Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice
Adipose tissue (AT) is the primary reservoir of lipid, the major thermogenesis organ during cold exposure, and an important site for lactate production. However, the utilization of lactate as a metabolic substrate by adipocytes, as well as its potential involvement in the regulation of adipocyte the...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2072-6643/15/22/4846 |
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| author | Bin Guo Hui Shu Ling Luo Xiangpeng Liu Yue Ma Jie Zhang Zhiwei Liu Yong Zhang Lei Fu Tongxing Song Yixue Qiao Chi Zhang |
| author_facet | Bin Guo Hui Shu Ling Luo Xiangpeng Liu Yue Ma Jie Zhang Zhiwei Liu Yong Zhang Lei Fu Tongxing Song Yixue Qiao Chi Zhang |
| author_sort | Bin Guo |
| collection | DOAJ |
| description | Adipose tissue (AT) is the primary reservoir of lipid, the major thermogenesis organ during cold exposure, and an important site for lactate production. However, the utilization of lactate as a metabolic substrate by adipocytes, as well as its potential involvement in the regulation of adipocyte thermogenesis, remain unappreciated. In vitro experiments using primary stromal vascular fraction preadipocytes isolated from mouse inguinal white adipose tissue (iWAT) revealed that lactate dehydrogenase B (LDHB), the key glycolytic enzyme that catalyzes the conversion of lactate to pyruvate, is upregulated during adipocyte differentiation, downregulated upon chronic cold stimulation, and regained after prolonged cold exposure. In addition, the global knockout of <i>Ldhb</i> significantly reduced the masses of iWAT and epididymal WAT (eWAT) and impeded the utilization of iWAT during cold exposure. In addition, <i>Ldhb</i> loss of function impaired the mitochondrial function of iWAT under cold conditions. Together, these findings uncover the involvement of LDHB in adipocyte differentiation and thermogenesis. |
| first_indexed | 2024-03-09T16:33:14Z |
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| id | doaj.art-535e6c22d6324810ab6f05ba23472d1e |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T16:33:14Z |
| publishDate | 2023-11-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-535e6c22d6324810ab6f05ba23472d1e2023-11-24T14:59:32ZengMDPI AGNutrients2072-66432023-11-011522484610.3390/nu15224846Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in MiceBin Guo0Hui Shu1Ling Luo2Xiangpeng Liu3Yue Ma4Jie Zhang5Zhiwei Liu6Yong Zhang7Lei Fu8Tongxing Song9Yixue Qiao10Chi Zhang11The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital), Dongguan 523018, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaWisdom Lake Academy of Pharmacy, Xi’an Jiaotong-Liverpool University, Suzhou 215123, ChinaCollege of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaWisdom Lake Academy of Pharmacy, Xi’an Jiaotong-Liverpool University, Suzhou 215123, ChinaCambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, ChinaAdipose tissue (AT) is the primary reservoir of lipid, the major thermogenesis organ during cold exposure, and an important site for lactate production. However, the utilization of lactate as a metabolic substrate by adipocytes, as well as its potential involvement in the regulation of adipocyte thermogenesis, remain unappreciated. In vitro experiments using primary stromal vascular fraction preadipocytes isolated from mouse inguinal white adipose tissue (iWAT) revealed that lactate dehydrogenase B (LDHB), the key glycolytic enzyme that catalyzes the conversion of lactate to pyruvate, is upregulated during adipocyte differentiation, downregulated upon chronic cold stimulation, and regained after prolonged cold exposure. In addition, the global knockout of <i>Ldhb</i> significantly reduced the masses of iWAT and epididymal WAT (eWAT) and impeded the utilization of iWAT during cold exposure. In addition, <i>Ldhb</i> loss of function impaired the mitochondrial function of iWAT under cold conditions. Together, these findings uncover the involvement of LDHB in adipocyte differentiation and thermogenesis.https://www.mdpi.com/2072-6643/15/22/4846lactate dehydrogenase B (LDHB)beige adipocytethermogenesis |
| spellingShingle | Bin Guo Hui Shu Ling Luo Xiangpeng Liu Yue Ma Jie Zhang Zhiwei Liu Yong Zhang Lei Fu Tongxing Song Yixue Qiao Chi Zhang Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice Nutrients lactate dehydrogenase B (LDHB) beige adipocyte thermogenesis |
| title | Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice |
| title_full | Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice |
| title_fullStr | Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice |
| title_full_unstemmed | Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice |
| title_short | Lactate Conversion by Lactate Dehydrogenase B Is Involved in Beige Adipocyte Differentiation and Thermogenesis in Mice |
| title_sort | lactate conversion by lactate dehydrogenase b is involved in beige adipocyte differentiation and thermogenesis in mice |
| topic | lactate dehydrogenase B (LDHB) beige adipocyte thermogenesis |
| url | https://www.mdpi.com/2072-6643/15/22/4846 |
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