NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe
The changing epidemiology of carbapenem-resistant <i>Klebsiella pneumoniae</i> in Southern European countries is challenging for infection control, and it is critical to identify and track new genetic entities (genes, carbapenemases, clones) quickly and with high precision. We aimed to c...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/92 |
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| author | Ângela Novais Rita Veiga Ferraz Mariana Viana Paula Martins da Costa Luísa Peixe |
| author_facet | Ângela Novais Rita Veiga Ferraz Mariana Viana Paula Martins da Costa Luísa Peixe |
| author_sort | Ângela Novais |
| collection | DOAJ |
| description | The changing epidemiology of carbapenem-resistant <i>Klebsiella pneumoniae</i> in Southern European countries is challenging for infection control, and it is critical to identify and track new genetic entities (genes, carbapenemases, clones) quickly and with high precision. We aimed to characterize the strain responsible for the first recognized outbreak by an NDM-1-producing <i>K. pneumoniae</i> in Portugal, and to elucidate its diffusion in an international context. NDM-1-producing multidrug-resistant <i>K. pneumoniae</i> isolates from hospitalized patients (2018–2019) were characterized using FTIR spectroscopy, molecular typing, whole-genome sequencing, and comparative genomics with available <i>K. pneumoniae</i> ST11 KL105 genomes. FT-IR spectroscopy allowed the rapid (ca. 4 h after incubation) identification of the outbreak strains as ST11 KL105, supporting outbreak control. Epidemiological information supports a community source but without linkage to endemic regions of NDM-1 producers. Whole-genome comparison with previous DHA-1-producing ST11 KL105 strains revealed the presence of different plasmid types and antibiotic resistance traits, suggesting the entry of a new strain. In fact, this ST11 KL105 clade has successfully disseminated in Europe with variable beta-lactamases, but essentially as ESBL or DHA-1 producers. We expand the distribution map of NDM-1-producing <i>K. pneumoniae</i> in Europe, at the expense of a successfully established ST11 KL105 <i>K. pneumoniae</i> clade circulating with variable plasmid backgrounds and beta-lactamases. Our work further supports the use of FT-IR as an asset to support quick infection control. |
| first_indexed | 2024-03-10T03:01:40Z |
| format | Article |
| id | doaj.art-5347364174ec40b8b36bc2b9a830e62c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:01:40Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5347364174ec40b8b36bc2b9a830e62c2023-11-23T12:45:04ZengMDPI AGAntibiotics2079-63822022-01-011119210.3390/antibiotics11010092NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in EuropeÂngela Novais0Rita Veiga Ferraz1Mariana Viana2Paula Martins da Costa3Luísa Peixe4UCIBIO, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, PortugalCentro Hospitalar do Tâmega e Sousa, 4564-007 Penafiel, PortugalCentro Hospitalar do Tâmega e Sousa, 4564-007 Penafiel, PortugalCentro Hospitalar do Tâmega e Sousa, 4564-007 Penafiel, PortugalUCIBIO, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, PortugalThe changing epidemiology of carbapenem-resistant <i>Klebsiella pneumoniae</i> in Southern European countries is challenging for infection control, and it is critical to identify and track new genetic entities (genes, carbapenemases, clones) quickly and with high precision. We aimed to characterize the strain responsible for the first recognized outbreak by an NDM-1-producing <i>K. pneumoniae</i> in Portugal, and to elucidate its diffusion in an international context. NDM-1-producing multidrug-resistant <i>K. pneumoniae</i> isolates from hospitalized patients (2018–2019) were characterized using FTIR spectroscopy, molecular typing, whole-genome sequencing, and comparative genomics with available <i>K. pneumoniae</i> ST11 KL105 genomes. FT-IR spectroscopy allowed the rapid (ca. 4 h after incubation) identification of the outbreak strains as ST11 KL105, supporting outbreak control. Epidemiological information supports a community source but without linkage to endemic regions of NDM-1 producers. Whole-genome comparison with previous DHA-1-producing ST11 KL105 strains revealed the presence of different plasmid types and antibiotic resistance traits, suggesting the entry of a new strain. In fact, this ST11 KL105 clade has successfully disseminated in Europe with variable beta-lactamases, but essentially as ESBL or DHA-1 producers. We expand the distribution map of NDM-1-producing <i>K. pneumoniae</i> in Europe, at the expense of a successfully established ST11 KL105 <i>K. pneumoniae</i> clade circulating with variable plasmid backgrounds and beta-lactamases. Our work further supports the use of FT-IR as an asset to support quick infection control.https://www.mdpi.com/2079-6382/11/1/92nosocomial infectionscarbapenemase-producing EnterobacteralesNDM-1whole-genome sequencingoutbreakFourier transform infrared spectroscopy |
| spellingShingle | Ângela Novais Rita Veiga Ferraz Mariana Viana Paula Martins da Costa Luísa Peixe NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe Antibiotics nosocomial infections carbapenemase-producing Enterobacterales NDM-1 whole-genome sequencing outbreak Fourier transform infrared spectroscopy |
| title | NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe |
| title_full | NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe |
| title_fullStr | NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe |
| title_full_unstemmed | NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe |
| title_short | NDM-1 Introduction in Portugal through a ST11 KL105 <i>Klebsiella pneumoniae</i> Widespread in Europe |
| title_sort | ndm 1 introduction in portugal through a st11 kl105 i klebsiella pneumoniae i widespread in europe |
| topic | nosocomial infections carbapenemase-producing Enterobacterales NDM-1 whole-genome sequencing outbreak Fourier transform infrared spectroscopy |
| url | https://www.mdpi.com/2079-6382/11/1/92 |
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