<i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue
<i>Streptococcus suis</i> is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different bio...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/366 |
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| author | Muriel Dresen Josephine Schenk Yenehiwot Berhanu Weldearegay Désirée Vötsch Wolfgang Baumgärtner Peter Valentin-Weigand Andreas Nerlich |
| author_facet | Muriel Dresen Josephine Schenk Yenehiwot Berhanu Weldearegay Désirée Vötsch Wolfgang Baumgärtner Peter Valentin-Weigand Andreas Nerlich |
| author_sort | Muriel Dresen |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE<sub>2</sub> levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE<sub>2</sub> production was detected following infection with two prevalent <i>S. suis</i> serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to <i>S. suis</i> infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with <i>S. suis</i>. |
| first_indexed | 2024-03-09T00:55:29Z |
| format | Article |
| id | doaj.art-533706f27ad74f0fa8e1298facf8e5c6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T00:55:29Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-533706f27ad74f0fa8e1298facf8e5c62023-12-11T16:54:32ZengMDPI AGMicroorganisms2076-26072021-02-019236610.3390/microorganisms9020366<i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung TissueMuriel Dresen0Josephine Schenk1Yenehiwot Berhanu Weldearegay2Désirée Vötsch3Wolfgang Baumgärtner4Peter Valentin-Weigand5Andreas Nerlich6Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Pathology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, GermanyInstitute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany<i>Streptococcus suis</i> is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE<sub>2</sub> levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE<sub>2</sub> production was detected following infection with two prevalent <i>S. suis</i> serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to <i>S. suis</i> infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with <i>S. suis</i>.https://www.mdpi.com/2076-2607/9/2/366<i>Streptococcus suis</i>COX-2cyclooxygenaseinflammationlung infectionprecision-cut lung slices |
| spellingShingle | Muriel Dresen Josephine Schenk Yenehiwot Berhanu Weldearegay Désirée Vötsch Wolfgang Baumgärtner Peter Valentin-Weigand Andreas Nerlich <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue Microorganisms <i>Streptococcus suis</i> COX-2 cyclooxygenase inflammation lung infection precision-cut lung slices |
| title | <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
| title_full | <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
| title_fullStr | <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
| title_full_unstemmed | <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
| title_short | <i>Streptococcus suis</i> Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
| title_sort | i streptococcus suis i induces expression of cyclooxygenase 2 in porcine lung tissue |
| topic | <i>Streptococcus suis</i> COX-2 cyclooxygenase inflammation lung infection precision-cut lung slices |
| url | https://www.mdpi.com/2076-2607/9/2/366 |
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