Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil
Flax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids. Inactivating mu...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/1/95 |
| _version_ | 1797440056281006080 |
|---|---|
| author | Liubov V. Povkhova Elena N. Pushkova Tatiana A. Rozhmina Alexander A. Zhuchenko Roman I. Frykin Roman O. Novakovskiy Ekaterina M. Dvorianinova Aleksey A. Gryzunov Elena V. Borkhert Elizaveta A. Sigova Gleb N. Vladimirov Anastasiya V. Snezhkina Anna V. Kudryavtseva George S. Krasnov Alexey A. Dmitriev Nataliya V. Melnikova |
| author_facet | Liubov V. Povkhova Elena N. Pushkova Tatiana A. Rozhmina Alexander A. Zhuchenko Roman I. Frykin Roman O. Novakovskiy Ekaterina M. Dvorianinova Aleksey A. Gryzunov Elena V. Borkhert Elizaveta A. Sigova Gleb N. Vladimirov Anastasiya V. Snezhkina Anna V. Kudryavtseva George S. Krasnov Alexey A. Dmitriev Nataliya V. Melnikova |
| author_sort | Liubov V. Povkhova |
| collection | DOAJ |
| description | Flax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids. Inactivating mutations in the <i>FAD3A</i> and <i>FAD3B</i> genes lead to a decrease in the LIN content and an increase in the LIO content. For the identification of the three most common low-LIN mutations in flax varieties (G-to-A in exon 1 of <i>FAD3A</i> substituting tryptophan with a stop codon, C-to-T in exon 5 of <i>FAD3A</i> leading to arginine to a stop codon substitution, and C-to-T in exon 2 of <i>FAD3B</i> resulting in histidine to tyrosine substitution), three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers. They were tested on more than a thousand flax samples of various types and showed promising results. The proposed approaches can be used in marker-assisted selection to choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous alleles of the <i>FAD3A</i> and <i>FAD3B</i> genes at the early stages of breeding for the effective development of varieties with a particular LIN and LIO content, as well as in basic studies of the molecular mechanisms of fatty acid synthesis in flax seeds to select genotypes adequate to the tasks. |
| first_indexed | 2024-03-09T12:01:35Z |
| format | Article |
| id | doaj.art-534134c6c8ad403cbaa1bc98436c8938 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:01:35Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-534134c6c8ad403cbaa1bc98436c89382023-11-30T23:02:22ZengMDPI AGPlants2223-77472022-12-011219510.3390/plants12010095Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax OilLiubov V. Povkhova0Elena N. Pushkova1Tatiana A. Rozhmina2Alexander A. Zhuchenko3Roman I. Frykin4Roman O. Novakovskiy5Ekaterina M. Dvorianinova6Aleksey A. Gryzunov7Elena V. Borkhert8Elizaveta A. Sigova9Gleb N. Vladimirov10Anastasiya V. Snezhkina11Anna V. Kudryavtseva12George S. Krasnov13Alexey A. Dmitriev14Nataliya V. Melnikova15Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaFederal Research Center for Bast Fiber Crops, 172002 Torzhok, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaAll-Russian Scientific Research Institute of Refrigeration Industry—Branch of V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, 127422 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaSkolkovo Institute of Science and Technology, 121205 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaFlax is grown worldwide for seed and fiber production. Linseed varieties differ in their oil composition and are used in pharmaceutical, food, feed, and industrial production. The field of application primarily depends on the content of linolenic (LIN) and linoleic (LIO) fatty acids. Inactivating mutations in the <i>FAD3A</i> and <i>FAD3B</i> genes lead to a decrease in the LIN content and an increase in the LIO content. For the identification of the three most common low-LIN mutations in flax varieties (G-to-A in exon 1 of <i>FAD3A</i> substituting tryptophan with a stop codon, C-to-T in exon 5 of <i>FAD3A</i> leading to arginine to a stop codon substitution, and C-to-T in exon 2 of <i>FAD3B</i> resulting in histidine to tyrosine substitution), three approaches were proposed: (1) targeted deep sequencing, (2) high resolution melting (HRM) analysis, (3) cleaved amplified polymorphic sequences (CAPS) markers. They were tested on more than a thousand flax samples of various types and showed promising results. The proposed approaches can be used in marker-assisted selection to choose parent pairs for crosses, separate heterogeneous varieties into biotypes, and select genotypes with desired homozygous alleles of the <i>FAD3A</i> and <i>FAD3B</i> genes at the early stages of breeding for the effective development of varieties with a particular LIN and LIO content, as well as in basic studies of the molecular mechanisms of fatty acid synthesis in flax seeds to select genotypes adequate to the tasks.https://www.mdpi.com/2223-7747/12/1/95flaxlinseedfatty acid compositionlinolenic acid<i>FAD3</i> genesDNA markers |
| spellingShingle | Liubov V. Povkhova Elena N. Pushkova Tatiana A. Rozhmina Alexander A. Zhuchenko Roman I. Frykin Roman O. Novakovskiy Ekaterina M. Dvorianinova Aleksey A. Gryzunov Elena V. Borkhert Elizaveta A. Sigova Gleb N. Vladimirov Anastasiya V. Snezhkina Anna V. Kudryavtseva George S. Krasnov Alexey A. Dmitriev Nataliya V. Melnikova Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil Plants flax linseed fatty acid composition linolenic acid <i>FAD3</i> genes DNA markers |
| title | Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil |
| title_full | Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil |
| title_fullStr | Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil |
| title_full_unstemmed | Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil |
| title_short | Development and Complex Application of Methods for the Identification of Mutations in the <i>FAD3A</i> and <i>FAD3B</i> Genes Resulting in the Reduced Content of Linolenic Acid in Flax Oil |
| title_sort | development and complex application of methods for the identification of mutations in the i fad3a i and i fad3b i genes resulting in the reduced content of linolenic acid in flax oil |
| topic | flax linseed fatty acid composition linolenic acid <i>FAD3</i> genes DNA markers |
| url | https://www.mdpi.com/2223-7747/12/1/95 |
| work_keys_str_mv | AT liubovvpovkhova developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT elenanpushkova developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT tatianaarozhmina developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT alexanderazhuchenko developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT romanifrykin developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT romanonovakovskiy developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT ekaterinamdvorianinova developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT alekseyagryzunov developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT elenavborkhert developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT elizavetaasigova developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT glebnvladimirov developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT anastasiyavsnezhkina developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT annavkudryavtseva developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT georgeskrasnov developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT alexeyadmitriev developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil AT nataliyavmelnikova developmentandcomplexapplicationofmethodsfortheidentificationofmutationsintheifad3aiandifad3bigenesresultinginthereducedcontentoflinolenicacidinflaxoil |