mRNAs encoding proteins required for completion of differentiation and subsequent meiotic initiation. In summary, our studies support an essential role for RBM46 in regulating target mRNAs during spermatogonia differentiation prior to the commitment to meiosis in mice.https://doi.org/10.1371/journal.pgen.1010416 |
| spellingShingle | Natoya J Peart Taylor A Johnson Sungkyoung Lee Matthew J Sears Fang Yang Mathieu Quesnel-Vallières Huijuan Feng Yocelyn Recinos Yoseph Barash Chaolin Zhang Brian P Hermann P Jeremy Wang Christopher B Geyer Russ P Carstens The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. PLoS Genetics |
| title | The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. |
| title_full | The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. |
| title_fullStr | The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. |
| title_full_unstemmed | The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. |
| title_short | The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. |
| title_sort | germ cell specific rna binding protein rbm46 is essential for spermatogonial differentiation in mice |
| url | https://doi.org/10.1371/journal.pgen.1010416 |
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