Structural and Energetic Characterization of the Ankyrin Repeat Protein Family.
Ankyrin repeat containing proteins are one of the most abundant solenoid folds. Usually implicated in specific protein-protein interactions, these proteins are readily amenable for design, with promising biotechnological and biomedical applications. Studying repeat protein families presents technica...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-12-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC4687027?pdf=render |
| _version_ | 1828180063868157952 |
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| author | R Gonzalo Parra Rocío Espada Nina Verstraete Diego U Ferreiro |
| author_facet | R Gonzalo Parra Rocío Espada Nina Verstraete Diego U Ferreiro |
| author_sort | R Gonzalo Parra |
| collection | DOAJ |
| description | Ankyrin repeat containing proteins are one of the most abundant solenoid folds. Usually implicated in specific protein-protein interactions, these proteins are readily amenable for design, with promising biotechnological and biomedical applications. Studying repeat protein families presents technical challenges due to the high sequence divergence among the repeating units. We developed and applied a systematic method to consistently identify and annotate the structural repetitions over the members of the complete Ankyrin Repeat Protein Family, with increased sensitivity over previous studies. We statistically characterized the number of repeats, the folding of the repeat-arrays, their structural variations, insertions and deletions. An energetic analysis of the local frustration patterns reveal the basic features underlying fold stability and its relation to the functional binding regions. We found a strong linear correlation between the conservation of the energetic features in the repeat arrays and their sequence variations, and discuss new insights into the organization and function of these ubiquitous proteins. |
| first_indexed | 2024-04-12T05:38:41Z |
| format | Article |
| id | doaj.art-5347960a9587449ba8ac623d6f36a674 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-04-12T05:38:41Z |
| publishDate | 2015-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-5347960a9587449ba8ac623d6f36a6742022-12-22T03:45:43ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582015-12-011112e100465910.1371/journal.pcbi.1004659Structural and Energetic Characterization of the Ankyrin Repeat Protein Family.R Gonzalo ParraRocío EspadaNina VerstraeteDiego U FerreiroAnkyrin repeat containing proteins are one of the most abundant solenoid folds. Usually implicated in specific protein-protein interactions, these proteins are readily amenable for design, with promising biotechnological and biomedical applications. Studying repeat protein families presents technical challenges due to the high sequence divergence among the repeating units. We developed and applied a systematic method to consistently identify and annotate the structural repetitions over the members of the complete Ankyrin Repeat Protein Family, with increased sensitivity over previous studies. We statistically characterized the number of repeats, the folding of the repeat-arrays, their structural variations, insertions and deletions. An energetic analysis of the local frustration patterns reveal the basic features underlying fold stability and its relation to the functional binding regions. We found a strong linear correlation between the conservation of the energetic features in the repeat arrays and their sequence variations, and discuss new insights into the organization and function of these ubiquitous proteins.http://europepmc.org/articles/PMC4687027?pdf=render |
| spellingShingle | R Gonzalo Parra Rocío Espada Nina Verstraete Diego U Ferreiro Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. PLoS Computational Biology |
| title | Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. |
| title_full | Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. |
| title_fullStr | Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. |
| title_full_unstemmed | Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. |
| title_short | Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. |
| title_sort | structural and energetic characterization of the ankyrin repeat protein family |
| url | http://europepmc.org/articles/PMC4687027?pdf=render |
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