Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss
<p>Abstract</p> <p>Allelic loss of chromosome 1p is frequently observed in oligodendroglioma. We screened 177 oligodendroglial tumors for 1p deletions and found 6 tumors with localized 1p36 deletions. Several apoptosis regulation genes have been mapped to this region, including <...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-09-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/4/1/35 |
| _version_ | 1811315396566843392 |
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| author | Fuller Gregory N Bruner Janet Sawaya Raymond Taylor Ellen Dunmire Valerie McDonald J Matthew Aldape Kenneth Zhang Wei |
| author_facet | Fuller Gregory N Bruner Janet Sawaya Raymond Taylor Ellen Dunmire Valerie McDonald J Matthew Aldape Kenneth Zhang Wei |
| author_sort | Fuller Gregory N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Allelic loss of chromosome 1p is frequently observed in oligodendroglioma. We screened 177 oligodendroglial tumors for 1p deletions and found 6 tumors with localized 1p36 deletions. Several apoptosis regulation genes have been mapped to this region, including <it>Tumor Protein 73 (p73)</it>, <it>DNA Fragmentation Factor subunits alpha </it>(<it>DFFA</it>) and <it>beta </it>(<it>DFFB</it>), and <it>Tumor Necrosis Factor Receptor Superfamily Members 9 </it>and <it>25 </it>(<it>TNFRSF9, TNFRSF25</it>). We compared expression levels of these 5 genes in pairs of 1p-loss and 1p-intact tumors using quantitative reverse-transcriptase PCR (QRTPCR) to test if 1p deletions had an effect on expression. Only the <it>DFFB </it>gene demonstrated decreased expression in all tumor pairs tested. Mutational analysis did not reveal <it>DFFB </it>mutations in 12 tested samples. However, it is possible that <it>DFFB </it>haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development.</p> |
| first_indexed | 2024-04-13T11:29:27Z |
| format | Article |
| id | doaj.art-53645d3b74c94ab3bd59ff8572b4f15e |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-13T11:29:27Z |
| publishDate | 2005-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-53645d3b74c94ab3bd59ff8572b4f15e2022-12-22T02:48:36ZengBMCMolecular Cancer1476-45982005-09-01413510.1186/1476-4598-4-35Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic LossFuller Gregory NBruner JanetSawaya RaymondTaylor EllenDunmire ValerieMcDonald J MatthewAldape KennethZhang Wei<p>Abstract</p> <p>Allelic loss of chromosome 1p is frequently observed in oligodendroglioma. We screened 177 oligodendroglial tumors for 1p deletions and found 6 tumors with localized 1p36 deletions. Several apoptosis regulation genes have been mapped to this region, including <it>Tumor Protein 73 (p73)</it>, <it>DNA Fragmentation Factor subunits alpha </it>(<it>DFFA</it>) and <it>beta </it>(<it>DFFB</it>), and <it>Tumor Necrosis Factor Receptor Superfamily Members 9 </it>and <it>25 </it>(<it>TNFRSF9, TNFRSF25</it>). We compared expression levels of these 5 genes in pairs of 1p-loss and 1p-intact tumors using quantitative reverse-transcriptase PCR (QRTPCR) to test if 1p deletions had an effect on expression. Only the <it>DFFB </it>gene demonstrated decreased expression in all tumor pairs tested. Mutational analysis did not reveal <it>DFFB </it>mutations in 12 tested samples. However, it is possible that <it>DFFB </it>haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development.</p>http://www.molecular-cancer.com/content/4/1/35 |
| spellingShingle | Fuller Gregory N Bruner Janet Sawaya Raymond Taylor Ellen Dunmire Valerie McDonald J Matthew Aldape Kenneth Zhang Wei Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss Molecular Cancer |
| title | Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss |
| title_full | Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss |
| title_fullStr | Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss |
| title_full_unstemmed | Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss |
| title_short | Attenuated Expression of <it>DFFB </it>is a Hallmark of Oligodendrogliomas with 1p-Allelic Loss |
| title_sort | attenuated expression of it dffb it is a hallmark of oligodendrogliomas with 1p allelic loss |
| url | http://www.molecular-cancer.com/content/4/1/35 |
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