Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells
Amaranthus plants, or spinach, are used extensively as a vegetable and are known to possess medicinal properties. Neuroinflammation and oxidative stress play a major role in the pathogenesis of many neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. Advanced glycation e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/9/17288 |
| _version_ | 1831738499794993152 |
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| author | Warisa Amornrit Rachana Santiyanont |
| author_facet | Warisa Amornrit Rachana Santiyanont |
| author_sort | Warisa Amornrit |
| collection | DOAJ |
| description | Amaranthus plants, or spinach, are used extensively as a vegetable and are known to possess medicinal properties. Neuroinflammation and oxidative stress play a major role in the pathogenesis of many neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. Advanced glycation end-products (AGEs) cause cell toxicity in the human neuronal cell line, SH-SY5Y, through an increase in oxidative stress, as shown by reducing cell viability and increasing cell toxicity in a dose-dependent manner. We found that preincubation of SH-SY5Y cells with either petroleum ether, dichloromethane or methanol extracts of A. lividus and A. tricolor dose-dependently attenuated the neuron toxicity caused by AGEs treatment. Moreover, the results showed that A. lividus and A. tricolor extracts significantly downregulated the gene expression of the pro-inflammatory cytokines, TNF-α, IL-1 and IL-6 genes in AGEs-induced cells. We concluded that A. lividus and A. tricolor extracts not only have a neuroprotective effect against AGEs toxicity, but also have anti-inflammatory activity by reducing pro-inflammatory cytokine gene expression. This suggests that Amaranthus may be useful for treating chronic inflammation associated with neurodegenerative disorders. |
| first_indexed | 2024-12-21T13:23:34Z |
| format | Article |
| id | doaj.art-533c1e1346e64f039b9c57ee33ca69ca |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T13:23:34Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-533c1e1346e64f039b9c57ee33ca69ca2022-12-21T19:02:32ZengMDPI AGMolecules1420-30492015-09-01209172881730810.3390/molecules200917288molecules200917288Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y CellsWarisa Amornrit0Rachana Santiyanont1Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandAmaranthus plants, or spinach, are used extensively as a vegetable and are known to possess medicinal properties. Neuroinflammation and oxidative stress play a major role in the pathogenesis of many neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. Advanced glycation end-products (AGEs) cause cell toxicity in the human neuronal cell line, SH-SY5Y, through an increase in oxidative stress, as shown by reducing cell viability and increasing cell toxicity in a dose-dependent manner. We found that preincubation of SH-SY5Y cells with either petroleum ether, dichloromethane or methanol extracts of A. lividus and A. tricolor dose-dependently attenuated the neuron toxicity caused by AGEs treatment. Moreover, the results showed that A. lividus and A. tricolor extracts significantly downregulated the gene expression of the pro-inflammatory cytokines, TNF-α, IL-1 and IL-6 genes in AGEs-induced cells. We concluded that A. lividus and A. tricolor extracts not only have a neuroprotective effect against AGEs toxicity, but also have anti-inflammatory activity by reducing pro-inflammatory cytokine gene expression. This suggests that Amaranthus may be useful for treating chronic inflammation associated with neurodegenerative disorders.http://www.mdpi.com/1420-3049/20/9/17288AGEsoxidative stressSH-SY5Y cellsAmaranthusproinflammatory cytokines |
| spellingShingle | Warisa Amornrit Rachana Santiyanont Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells Molecules AGEs oxidative stress SH-SY5Y cells Amaranthus proinflammatory cytokines |
| title | Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells |
| title_full | Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells |
| title_fullStr | Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells |
| title_full_unstemmed | Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells |
| title_short | Effect of Amaranthus on Advanced Glycation End-Products Induced Cytotoxicity and Proinflammatory Cytokine Gene Expression in SH-SY5Y Cells |
| title_sort | effect of amaranthus on advanced glycation end products induced cytotoxicity and proinflammatory cytokine gene expression in sh sy5y cells |
| topic | AGEs oxidative stress SH-SY5Y cells Amaranthus proinflammatory cytokines |
| url | http://www.mdpi.com/1420-3049/20/9/17288 |
| work_keys_str_mv | AT warisaamornrit effectofamaranthusonadvancedglycationendproductsinducedcytotoxicityandproinflammatorycytokinegeneexpressioninshsy5ycells AT rachanasantiyanont effectofamaranthusonadvancedglycationendproductsinducedcytotoxicityandproinflammatorycytokinegeneexpressioninshsy5ycells |