Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases
Cardiovascular diseases (CVD) affect a large number of the population across the globe and are the leading cause of death worldwide. Nanotechnology-based drug delivery has currently offered novel therapeutic options to treat these diseases, yet combination of both diagnostic and therapeutic abilitie...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00353/full |
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| author | Aneetta E. Kuriakose Nikhil Pandey Dingying Shan Subhash Banerjee Subhash Banerjee Jian Yang Kytai T. Nguyen |
| author_facet | Aneetta E. Kuriakose Nikhil Pandey Dingying Shan Subhash Banerjee Subhash Banerjee Jian Yang Kytai T. Nguyen |
| author_sort | Aneetta E. Kuriakose |
| collection | DOAJ |
| description | Cardiovascular diseases (CVD) affect a large number of the population across the globe and are the leading cause of death worldwide. Nanotechnology-based drug delivery has currently offered novel therapeutic options to treat these diseases, yet combination of both diagnostic and therapeutic abilities is further needed to understand factors and/or mechanisms that affect the treatment in order to design better therapies to challenge CVD. Biodegradable photoluminescent polylactones (BPLPLs) enable to bridge this gap as these materials exhibit a stable, long-term intrinsic fluorescence as well as offers excellent cytocompatibility and biodegradability properties. Herein, we formulated three different BPLPL based nanoparticles (NPs), including BPLP-co-poly (L-lactic acid) (BPLPL-PLLA), BPLP-co-poly (lactic-co-glycolic acid) copolymers with lactic acid and glycolic acid ratios of 75:25 (BPLPL-PLGA75:25) and 50:50 (BPLPL-PLGA50:50), and extensively evaluated their suitability as theranostic nanocarriers for CVD applications. All BPLPL based NPs were <160 nm in size and had photoluminescence characteristics and tunable release kinetics of encapsulated protein model depending on polylactones copolymerized with BPLP materials. Compared to BPLPL-PLLA NPs, BPLPL-PLGA NPs demonstrated excellent stability in various formulations including deionized water, serum, saline, and simulated body fluid over 2 days. In vitro cell studies with human umbilical vein derived endothelial cells showed dose-dependent accumulation of BPLPL-based NPs, and BPLPL-PLGA NPs presented superior compatibility with endothelial cells in terms of viability with minimal effects on cellular functions such as nitric oxide production. Furthermore, all BPLPL NPs displayed hemocompatibility with no effect on whole blood kinetic profiles, were non-hemolytic, and consisted of comparable platelet responses such as platelet adhesion and activation to those of PLGA, an FDA approved material. Overall, our results demonstrated that BPLPL-PLGA based NPs have better physical and biological properties than BPLPL-PLLA; hence they have potential to be utilized as functional nanocarriers for therapy and diagnosis of CVD. |
| first_indexed | 2024-12-19T03:15:40Z |
| format | Article |
| id | doaj.art-53483e5e07664c0286589bfcc5becc72 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-19T03:15:40Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-53483e5e07664c0286589bfcc5becc722022-12-21T20:37:54ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-11-01710.3389/fbioe.2019.00353499081Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular DiseasesAneetta E. Kuriakose0Nikhil Pandey1Dingying Shan2Subhash Banerjee3Subhash Banerjee4Jian Yang5Kytai T. Nguyen6Bioengineering Department, The University of Texas at Arlington, Arlington, TX, United StatesBioengineering Department, The University of Texas at Arlington, Arlington, TX, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesDivision of Cardiology, VA North Texas Medical Center, Dallas, TX, United StatesDepartment of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA, United StatesBioengineering Department, The University of Texas at Arlington, Arlington, TX, United StatesCardiovascular diseases (CVD) affect a large number of the population across the globe and are the leading cause of death worldwide. Nanotechnology-based drug delivery has currently offered novel therapeutic options to treat these diseases, yet combination of both diagnostic and therapeutic abilities is further needed to understand factors and/or mechanisms that affect the treatment in order to design better therapies to challenge CVD. Biodegradable photoluminescent polylactones (BPLPLs) enable to bridge this gap as these materials exhibit a stable, long-term intrinsic fluorescence as well as offers excellent cytocompatibility and biodegradability properties. Herein, we formulated three different BPLPL based nanoparticles (NPs), including BPLP-co-poly (L-lactic acid) (BPLPL-PLLA), BPLP-co-poly (lactic-co-glycolic acid) copolymers with lactic acid and glycolic acid ratios of 75:25 (BPLPL-PLGA75:25) and 50:50 (BPLPL-PLGA50:50), and extensively evaluated their suitability as theranostic nanocarriers for CVD applications. All BPLPL based NPs were <160 nm in size and had photoluminescence characteristics and tunable release kinetics of encapsulated protein model depending on polylactones copolymerized with BPLP materials. Compared to BPLPL-PLLA NPs, BPLPL-PLGA NPs demonstrated excellent stability in various formulations including deionized water, serum, saline, and simulated body fluid over 2 days. In vitro cell studies with human umbilical vein derived endothelial cells showed dose-dependent accumulation of BPLPL-based NPs, and BPLPL-PLGA NPs presented superior compatibility with endothelial cells in terms of viability with minimal effects on cellular functions such as nitric oxide production. Furthermore, all BPLPL NPs displayed hemocompatibility with no effect on whole blood kinetic profiles, were non-hemolytic, and consisted of comparable platelet responses such as platelet adhesion and activation to those of PLGA, an FDA approved material. Overall, our results demonstrated that BPLPL-PLGA based NPs have better physical and biological properties than BPLPL-PLLA; hence they have potential to be utilized as functional nanocarriers for therapy and diagnosis of CVD.https://www.frontiersin.org/article/10.3389/fbioe.2019.00353/fullBPLPbioimagingtoxicityvascular drug carriersendothelial cellscardiovascular disease |
| spellingShingle | Aneetta E. Kuriakose Nikhil Pandey Dingying Shan Subhash Banerjee Subhash Banerjee Jian Yang Kytai T. Nguyen Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases Frontiers in Bioengineering and Biotechnology BPLP bioimaging toxicity vascular drug carriers endothelial cells cardiovascular disease |
| title | Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases |
| title_full | Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases |
| title_fullStr | Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases |
| title_full_unstemmed | Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases |
| title_short | Characterization of Photoluminescent Polylactone-Based Nanoparticles for Their Applications in Cardiovascular Diseases |
| title_sort | characterization of photoluminescent polylactone based nanoparticles for their applications in cardiovascular diseases |
| topic | BPLP bioimaging toxicity vascular drug carriers endothelial cells cardiovascular disease |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00353/full |
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