SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides
The viruses from the genus Begomovirus, family Geminiviridae are considered emergent pathogens, mainly because of the population explosion of their insect vectors. For this reason, more attention needs to be directed to the correct virus species identification inside the genus. The present paper had...
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Universidade Federal Rural do Semi-Árido
2012-01-01
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| Series: | Revista Caatinga |
| Online Access: | http://www.redalyc.org/articulo.oa?id=237123825003 |
| _version_ | 1819081931101306880 |
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| author | JOSÉ ALBERSIO DE ARAUJO LIMA ALINE KELLY QUEIROZ DO NASCIMENTO VANUZIA BATISTA OLIVEIRA AMORIM PAULA RADAELLI JOAQUIM TORRES FILHO |
| author_facet | JOSÉ ALBERSIO DE ARAUJO LIMA ALINE KELLY QUEIROZ DO NASCIMENTO VANUZIA BATISTA OLIVEIRA AMORIM PAULA RADAELLI JOAQUIM TORRES FILHO |
| author_sort | JOSÉ ALBERSIO DE ARAUJO LIMA |
| collection | DOAJ |
| description | The viruses from the genus Begomovirus, family Geminiviridae are considered emergent pathogens, mainly because of the population explosion of their insect vectors. For this reason, more attention needs to be directed to the correct virus species identification inside the genus. The present paper had the objectives of serologically detecting a begomovirus in Macroptilium lathyroides plants in the State of Ceará, and developing biological, serological and molecular studies with a virus isolate obtained from M. lathyroides. Indirect ELISA with antiserum for Macroptilium golden mosaic virus (MaGMV) demonstrated that the samples collected from M. lathyroides showing golden mosaic in the field were infected with a begomovirus. The virus isolate obtained was transmitted by grafting to eight species of the family Leguminosae, four species of Solonaceae, and one species in the family Amaranthaceae. The virus also was transmitted from M. lathyroides to M. lathyroides by the whitefly Bemisia tabaci biotype B. A DNA fragment of 1.2 kb was obtained by PCR with the primers PAL1v 1978 and PAR1c 496 for component A, and a DNA fragment of 0.5 kb was obtained with the primers PBL1v 2040 and PCR cl for component B, confirming the presence of a begomovirus infecting M. lathyroides. Molecular studies indicated that the begomovirus isolate showed 77% genomic similarity with Bean golden mosaic virus and 75% with Cowpea golden mosaic virus for their cp and rep genes, indicating the possibility that the isolate is a distinct virus species of the Begomovirus genus. |
| first_indexed | 2024-12-21T20:08:37Z |
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| id | doaj.art-535e4af06f9245faa038499244124f88 |
| institution | Directory Open Access Journal |
| issn | 0100-316X 1983-2125 |
| language | English |
| last_indexed | 2024-12-21T20:08:37Z |
| publishDate | 2012-01-01 |
| publisher | Universidade Federal Rural do Semi-Árido |
| record_format | Article |
| series | Revista Caatinga |
| spelling | doaj.art-535e4af06f9245faa038499244124f882022-12-21T18:51:46ZengUniversidade Federal Rural do Semi-ÁridoRevista Caatinga0100-316X1983-21252012-01-012521420SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroidesJOSÉ ALBERSIO DE ARAUJO LIMAALINE KELLY QUEIROZ DO NASCIMENTOVANUZIA BATISTA OLIVEIRA AMORIMPAULA RADAELLIJOAQUIM TORRES FILHOThe viruses from the genus Begomovirus, family Geminiviridae are considered emergent pathogens, mainly because of the population explosion of their insect vectors. For this reason, more attention needs to be directed to the correct virus species identification inside the genus. The present paper had the objectives of serologically detecting a begomovirus in Macroptilium lathyroides plants in the State of Ceará, and developing biological, serological and molecular studies with a virus isolate obtained from M. lathyroides. Indirect ELISA with antiserum for Macroptilium golden mosaic virus (MaGMV) demonstrated that the samples collected from M. lathyroides showing golden mosaic in the field were infected with a begomovirus. The virus isolate obtained was transmitted by grafting to eight species of the family Leguminosae, four species of Solonaceae, and one species in the family Amaranthaceae. The virus also was transmitted from M. lathyroides to M. lathyroides by the whitefly Bemisia tabaci biotype B. A DNA fragment of 1.2 kb was obtained by PCR with the primers PAL1v 1978 and PAR1c 496 for component A, and a DNA fragment of 0.5 kb was obtained with the primers PBL1v 2040 and PCR cl for component B, confirming the presence of a begomovirus infecting M. lathyroides. Molecular studies indicated that the begomovirus isolate showed 77% genomic similarity with Bean golden mosaic virus and 75% with Cowpea golden mosaic virus for their cp and rep genes, indicating the possibility that the isolate is a distinct virus species of the Begomovirus genus.http://www.redalyc.org/articulo.oa?id=237123825003 |
| spellingShingle | JOSÉ ALBERSIO DE ARAUJO LIMA ALINE KELLY QUEIROZ DO NASCIMENTO VANUZIA BATISTA OLIVEIRA AMORIM PAULA RADAELLI JOAQUIM TORRES FILHO SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides Revista Caatinga |
| title | SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides |
| title_full | SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides |
| title_fullStr | SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides |
| title_full_unstemmed | SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides |
| title_short | SEROLOGICAL DETECTION AND MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS ISOLATE OBTAINED FROM Macroptilium lathyroides |
| title_sort | serological detection and molecular characterization of a begomovirus isolate obtained from macroptilium lathyroides |
| url | http://www.redalyc.org/articulo.oa?id=237123825003 |
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