Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease
Summary: Paneth cells (PC) play a key role in the innate immune response of intestine epithelium, and PC defects contribute to the pathogenesis of Crohn’s disease (CD). In this study, we utilized active CD tissues and advanced oxidation protein products (AOPP)-challenged C57BL/6 mouse model to inves...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223013895 |
| _version_ | 1797774807709777920 |
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| author | Jie Shi Weidong Wang Shibo Sun Xiaoping Xu Jieying Fei Qian Zhou Caolitao Qin Shiyu Ou Fengfei Wu Fang ting Wu Tianyan Xu Lan Bai Fang Xie |
| author_facet | Jie Shi Weidong Wang Shibo Sun Xiaoping Xu Jieying Fei Qian Zhou Caolitao Qin Shiyu Ou Fengfei Wu Fang ting Wu Tianyan Xu Lan Bai Fang Xie |
| author_sort | Jie Shi |
| collection | DOAJ |
| description | Summary: Paneth cells (PC) play a key role in the innate immune response of intestine epithelium, and PC defects contribute to the pathogenesis of Crohn’s disease (CD). In this study, we utilized active CD tissues and advanced oxidation protein products (AOPP)-challenged C57BL/6 mouse model to investigate the effect of AOPP on PC defects in CD. We found that AOPP accumulated in active CD tissues and was negatively associated with lysozyme expression, while positively correlated with the presence of ER stress markers. Furthermore, AOPP treatment induced PC defects mainly through excessive ER stress in vivo, and AOPP also caused mitochondria-associated ER membranes formation and mitochondrial dysfunction. In addition, the effects of AOPP could be attenuated by the administration of ER stress inhibitor, TUDCA. These findings suggest a pathogenic role of AOPP contributing to PC defects and may provide the basis for developing new strategies to managing CD. |
| first_indexed | 2024-03-12T22:26:35Z |
| format | Article |
| id | doaj.art-53524e5020804f5c9a72d84907c5d731 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:26:35Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-53524e5020804f5c9a72d84907c5d7312023-07-22T04:52:31ZengElsevieriScience2589-00422023-08-01268107312Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's diseaseJie Shi0Weidong Wang1Shibo Sun2Xiaoping Xu3Jieying Fei4Qian Zhou5Caolitao Qin6Shiyu Ou7Fengfei Wu8Fang ting Wu9Tianyan Xu10Lan Bai11Fang Xie12Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Department of Radiation Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaDepartment of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaDepartment of Gastroenterology, Hunan Provincial People’s Hospital, Changsha, Hunan 410005, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Department of Radiation Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, ChinaGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Corresponding authorGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Corresponding authorSummary: Paneth cells (PC) play a key role in the innate immune response of intestine epithelium, and PC defects contribute to the pathogenesis of Crohn’s disease (CD). In this study, we utilized active CD tissues and advanced oxidation protein products (AOPP)-challenged C57BL/6 mouse model to investigate the effect of AOPP on PC defects in CD. We found that AOPP accumulated in active CD tissues and was negatively associated with lysozyme expression, while positively correlated with the presence of ER stress markers. Furthermore, AOPP treatment induced PC defects mainly through excessive ER stress in vivo, and AOPP also caused mitochondria-associated ER membranes formation and mitochondrial dysfunction. In addition, the effects of AOPP could be attenuated by the administration of ER stress inhibitor, TUDCA. These findings suggest a pathogenic role of AOPP contributing to PC defects and may provide the basis for developing new strategies to managing CD.http://www.sciencedirect.com/science/article/pii/S2589004223013895Cell biology |
| spellingShingle | Jie Shi Weidong Wang Shibo Sun Xiaoping Xu Jieying Fei Qian Zhou Caolitao Qin Shiyu Ou Fengfei Wu Fang ting Wu Tianyan Xu Lan Bai Fang Xie Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease iScience Cell biology |
| title | Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease |
| title_full | Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease |
| title_fullStr | Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease |
| title_full_unstemmed | Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease |
| title_short | Advanced oxidation protein products induce Paneth cells defects by endoplasmic reticulum stress in Crohn's disease |
| title_sort | advanced oxidation protein products induce paneth cells defects by endoplasmic reticulum stress in crohn s disease |
| topic | Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223013895 |
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