Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae)
The Asian citrus psyllid<i>, Diaphorina citri</i> Kuwayama is devastating the citrus industry worldwide. It transmits ‘<i>Candidatus</i> Liberibacter asiaticus’, the pathogen of Huanglongbing in citrus. RNA interference is an excellent tool for functional genomics and for scr...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/10/931 |
| _version_ | 1797514239559073792 |
|---|---|
| author | Yulica Santos-Ortega Nabil Killiny |
| author_facet | Yulica Santos-Ortega Nabil Killiny |
| author_sort | Yulica Santos-Ortega |
| collection | DOAJ |
| description | The Asian citrus psyllid<i>, Diaphorina citri</i> Kuwayama is devastating the citrus industry worldwide. It transmits ‘<i>Candidatus</i> Liberibacter asiaticus’, the pathogen of Huanglongbing in citrus. RNA interference is an excellent tool for functional genomics and for screening target genes for pest control. Herein, we silenced the aquaporin (AQP) gene (<i>DcAQP</i>) homologue in <i>D. citri</i> to study its functionality and whether it could be a good target for a control strategy. AQP is an integral membrane channel protein that aids in the rapid flux of water and other small solutes that move across the lipid membrane. In Hemiptera, it is well established that AQP plays important roles in adjusting to physiological challenges including (1) regulating osmotic stress between the gut lumen and hemolymph after imbibing large quantities of a low nitrogen, sugar-rich liquid diet; (2) avoiding or preventing dehydration and desiccation; and (3) surviving at elevated temperatures. The dsRNA-<i>DcAQP</i> was applied twice to nymphs of the 4th and 5th instars through a soaking technique. Silencing AQP caused a significant increase in nymph mortality. Emerged adults showed malformations and a shorter lifespan. Silencing <i>DcAQP</i> provoked alterations in some metabolites and increased the uric acid content in emerged adults. <i>DcAQP</i> could be a useful target to control <i>D. citri</i>. |
| first_indexed | 2024-03-10T06:28:48Z |
| format | Article |
| id | doaj.art-53481977990b453691052f4ca2fa3067 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T06:28:48Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-53481977990b453691052f4ca2fa30672023-11-22T18:39:51ZengMDPI AGInsects2075-44502021-10-01121093110.3390/insects12100931Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae)Yulica Santos-Ortega0Nabil Killiny1Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USADepartment of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USAThe Asian citrus psyllid<i>, Diaphorina citri</i> Kuwayama is devastating the citrus industry worldwide. It transmits ‘<i>Candidatus</i> Liberibacter asiaticus’, the pathogen of Huanglongbing in citrus. RNA interference is an excellent tool for functional genomics and for screening target genes for pest control. Herein, we silenced the aquaporin (AQP) gene (<i>DcAQP</i>) homologue in <i>D. citri</i> to study its functionality and whether it could be a good target for a control strategy. AQP is an integral membrane channel protein that aids in the rapid flux of water and other small solutes that move across the lipid membrane. In Hemiptera, it is well established that AQP plays important roles in adjusting to physiological challenges including (1) regulating osmotic stress between the gut lumen and hemolymph after imbibing large quantities of a low nitrogen, sugar-rich liquid diet; (2) avoiding or preventing dehydration and desiccation; and (3) surviving at elevated temperatures. The dsRNA-<i>DcAQP</i> was applied twice to nymphs of the 4th and 5th instars through a soaking technique. Silencing AQP caused a significant increase in nymph mortality. Emerged adults showed malformations and a shorter lifespan. Silencing <i>DcAQP</i> provoked alterations in some metabolites and increased the uric acid content in emerged adults. <i>DcAQP</i> could be a useful target to control <i>D. citri</i>.https://www.mdpi.com/2075-4450/12/10/931Asian citrus psyllid<i>Diaphorina citri</i>RNA interferencegene silencingaquaporinuric acid |
| spellingShingle | Yulica Santos-Ortega Nabil Killiny Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) Insects Asian citrus psyllid <i>Diaphorina citri</i> RNA interference gene silencing aquaporin uric acid |
| title | Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) |
| title_full | Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) |
| title_fullStr | Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) |
| title_full_unstemmed | Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) |
| title_short | Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, <i>Diaphorina citri</i> (Hemiptera: Liviidae) |
| title_sort | silencing of aquaporin homologue accumulates uric acid and decreases the lifespan of the asian citrus psyllid i diaphorina citri i hemiptera liviidae |
| topic | Asian citrus psyllid <i>Diaphorina citri</i> RNA interference gene silencing aquaporin uric acid |
| url | https://www.mdpi.com/2075-4450/12/10/931 |
| work_keys_str_mv | AT yulicasantosortega silencingofaquaporinhomologueaccumulatesuricacidanddecreasesthelifespanoftheasiancitruspsyllididiaphorinacitriihemipteraliviidae AT nabilkilliny silencingofaquaporinhomologueaccumulatesuricacidanddecreasesthelifespanoftheasiancitruspsyllididiaphorinacitriihemipteraliviidae |