MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy
Abstract Background Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC. Methods We applied qRT-PCR, western blot and im...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-020-1095-4 |
| _version_ | 1818595629829455872 |
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| author | Li Zhang Xudong Liu Lina Song Hui Zhai Chaohua Chang |
| author_facet | Li Zhang Xudong Liu Lina Song Hui Zhai Chaohua Chang |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | Abstract Background Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC. Methods We applied qRT-PCR, western blot and immunochemistry to detect the expression difference between normal tissue and CC. In vitro, we establish MAP7 stable knocking down and overexpression cell lines and investigated the function and underlying mechanism of MAP7 in CC. Results Both mRNA and protein of MAP7 were upregulated in CC compared with the normal tissue. MAP7 was correlated with the clinical stage and tumor size and lymph node metastasis. MAP7 promotes the invasion and migration of CC cell lines. We next detected EMT pathway and autophagy associated pathway. MAP7 promotes the EMT through modulating the autophagy. Conclusion Taken above, our results showed that MAP7 promotes the migration and invasion and EMT through modulating the autophagy. |
| first_indexed | 2024-12-16T11:19:04Z |
| format | Article |
| id | doaj.art-5368859a05e24e94b742c7de442f7b32 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-16T11:19:04Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-5368859a05e24e94b742c7de442f7b322022-12-21T22:33:30ZengBMCCancer Cell International1475-28672020-01-012011810.1186/s12935-020-1095-4MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagyLi Zhang0Xudong Liu1Lina Song2Hui Zhai3Chaohua Chang4Department of Gynaecology and Obstetrics, Jinan Women and Children Health HospitalDepartment of Pain, Qilu Hospital of Shandong UniversityDepartment of Gynaecology and Obstetrics, Jinan Women and Children Health HospitalDepartment of Gynaecology and Obstetrics, Jinan Women and Children Health HospitalDepartment of Anethesia, Jinan Women and Children Health HospitalAbstract Background Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC. Methods We applied qRT-PCR, western blot and immunochemistry to detect the expression difference between normal tissue and CC. In vitro, we establish MAP7 stable knocking down and overexpression cell lines and investigated the function and underlying mechanism of MAP7 in CC. Results Both mRNA and protein of MAP7 were upregulated in CC compared with the normal tissue. MAP7 was correlated with the clinical stage and tumor size and lymph node metastasis. MAP7 promotes the invasion and migration of CC cell lines. We next detected EMT pathway and autophagy associated pathway. MAP7 promotes the EMT through modulating the autophagy. Conclusion Taken above, our results showed that MAP7 promotes the migration and invasion and EMT through modulating the autophagy.https://doi.org/10.1186/s12935-020-1095-4Cervical cancerMAP7Migration and invasionEpithelial-mesenchymal transition |
| spellingShingle | Li Zhang Xudong Liu Lina Song Hui Zhai Chaohua Chang MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy Cancer Cell International Cervical cancer MAP7 Migration and invasion Epithelial-mesenchymal transition |
| title | MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| title_full | MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| title_fullStr | MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| title_full_unstemmed | MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| title_short | MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| title_sort | map7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy |
| topic | Cervical cancer MAP7 Migration and invasion Epithelial-mesenchymal transition |
| url | https://doi.org/10.1186/s12935-020-1095-4 |
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