Development of a Melting Curve-Based Triple Eva Green Real-Time PCR Assay for Simultaneous Detection of Three Shrimp Pathogens

Development of a Melting Curve-Based Triple Eva Green Real-Time PCR Assay for Simultaneous Detection of Three Shrimp Pathogens

Infections with <i>Enterocytozoon hepatopenaei</i> (EHP), infectious hypodermal and hematopoietic necrosis virus (IHHNV), and Decapod iridescent virus 1 (DIV1) pose significant challenges to the shrimp industry. Here, a melting curve-based triple real-time PCR assay based on the fluoresc...

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Bibliographic Details
Main Authors: Xuan Dong, Yujin Chen, Haoyu Lou, Guohao Wang, Chengyan Zhou, Liying Wang, Xuan Li, Jingfei Luo, Jie Huang
Format: Article
Language:English
Published: MDPI AG 2024-02-01
Series:Animals
Subjects:
aquaculture
shrimp pathogens
Eva Green
real-time PCR
detection
Online Access:https://www.mdpi.com/2076-2615/14/4/592

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