ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L
<i>Marchantia polymorpha</i> L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of <i>M. polymorpha</i> to high d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/7/1478 |
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| author | Nahuel Spinedi Romina Storb Elisabet Aranda Facundo Romani Maya Svriz Santiago A. Varela Javier E. Moreno Sebastian Fracchia Juan Cabrera Ramón Alberto Batista-García Inés Ponce de León J. Martín Scervino |
| author_facet | Nahuel Spinedi Romina Storb Elisabet Aranda Facundo Romani Maya Svriz Santiago A. Varela Javier E. Moreno Sebastian Fracchia Juan Cabrera Ramón Alberto Batista-García Inés Ponce de León J. Martín Scervino |
| author_sort | Nahuel Spinedi |
| collection | DOAJ |
| description | <i>Marchantia polymorpha</i> L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of <i>M. polymorpha</i> to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl <i>a</i> and 38.4% of Chl <i>b</i>, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (<i>Fv/Fm</i>). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that <i>M. polymorpha</i> tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that <i>M. polymorpha</i> under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus. |
| first_indexed | 2024-03-10T09:27:29Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T09:27:29Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-533ae63979cb493ab64d892d6e80797f2023-11-22T04:44:54ZengMDPI AGPlants2223-77472021-07-01107147810.3390/plants10071478ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> LNahuel Spinedi0Romina Storb1Elisabet Aranda2Facundo Romani3Maya Svriz4Santiago A. Varela5Javier E. Moreno6Sebastian Fracchia7Juan Cabrera8Ramón Alberto Batista-García9Inés Ponce de León10J. Martín Scervino11Instituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), CONICET-UNCo, SC Bariloche, Río Negro 8400, ArgentinaInstituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), CONICET-UNCo, SC Bariloche, Río Negro 8400, ArgentinaInstitute of Water Research, University of Granada, Ramón y Cajal, 4, Bldg. Fray Luís, 18071 Granada, SpainInstituto de Agrobiotecnología del Litoral, UNL-Conicet, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe 3000, ArgentinaInstituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural (IRNAD), Universidad Nacional de Río Negro, CONICET, SC Bariloche, Río Negro 8400, ArgentinaGrupo de Ecología Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA) EEA Bariloche, CC 277, Bariloche 8400, ArgentinaInstituto de Agrobiotecnología del Litoral, UNL-Conicet, Facultad de Bioquímica y Ciencias Biológicas, Santa Fe 3000, ArgentinaCentro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja (CRILAR), UNLAR, SEGEMAR, UNCa, CONICET, Entre Ríos y Mendoza, 530 Anillaco, La Rioja 5300, ArgentinaInstituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), CONICET-UNCo, SC Bariloche, Río Negro 8400, ArgentinaCentro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Ave Universidad 1001, Col. Chamilpa, CP 62209 Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, CP 11600 Montevideo, UruguayInstituto de Investigaciones en Biodiversidad y Medioambiente (INIBIOMA), CONICET-UNCo, SC Bariloche, Río Negro 8400, Argentina<i>Marchantia polymorpha</i> L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological response of <i>M. polymorpha</i> to high doses of anthracene analysing the antioxidant enzymes and their relationship with the photosynthetic processes, as well as their transcriptomic response. We found an anthracene dose-dependent response reducing plant biomass and associated to an alteration of the ultrastructure of a 23.6% of chloroplasts. Despite a reduction in total thallus-chlorophyll of 31.6% of Chl <i>a</i> and 38.4% of Chl <i>b</i>, this was not accompanied by a significant change in the net photosynthesis rate and maximum quantum efficiency (<i>Fv/Fm</i>). However, we found an increase in the activity of main ROS-detoxifying enzymes of 34.09% of peroxidase and 692% of ascorbate peroxidase, supported at transcriptional level with the upregulation of ROS-related detoxifying responses. Finally, we found that <i>M. polymorpha</i> tolerated anthracene-stress under the lowest concentration used and can suffer physiological alterations under higher concentrations tested related to the accumulation of anthracene within plant tissues. Our results show that <i>M. polymorpha</i> under PAH stress condition activated two complementary physiological responses including the activation of antioxidant mechanisms and the accumulation of the pollutant within plant tissues to mitigate the damage to the photosynthetic apparatus.https://www.mdpi.com/2223-7747/10/7/1478antioxidant enzymesperoxidasesliverwortpolycyclic aromatic hydrocarbonsanthracenephotosynthesis |
| spellingShingle | Nahuel Spinedi Romina Storb Elisabet Aranda Facundo Romani Maya Svriz Santiago A. Varela Javier E. Moreno Sebastian Fracchia Juan Cabrera Ramón Alberto Batista-García Inés Ponce de León J. Martín Scervino ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L Plants antioxidant enzymes peroxidases liverwort polycyclic aromatic hydrocarbons anthracene photosynthesis |
| title | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L |
| title_full | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L |
| title_fullStr | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L |
| title_full_unstemmed | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L |
| title_short | ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort <i>Marchantia polymorpha</i> L |
| title_sort | ros scavenging enzymes as an antioxidant response to high concentration of anthracene in the liverwort i marchantia polymorpha i l |
| topic | antioxidant enzymes peroxidases liverwort polycyclic aromatic hydrocarbons anthracene photosynthesis |
| url | https://www.mdpi.com/2223-7747/10/7/1478 |
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