Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range
<i>Juniperus sabina</i> L. var. <i>balkanensis</i> R.P. Adams & Tashev is a recently described endemic variety from the Balkan Peninsula. Its strong sprouting ability and fast vegetative propagation, on one hand, and fragmented distribution, on the other, can lead to lowe...
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2022-12-01
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| author | Nemanja Rajčević Tanja Dodoš Smiljana Janković Pedja Janaćković Valtcho D. Zheljazkov Petar D. Marin |
| author_facet | Nemanja Rajčević Tanja Dodoš Smiljana Janković Pedja Janaćković Valtcho D. Zheljazkov Petar D. Marin |
| author_sort | Nemanja Rajčević |
| collection | DOAJ |
| description | <i>Juniperus sabina</i> L. var. <i>balkanensis</i> R.P. Adams & Tashev is a recently described endemic variety from the Balkan Peninsula. Its strong sprouting ability and fast vegetative propagation, on one hand, and fragmented distribution, on the other, can lead to lower genetic diversity in local populations and to the differentiation of populations. As there has been no detailed investigation of this variety, we studied Balkan natural populations using phytochemical and molecular markers. Leaf essential oils (EOs) were chosen based on their proven usability in the population studies of <i>Juniperus</i> taxa, while ISSRs (Inter Simple Sequence Repeats) have been used due to their high resolution. In addition, since this variety is best described using molecular markers, the chloroplast <i>trn</i>S-<i>trn</i>G region was amplified from individuals from different populations having different chemotypes. Based on the essential oil profile, three chemotypes could be identified with a difference in their distribution. The analysis of molecular variance showed moderate differentiation of populations and regions, attesting to the start of the separation of three regions in the Balkans: west, east and south. The bioclimatic and environmental parameters and sex of the individual did not influence the EO profile, although some of the compounds present in low-to-medium concentrations showed strong correlation with several bioclimatic parameters. |
| first_indexed | 2024-03-09T17:07:17Z |
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| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T17:07:17Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-535c7d2f699249da8a208fcab27168222023-11-24T14:22:55ZengMDPI AGDiversity1424-28182022-12-011412106210.3390/d14121062Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural RangeNemanja Rajčević0Tanja Dodoš1Smiljana Janković2Pedja Janaćković3Valtcho D. Zheljazkov4Petar D. Marin5Faculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, University of Belgrade, 11000 Belgrade, SerbiaCrop and Soil Science Department, Oregon State University, Corvallis, OR 97331, USAFaculty of Biology, University of Belgrade, 11000 Belgrade, Serbia<i>Juniperus sabina</i> L. var. <i>balkanensis</i> R.P. Adams & Tashev is a recently described endemic variety from the Balkan Peninsula. Its strong sprouting ability and fast vegetative propagation, on one hand, and fragmented distribution, on the other, can lead to lower genetic diversity in local populations and to the differentiation of populations. As there has been no detailed investigation of this variety, we studied Balkan natural populations using phytochemical and molecular markers. Leaf essential oils (EOs) were chosen based on their proven usability in the population studies of <i>Juniperus</i> taxa, while ISSRs (Inter Simple Sequence Repeats) have been used due to their high resolution. In addition, since this variety is best described using molecular markers, the chloroplast <i>trn</i>S-<i>trn</i>G region was amplified from individuals from different populations having different chemotypes. Based on the essential oil profile, three chemotypes could be identified with a difference in their distribution. The analysis of molecular variance showed moderate differentiation of populations and regions, attesting to the start of the separation of three regions in the Balkans: west, east and south. The bioclimatic and environmental parameters and sex of the individual did not influence the EO profile, although some of the compounds present in low-to-medium concentrations showed strong correlation with several bioclimatic parameters.https://www.mdpi.com/1424-2818/14/12/1062savin juniperessential oilsISSRcpDNAenvironmental factors |
| spellingShingle | Nemanja Rajčević Tanja Dodoš Smiljana Janković Pedja Janaćković Valtcho D. Zheljazkov Petar D. Marin Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range Diversity savin juniper essential oils ISSR cpDNA environmental factors |
| title | Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range |
| title_full | Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range |
| title_fullStr | Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range |
| title_full_unstemmed | Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range |
| title_short | Molecular and Phytochemical Variability of Endemic <i>Juniperus sabina</i> var. <i>balkanensis</i> from Its Natural Range |
| title_sort | molecular and phytochemical variability of endemic i juniperus sabina i var i balkanensis i from its natural range |
| topic | savin juniper essential oils ISSR cpDNA environmental factors |
| url | https://www.mdpi.com/1424-2818/14/12/1062 |
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