Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates
Background: There are only a few reports in the literature about translocation of coagulase-negative staphylococci (CoNS) as a primary cause of sepsis in neonates, although CoNS are among a short list of “translocating” bacteria when present in abundance. Methods: 468 blood samples, 119 stool sample...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/9/8/2517 |
| _version_ | 1797560319458934784 |
|---|---|
| author | Edyta Golińska Magdalena Strus Anna Tomusiak-Plebanek Grażyna Więcek Łucja Kozień Ryszard Lauterbach Dorota Pawlik Beata Rzepecka-Węglarz Jolanta Kędzierska Małgorzata Dorycka Piotr B. Heczko |
| author_facet | Edyta Golińska Magdalena Strus Anna Tomusiak-Plebanek Grażyna Więcek Łucja Kozień Ryszard Lauterbach Dorota Pawlik Beata Rzepecka-Węglarz Jolanta Kędzierska Małgorzata Dorycka Piotr B. Heczko |
| author_sort | Edyta Golińska |
| collection | DOAJ |
| description | Background: There are only a few reports in the literature about translocation of coagulase-negative staphylococci (CoNS) as a primary cause of sepsis in neonates, although CoNS are among a short list of “translocating” bacteria when present in abundance. Methods: 468 blood samples, 119 stool samples, and 8 catheter tips, from 311 neonates, were tested for presence of microorganisms. CoNS strains isolated from the blood and stool or from blood and catheter tip of the same newborn at approximately the same time were paired and typed with PFGE (Pulse-Field Gel Electrophoresis) method. The strains were then tested for the presence of adherence genes and biofilm formation. Results: The strains with identical PFGE profiles in comparison to those with non-identical profiles differed in terms of the pattern of the virulence genes and showed a lack of the genes related to adherence, but more often presence of IS256, which is related to virulence. They also were phenotypically unable to adhere to intestinal Caco2 cells. Conclusions: A considerable proportion of CoNS strains isolated from bloodstream of VLBW/LWB neonates was identical to the strains isolated from faeces of the same neonates at the same time. These observations may offer indirect evidence indicating that at least some CoNS can translocate from the gastrointestinal tract of the premature neonates into the bloodstream and thus cause generalized infection. |
| first_indexed | 2024-03-10T17:57:54Z |
| format | Article |
| id | doaj.art-533fedcac06a412d88463c06fafdb959 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T17:57:54Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-533fedcac06a412d88463c06fafdb9592023-11-20T09:04:52ZengMDPI AGJournal of Clinical Medicine2077-03832020-08-0198251710.3390/jcm9082517Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight NeonatesEdyta Golińska0Magdalena Strus1Anna Tomusiak-Plebanek2Grażyna Więcek3Łucja Kozień4Ryszard Lauterbach5Dorota Pawlik6Beata Rzepecka-Węglarz7Jolanta Kędzierska8Małgorzata Dorycka9Piotr B. Heczko10Jagiellonian University Medical College, 31-121 Cracow, PolandDepartment of Neonatology, Jagiellonian University, Medical College, 31-501 Cracow, PolandJagiellonian University Medical College, 31-121 Cracow, PolandJagiellonian University Medical College, 31-121 Cracow, PolandJagiellonian University Medical College, 31-121 Cracow, PolandDepartment of Neonatology, Jagiellonian University, Medical College, 31-501 Cracow, PolandDepartment of Neonatology, Jagiellonian University, Medical College, 31-501 Cracow, PolandDepartment of Neonatal Intensive Care, “UJASTEK” Medical Centre, 31-752 Cracow, PolandDepartment of Microbiology, University Hospital, 31-501 Cracow, PolandMicrobiological Laboratory, Diagnostics Inc. Krakow Branch, 31-864 Cracow, PolandJagiellonian University Medical College, 31-121 Cracow, PolandBackground: There are only a few reports in the literature about translocation of coagulase-negative staphylococci (CoNS) as a primary cause of sepsis in neonates, although CoNS are among a short list of “translocating” bacteria when present in abundance. Methods: 468 blood samples, 119 stool samples, and 8 catheter tips, from 311 neonates, were tested for presence of microorganisms. CoNS strains isolated from the blood and stool or from blood and catheter tip of the same newborn at approximately the same time were paired and typed with PFGE (Pulse-Field Gel Electrophoresis) method. The strains were then tested for the presence of adherence genes and biofilm formation. Results: The strains with identical PFGE profiles in comparison to those with non-identical profiles differed in terms of the pattern of the virulence genes and showed a lack of the genes related to adherence, but more often presence of IS256, which is related to virulence. They also were phenotypically unable to adhere to intestinal Caco2 cells. Conclusions: A considerable proportion of CoNS strains isolated from bloodstream of VLBW/LWB neonates was identical to the strains isolated from faeces of the same neonates at the same time. These observations may offer indirect evidence indicating that at least some CoNS can translocate from the gastrointestinal tract of the premature neonates into the bloodstream and thus cause generalized infection.https://www.mdpi.com/2077-0383/9/8/2517sepsisCoNSstaphylococcusnewbornneonatestranslocation |
| spellingShingle | Edyta Golińska Magdalena Strus Anna Tomusiak-Plebanek Grażyna Więcek Łucja Kozień Ryszard Lauterbach Dorota Pawlik Beata Rzepecka-Węglarz Jolanta Kędzierska Małgorzata Dorycka Piotr B. Heczko Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates Journal of Clinical Medicine sepsis CoNS staphylococcus newborn neonates translocation |
| title | Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates |
| title_full | Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates |
| title_fullStr | Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates |
| title_full_unstemmed | Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates |
| title_short | Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates |
| title_sort | coagulase negative staphylococci contained in gut microbiota as a primary source of sepsis in low and very low birth weight neonates |
| topic | sepsis CoNS staphylococcus newborn neonates translocation |
| url | https://www.mdpi.com/2077-0383/9/8/2517 |
| work_keys_str_mv | AT edytagolinska coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT magdalenastrus coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT annatomusiakplebanek coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT grazynawiecek coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT łucjakozien coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT ryszardlauterbach coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT dorotapawlik coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT beatarzepeckaweglarz coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT jolantakedzierska coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT małgorzatadorycka coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates AT piotrbheczko coagulasenegativestaphylococcicontainedingutmicrobiotaasaprimarysourceofsepsisinlowandverylowbirthweightneonates |