A novel system for stable, high-level expression from the T7 promoter
<p>Abstract</p> <p>Background</p> <p>The most widespread, efficient prokaryotic protein-producing system is one where the T7 phage polymerase recognizes the T7 phage promoter (T7 p/p system). Unfortunately, in this system, target protein expression gradually declines an...
Main Authors: | Kesik-Brodacka Malgorzata, Romanik Agnieszka, Mikiewicz-Sygula Diana, Plucienniczak Grazyna, Plucienniczak Andrzej |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2012-08-01
|
Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://www.microbialcellfactories.com/content/11/1/109 |
Similar Items
-
Developing Michigan Cancer Foundation 7 Cells with Stable Expression of E7 Gene of Human Papillomavirus Type 16
by: Faezeh Ghasemi, et al.
Published: (2016-01-01) -
Characterization of Context-Dependent Effects on Synthetic Promoters
by: Sebastian Köbbing, et al.
Published: (2020-06-01) -
T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation
by: Daria Krefft, et al.
Published: (2022-01-01) -
Gene Expression of miRNAs Let-7aAssociated with Diabetes in Iraqi Population
by: N Sabah Younus, et al.
Published: (2021-10-01) -
A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
by: Jing Ye, et al.
Published: (2022-05-01)