Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas
Abstract Purpose To test the theory that invaginated ovarian surface epithelium and endometrial implants on the ovary form ovarian endometriomas. Methods Adhesion sites of ovarian endometrioma on the peritoneum and consecutive ovarian endometrioma cyst wall, called non‐adhesion sites, were histologi...
| Huvudupphovsmän: | , , , , , , , , , , , , , , , |
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| Materialtyp: | Artikel |
| Språk: | English |
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Wiley
2023-01-01
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| Serie: | Reproductive Medicine and Biology |
| Ämnen: | |
| Länkar: | https://doi.org/10.1002/rmb2.12548 |
| _version_ | 1827398882032615424 |
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| author | Ryo Maekawa Yoshiaki Ota Ikuko Ota Yumiko Mihara Hitomi Takasaki Shun Sato Isao Tamura Yuichiro Shirafuta Masahiro Shinagawa Taishi Fujimura Amon Shiroshita Toshihide Yoneda Mai Kawamoto‐Jozaki Fuka Matsui Toshiaki Taketani Norihiro Sugino |
| author_facet | Ryo Maekawa Yoshiaki Ota Ikuko Ota Yumiko Mihara Hitomi Takasaki Shun Sato Isao Tamura Yuichiro Shirafuta Masahiro Shinagawa Taishi Fujimura Amon Shiroshita Toshihide Yoneda Mai Kawamoto‐Jozaki Fuka Matsui Toshiaki Taketani Norihiro Sugino |
| author_sort | Ryo Maekawa |
| collection | DOAJ |
| description | Abstract Purpose To test the theory that invaginated ovarian surface epithelium and endometrial implants on the ovary form ovarian endometriomas. Methods Adhesion sites of ovarian endometrioma on the peritoneum and consecutive ovarian endometrioma cyst wall, called non‐adhesion sites, were histologically examined. DNA methylomes of the adhesion sites, non‐adhesion sites, and blueberry spots were compared with those of ovary, endometrium, and peritoneum. Results The non‐adhesion sites showed an ovarian surface epithelium‐like structure near the adhesion site, which continued to a columnar epithelium‐like structure. Calretinin staining was strong in the ovarian surface epithelium‐like structure but weak in the columnar epithelium‐like structure. Estrogen receptors were absent in the ovarian surface epithelium‐like structure, but present in the columnar epithelium‐like structure. The adhesion sites had endometrial gland‐like structures that expressed estrogen receptors. Analyses of DNA methylomes classified the non‐adhesion sites and ovaries into the same group, suggesting that ovarian endometriomas originate from the ovarian surface epithelium. The adhesion sites, blueberry spots and peritoneum were classified in the same group, suggesting that the adhesion sites and blueberry spots originate from the peritoneum. Conclusions The present results support the invagination theory. Ovarian endometriomas consist of invaginated ovarian surface epithelium with celomic metaplasia and endometrium implants on the peritoneum. |
| first_indexed | 2024-03-08T19:35:15Z |
| format | Article |
| id | doaj.art-53427fb8db954d98b9c99ce9c227f13b |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-03-08T19:35:15Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-53427fb8db954d98b9c99ce9c227f13b2023-12-26T04:30:44ZengWileyReproductive Medicine and Biology1445-57811447-05782023-01-01221n/an/a10.1002/rmb2.12548Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomasRyo Maekawa0Yoshiaki Ota1Ikuko Ota2Yumiko Mihara3Hitomi Takasaki4Shun Sato5Isao Tamura6Yuichiro Shirafuta7Masahiro Shinagawa8Taishi Fujimura9Amon Shiroshita10Toshihide Yoneda11Mai Kawamoto‐Jozaki12Fuka Matsui13Toshiaki Taketani14Norihiro Sugino15Department of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Kawasaki Medical School Kurashiki JapanIKuko Ota Women's Medical Center Kurashiki JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanDepartment of Obstetrics and Gynecology Yamaguchi University Graduate School of Medicine Ube JapanAbstract Purpose To test the theory that invaginated ovarian surface epithelium and endometrial implants on the ovary form ovarian endometriomas. Methods Adhesion sites of ovarian endometrioma on the peritoneum and consecutive ovarian endometrioma cyst wall, called non‐adhesion sites, were histologically examined. DNA methylomes of the adhesion sites, non‐adhesion sites, and blueberry spots were compared with those of ovary, endometrium, and peritoneum. Results The non‐adhesion sites showed an ovarian surface epithelium‐like structure near the adhesion site, which continued to a columnar epithelium‐like structure. Calretinin staining was strong in the ovarian surface epithelium‐like structure but weak in the columnar epithelium‐like structure. Estrogen receptors were absent in the ovarian surface epithelium‐like structure, but present in the columnar epithelium‐like structure. The adhesion sites had endometrial gland‐like structures that expressed estrogen receptors. Analyses of DNA methylomes classified the non‐adhesion sites and ovaries into the same group, suggesting that ovarian endometriomas originate from the ovarian surface epithelium. The adhesion sites, blueberry spots and peritoneum were classified in the same group, suggesting that the adhesion sites and blueberry spots originate from the peritoneum. Conclusions The present results support the invagination theory. Ovarian endometriomas consist of invaginated ovarian surface epithelium with celomic metaplasia and endometrium implants on the peritoneum.https://doi.org/10.1002/rmb2.12548DNA methylationhistological examinationmetaplasiaovarian endometriomaperitoneal endometriosis |
| spellingShingle | Ryo Maekawa Yoshiaki Ota Ikuko Ota Yumiko Mihara Hitomi Takasaki Shun Sato Isao Tamura Yuichiro Shirafuta Masahiro Shinagawa Taishi Fujimura Amon Shiroshita Toshihide Yoneda Mai Kawamoto‐Jozaki Fuka Matsui Toshiaki Taketani Norihiro Sugino Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas Reproductive Medicine and Biology DNA methylation histological examination metaplasia ovarian endometrioma peritoneal endometriosis |
| title | Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| title_full | Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| title_fullStr | Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| title_full_unstemmed | Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| title_short | Combined histological and DNA methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| title_sort | combined histological and dna methylome profiling approaches may provide insights into the pathophysiology of ovarian endometriomas |
| topic | DNA methylation histological examination metaplasia ovarian endometrioma peritoneal endometriosis |
| url | https://doi.org/10.1002/rmb2.12548 |
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