Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy
Background Bile duct ligation (BDL) has been used for experimental research on hepatic encephalopathy (HE) caused by chronic liver disease. However, little research has been done on a BDL model in C57BL/6 mouse. Therefore, we evaluated the suitability of a BDL model in C57BL/6 mouse for the study of...
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Korean Society of Anesthesiologists
2020-01-01
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| Series: | Anesthesia and Pain Medicine |
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| Online Access: | http://www.anesth-pain-med.org/upload/pdf/APM-15-019.pdf |
| _version_ | 1797825454088912896 |
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| author | Inja Cho Bon-Nyeo Koo Eun Hee Kam Su Kyoung Lee Hanseul Oh So Yeon Kim |
| author_facet | Inja Cho Bon-Nyeo Koo Eun Hee Kam Su Kyoung Lee Hanseul Oh So Yeon Kim |
| author_sort | Inja Cho |
| collection | DOAJ |
| description | Background Bile duct ligation (BDL) has been used for experimental research on hepatic encephalopathy (HE) caused by chronic liver disease. However, little research has been done on a BDL model in C57BL/6 mouse. Therefore, we evaluated the suitability of a BDL model in C57BL/6 mouse for the study of HE and determined which behavioral tests are appropriate for the identification of HE in this model. Methods Twelve to fourteen-week-old male C57BL/6 mice were randomly assigned to either sham group or BDL group. Histological changes in liver were confirmed by hematoxylin/eosin and Masson’s trichrome staining. Liver function alterations were detected by alanine aminotransferase (ALT) and ammonia levels. To identify behavioral changes, open field, elevated plus maze, novel object recognition, and passive avoidance tests were performed. Results Inflammatory liver injury and fibrosis were observed 14 days after BDL. ALT and ammonia levels were significantly higher in BDL group than in sham group. There were no differences in general locomotor activity or anxiety between the groups. No difference was observed between these two groups in the novel object recognition test, but BDL group showed significant learning/memory impairment in the passive avoidance test compared to sham group. Conclusions Fourteen days of BDL in 12–14-week-old male C57BL/6 mice is a clinically relevant model for HE, as these mice have liver fibrosis with impaired liver function, hyperammonemia, and learning/memory impairment. Passive avoidance can be used as the major behavioral test in this model of HE. |
| first_indexed | 2024-03-13T10:54:15Z |
| format | Article |
| id | doaj.art-535d3f2bf2534e45a58222d9c29acbfa |
| institution | Directory Open Access Journal |
| issn | 1975-5171 2383-7977 |
| language | English |
| last_indexed | 2024-03-13T10:54:15Z |
| publishDate | 2020-01-01 |
| publisher | Korean Society of Anesthesiologists |
| record_format | Article |
| series | Anesthesia and Pain Medicine |
| spelling | doaj.art-535d3f2bf2534e45a58222d9c29acbfa2023-05-17T05:28:54ZengKorean Society of AnesthesiologistsAnesthesia and Pain Medicine1975-51712383-79772020-01-01151192710.17085/apm.2020.15.1.19975Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathyInja Cho0Bon-Nyeo Koo1Eun Hee Kam2Su Kyoung Lee3Hanseul Oh4So Yeon Kim5Department of Anesthesiology and Pain Medicine, Seoul, KoreaDepartment of Anesthesiology and Pain Medicine, Seoul, KoreaDepartment of Anesthesiology and Pain Medicine, Seoul, KoreaDepartment of Anesthesiology and Pain Medicine, Seoul, KoreaDepartment of Anesthesiology and Pain Medicine, Seoul, KoreaDepartment of Anesthesiology and Pain Medicine, Seoul, KoreaBackground Bile duct ligation (BDL) has been used for experimental research on hepatic encephalopathy (HE) caused by chronic liver disease. However, little research has been done on a BDL model in C57BL/6 mouse. Therefore, we evaluated the suitability of a BDL model in C57BL/6 mouse for the study of HE and determined which behavioral tests are appropriate for the identification of HE in this model. Methods Twelve to fourteen-week-old male C57BL/6 mice were randomly assigned to either sham group or BDL group. Histological changes in liver were confirmed by hematoxylin/eosin and Masson’s trichrome staining. Liver function alterations were detected by alanine aminotransferase (ALT) and ammonia levels. To identify behavioral changes, open field, elevated plus maze, novel object recognition, and passive avoidance tests were performed. Results Inflammatory liver injury and fibrosis were observed 14 days after BDL. ALT and ammonia levels were significantly higher in BDL group than in sham group. There were no differences in general locomotor activity or anxiety between the groups. No difference was observed between these two groups in the novel object recognition test, but BDL group showed significant learning/memory impairment in the passive avoidance test compared to sham group. Conclusions Fourteen days of BDL in 12–14-week-old male C57BL/6 mice is a clinically relevant model for HE, as these mice have liver fibrosis with impaired liver function, hyperammonemia, and learning/memory impairment. Passive avoidance can be used as the major behavioral test in this model of HE.http://www.anesth-pain-med.org/upload/pdf/APM-15-019.pdfbile duct ligationc57bl/6 mousehepatic encephalopathyliver dysfunctionpassive avoidance test |
| spellingShingle | Inja Cho Bon-Nyeo Koo Eun Hee Kam Su Kyoung Lee Hanseul Oh So Yeon Kim Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy Anesthesia and Pain Medicine bile duct ligation c57bl/6 mouse hepatic encephalopathy liver dysfunction passive avoidance test |
| title | Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy |
| title_full | Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy |
| title_fullStr | Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy |
| title_full_unstemmed | Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy |
| title_short | Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy |
| title_sort | bile duct ligation of c57bl 6 mice as a model of hepatic encephalopathy |
| topic | bile duct ligation c57bl/6 mouse hepatic encephalopathy liver dysfunction passive avoidance test |
| url | http://www.anesth-pain-med.org/upload/pdf/APM-15-019.pdf |
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