A facile and specific assay for quantifying microRNA by an optimized RT-qPCR approach.

A facile and specific assay for quantifying microRNA by an optimized RT-qPCR approach.

BACKGROUND: The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used meth...

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Bibliographic Details
Main Authors: Qian Mei, Xiang Li, Yuanguang Meng, Zhiqiang Wu, Mingzhou Guo, Yali Zhao, Xiaobing Fu, Weidong Han
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3465266?pdf=render

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