Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker
Due to the environmental changes and habitat destruction the mudskipper fish population is decreasing in recent years. To predict the fish population structure, frequent manual survey and molecular methods are widely used. Molecular markers such as RAPD, microsatellite, allozyme, D-loop haplotype ar...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2013-05-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/8992 |
| _version_ | 1818171634140315648 |
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| author | Vellaichamy RAMANADEVI Muthusamy THANGARAJ Anbazhagan SURESHKUMAR Jayachandran SUBBURAJ |
| author_facet | Vellaichamy RAMANADEVI Muthusamy THANGARAJ Anbazhagan SURESHKUMAR Jayachandran SUBBURAJ |
| author_sort | Vellaichamy RAMANADEVI |
| collection | DOAJ |
| description | Due to the environmental changes and habitat destruction the mudskipper fish population is decreasing in recent years. To predict the fish population structure, frequent manual survey and molecular methods are widely used. Molecular markers such as RAPD, microsatellite, allozyme, D-loop haplotype are frequently adopted to assess the population structure of an organism. In this study ten- arbitrary primers were screened to estimate the genetic relationships and diversity of two mudskipper species (Boleophthalmus boddarti and B. dussumieri) in Vellar estuary, Tamilnadu, India. By this RAPD marker study, the genetic diversity (H) in B. boddarti was more (0.0116 ± 0.0066) than in B. dussumieri (0.0056 ± 0.0024) in Vellar estuary (India). The genetic distance between B. boddarti and B. dussumieri was 1.7943. By observing the species specific bands and the phylogenetic analysis it is revealed that these two species clearly deviated into separate clusters emphasizing the distinct species status. |
| first_indexed | 2024-12-11T18:59:50Z |
| format | Article |
| id | doaj.art-5366310319b74385affbfa4e1a34aa90 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-11T18:59:50Z |
| publishDate | 2013-05-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-5366310319b74385affbfa4e1a34aa902022-12-22T00:54:02ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642013-05-01521391437952Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD MarkerVellaichamy RAMANADEVI0Muthusamy THANGARAJ1Anbazhagan SURESHKUMAR2Jayachandran SUBBURAJ3Annamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil NaduAnnamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil NaduAnnamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil NaduAnnamalai University, Centre of Advanced Study in Marine Biology, 608 502 Parangipettai, Tamil NaduDue to the environmental changes and habitat destruction the mudskipper fish population is decreasing in recent years. To predict the fish population structure, frequent manual survey and molecular methods are widely used. Molecular markers such as RAPD, microsatellite, allozyme, D-loop haplotype are frequently adopted to assess the population structure of an organism. In this study ten- arbitrary primers were screened to estimate the genetic relationships and diversity of two mudskipper species (Boleophthalmus boddarti and B. dussumieri) in Vellar estuary, Tamilnadu, India. By this RAPD marker study, the genetic diversity (H) in B. boddarti was more (0.0116 ± 0.0066) than in B. dussumieri (0.0056 ± 0.0024) in Vellar estuary (India). The genetic distance between B. boddarti and B. dussumieri was 1.7943. By observing the species specific bands and the phylogenetic analysis it is revealed that these two species clearly deviated into separate clusters emphasizing the distinct species status.http://notulaebiologicae.ro/index.php/nsb/article/view/8992 |
| spellingShingle | Vellaichamy RAMANADEVI Muthusamy THANGARAJ Anbazhagan SURESHKUMAR Jayachandran SUBBURAJ Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker Notulae Scientia Biologicae |
| title | Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker |
| title_full | Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker |
| title_fullStr | Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker |
| title_full_unstemmed | Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker |
| title_short | Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker |
| title_sort | detection of genetic variation and genetic diversity in two indian mudskipper species boleophthalmus boddarti b dussumieri using rapd marker |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/8992 |
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