Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses
As a typical seasonal breeding animal, male muskrats have a pair of scent glands that can emit musky odor substances to attract females during the breeding period. The present study aimed to visualize the differences in the distribution of lipids in the scent glands of muskrats during their differen...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/14/2228 |
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| author | Wenqian Xie Shengheng Mu Jinkun Zhong Chaoran Zhang Haolin Zhang Xiaodong Wang Qiang Weng |
| author_facet | Wenqian Xie Shengheng Mu Jinkun Zhong Chaoran Zhang Haolin Zhang Xiaodong Wang Qiang Weng |
| author_sort | Wenqian Xie |
| collection | DOAJ |
| description | As a typical seasonal breeding animal, male muskrats have a pair of scent glands that can emit musky odor substances to attract females during the breeding period. The present study aimed to visualize the differences in the distribution of lipids in the scent glands of muskrats during their different reproductive statuses by imaging mass spectrometry and quantitative real-time PCR (qRT-PCR). The results revealed remarkable differences in the expression and spatial distribution of lipids detected in the scent glands of muskrats during the different reproductive statuses. In addition, the expression levels of lipid molecules PC (32:0) and LysoPC (16:0) were found to be significantly higher in the breeding season than in the non-breeding season. Moreover, the mRNA expression levels of lipid synthesis enzyme <i>Pemt</i> and <i>Pla2g4b</i> were higher in the breeding season than in the non-breeding season, and there were positive correlations between the expression intensities of lipid molecules and the expression levels of <i>Pemt</i> and <i>Pla2g4b</i>. The present study investigates the changes and distribution of the endogenous lipid in the scent glands of muskrats and elucidates that the seasonal changes in the lipid metabolism may affect the functions of the scent glands in muskrats. |
| first_indexed | 2024-03-09T12:05:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T12:05:35Z |
| publishDate | 2022-07-01 |
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| series | Cells |
| spelling | doaj.art-53617dde44984692b13c8b34cd92fdb72023-11-30T22:58:50ZengMDPI AGCells2073-44092022-07-011114222810.3390/cells11142228Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive StatusesWenqian Xie0Shengheng Mu1Jinkun Zhong2Chaoran Zhang3Haolin Zhang4Xiaodong Wang5Qiang Weng6Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Mass Spectrometry Imaging and Metabolomics, Minzu University of China, State Ethnic Affairs Commission, Beijing 100081, ChinaLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaAs a typical seasonal breeding animal, male muskrats have a pair of scent glands that can emit musky odor substances to attract females during the breeding period. The present study aimed to visualize the differences in the distribution of lipids in the scent glands of muskrats during their different reproductive statuses by imaging mass spectrometry and quantitative real-time PCR (qRT-PCR). The results revealed remarkable differences in the expression and spatial distribution of lipids detected in the scent glands of muskrats during the different reproductive statuses. In addition, the expression levels of lipid molecules PC (32:0) and LysoPC (16:0) were found to be significantly higher in the breeding season than in the non-breeding season. Moreover, the mRNA expression levels of lipid synthesis enzyme <i>Pemt</i> and <i>Pla2g4b</i> were higher in the breeding season than in the non-breeding season, and there were positive correlations between the expression intensities of lipid molecules and the expression levels of <i>Pemt</i> and <i>Pla2g4b</i>. The present study investigates the changes and distribution of the endogenous lipid in the scent glands of muskrats and elucidates that the seasonal changes in the lipid metabolism may affect the functions of the scent glands in muskrats.https://www.mdpi.com/2073-4409/11/14/2228lipid metabolismMALDI-MSIseasonal changesscent glandmuskrat |
| spellingShingle | Wenqian Xie Shengheng Mu Jinkun Zhong Chaoran Zhang Haolin Zhang Xiaodong Wang Qiang Weng Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses Cells lipid metabolism MALDI-MSI seasonal changes scent gland muskrat |
| title | Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses |
| title_full | Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses |
| title_fullStr | Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses |
| title_full_unstemmed | Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses |
| title_short | Mass Spectrometry Imaging of Lipids in the Scent Glands of Muskrat (<i>Ondatra zibethicus</i>) in Different Reproductive Statuses |
| title_sort | mass spectrometry imaging of lipids in the scent glands of muskrat i ondatra zibethicus i in different reproductive statuses |
| topic | lipid metabolism MALDI-MSI seasonal changes scent gland muskrat |
| url | https://www.mdpi.com/2073-4409/11/14/2228 |
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