Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum

Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum

The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene. First, the enhanced green fluorescent protein gene (egfp) and chloramphenicol resistance gene (Cmr), along with the two loxP loci, were in...

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Bibliographic Details
Main Authors: Hengyi Sun, Hao Chen, Xiaonan Zang, Pan Hou, Bingbing Zhou, Yuantao Liu, Fei Wu, Xiaofei Cao, Xuecheng Zhang
Format: Article
Language:English
Published: MDPI AG 2015-06-01
Series:Molecules
Subjects:
antibiotic resistance marker gene
Aurantiochytrium limacinum
Cre/loxP site-specific recombination system
homologous recombination
Online Access:http://www.mdpi.com/1420-3049/20/6/10110

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