Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model
Background: Co-administration of streptozotocin and fructose is believed to induce type 2 diabetes as well as to cause reproductive toxicity and testicular damage via increasing oxidative stress in rats. Objectives: In this study, the potential protective effect of Costus afer leaves methanol ext...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Hamadan University of Medical Sciences
2021-12-01
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| Series: | Avicenna Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | http://ajmb.umsha.ac.ir/PDF/ajmb-9-72.pdf |
| _version_ | 1811294123943002112 |
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| author | Tope Gafar Atere Oluseyi Adeboye Akinloye Regina Ngozi Ugbaja David Ajiboye Ojo |
| author_facet | Tope Gafar Atere Oluseyi Adeboye Akinloye Regina Ngozi Ugbaja David Ajiboye Ojo |
| author_sort | Tope Gafar Atere |
| collection | DOAJ |
| description | Background: Co-administration of streptozotocin and fructose is believed to induce type 2 diabetes as well as to cause reproductive toxicity and testicular damage via increasing oxidative stress in rats.
Objectives: In this study, the potential protective effect of Costus afer leaves methanol extract (CAME) on andrological parameters and pituitary-gonadal axis hormones of type 2 diabetes (T2D) in rats treated with streptozotocin and fructose was investigated.
Methods: A total of 35 rats were divided into five groups, each including seven rats. Group 1 received normal saline, whereas T2D was induced in rats from groups 2, 3, 4, and 5. Group 2 served as diabetic control; while groups 3, 4, and 5 were treated orally with 12 mg/kg body weight (BW) of metformin as well as 100 and 200 BW of CAME, respectively, for 4 weeks. Hypothalamic–pituitary–gonadal responses, andrological parameters, DNA fragmentation, and oxidative stress parameters of the reproductive organs were examined in all treatment groups.
Results: Administration of CAME reduced the degenerative changes in testes, epididymis and improved pituitary-gonadal axis hormone concentrations, and sperm morphology occasioned by the treatments.
Conclusion: It was concluded that the administration of CAME ameliorated reproductive abnormalities in T2D rat models treated with streptozotocin-fructose administration. |
| first_indexed | 2024-04-13T05:11:59Z |
| format | Article |
| id | doaj.art-53616ac79c6948b3843d3adc64d23479 |
| institution | Directory Open Access Journal |
| issn | 2345-4113 |
| language | English |
| last_indexed | 2024-04-13T05:11:59Z |
| publishDate | 2021-12-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Medical Biochemistry |
| spelling | doaj.art-53616ac79c6948b3843d3adc64d234792022-12-22T03:01:00ZengHamadan University of Medical SciencesAvicenna Journal of Medical Biochemistry2345-41132021-12-0192728210.34172/ajmb.2021.13Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats ModelTope Gafar Atere0https://orcid.org/0000-0001-9349-0990Oluseyi Adeboye Akinloye1Regina Ngozi Ugbaja2David Ajiboye Ojo3Department of Medical Biochemistry, College of Health Sciences, Osun State University, Osogbo, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, Abeokuta, NigeriaDepartment of Biochemistry, College of Bioscience, Federal University of Agriculture, Abeokuta, NigeriaDepartment of Microbiology, College of Bioscience, Federal University of Agriculture, Abeokuta, NigeriaBackground: Co-administration of streptozotocin and fructose is believed to induce type 2 diabetes as well as to cause reproductive toxicity and testicular damage via increasing oxidative stress in rats. Objectives: In this study, the potential protective effect of Costus afer leaves methanol extract (CAME) on andrological parameters and pituitary-gonadal axis hormones of type 2 diabetes (T2D) in rats treated with streptozotocin and fructose was investigated. Methods: A total of 35 rats were divided into five groups, each including seven rats. Group 1 received normal saline, whereas T2D was induced in rats from groups 2, 3, 4, and 5. Group 2 served as diabetic control; while groups 3, 4, and 5 were treated orally with 12 mg/kg body weight (BW) of metformin as well as 100 and 200 BW of CAME, respectively, for 4 weeks. Hypothalamic–pituitary–gonadal responses, andrological parameters, DNA fragmentation, and oxidative stress parameters of the reproductive organs were examined in all treatment groups. Results: Administration of CAME reduced the degenerative changes in testes, epididymis and improved pituitary-gonadal axis hormone concentrations, and sperm morphology occasioned by the treatments. Conclusion: It was concluded that the administration of CAME ameliorated reproductive abnormalities in T2D rat models treated with streptozotocin-fructose administration.http://ajmb.umsha.ac.ir/PDF/ajmb-9-72.pdfcostus aferandrological parametersdiabetes mellitusoxidative stresssperm analysis |
| spellingShingle | Tope Gafar Atere Oluseyi Adeboye Akinloye Regina Ngozi Ugbaja David Ajiboye Ojo Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model Avicenna Journal of Medical Biochemistry costus afer andrological parameters diabetes mellitus oxidative stress sperm analysis |
| title | Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model |
| title_full | Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model |
| title_fullStr | Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model |
| title_full_unstemmed | Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model |
| title_short | Standardized Extract of Costus Afer Ker. Gawl leaves Modulates Reproductive Toxicity Caused by Fructose-Streptozotocin Administration in Type-2 Diabetic Rats Model |
| title_sort | standardized extract of costus afer ker gawl leaves modulates reproductive toxicity caused by fructose streptozotocin administration in type 2 diabetic rats model |
| topic | costus afer andrological parameters diabetes mellitus oxidative stress sperm analysis |
| url | http://ajmb.umsha.ac.ir/PDF/ajmb-9-72.pdf |
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