Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice
Background and Objective: Adult neurogenesis occurs in most mammalian species in two main areas of brain: 1- subventricular zone 2- the dentate gyrus of the hippocampus. Many factors such as 17-B estradiol affect neurogenesis in the hippocampus. The aim of this study was to investigate the effect of...
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| Format: | Article |
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Golestan University of Medical Sciences
2018-05-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1048&slc_lang=en&sid=1 |
| _version_ | 1819087928202100736 |
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| author | Maryam Azari Mohammad Taghi Ghorbanian Mahmoud Elah Dadi Salmani |
| author_facet | Maryam Azari Mohammad Taghi Ghorbanian Mahmoud Elah Dadi Salmani |
| author_sort | Maryam Azari |
| collection | DOAJ |
| description | Background and Objective: Adult neurogenesis occurs in most mammalian species in two main areas of brain: 1- subventricular zone 2- the dentate gyrus of the hippocampus. Many factors such as 17-B estradiol affect neurogenesis in the hippocampus. The aim of this study was to investigate the effect of exogenous 17-B estradiol on neurogenesis and astrocyte functions in the ovariectomized mice.
Methods: In this experimental study; NMRI mice were allocated into five experimental groups including Sham, Control, Treatment with single dose of 17-B estradiol two weeks after ovariectomy (OVX) and were sacrificed 24 hours later, Treatment with single dose of 17-B estradiol two weeks after Ovx and were sacrificed 48 hours later and Treatment with single dose of Seasame Oil 2 weeks after OVX and were sacrificed after 24 hours. Animals were transcardially perfused with paraformaldehyde. Brains were removed and its sections for cresyl fast violet staining and GFAP immunohistochemistry were prepared. Cells were counted and investigated.
Results: Neuronal density and Proliferation of hippocampal progenitor cells in the CA1 region of 17-B estradiol treated mice significantly increased up to 24 hours (P<0.05). Density of glia and particularly astrocytes in different regions of the hippocampus significantly reduced after treatment with 17-B estradiol (P<0.05).
Conclusion: Density of hippocampal CA1 neurons are influenced by 17-B estradiol. Also, density and morphology of glia cells, especially astrocytes in different regions of the hippocampus are affected by 17-B estradiol. |
| first_indexed | 2024-12-21T21:43:56Z |
| format | Article |
| id | doaj.art-5369347ee9f34927a6794824c5c61423 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-21T21:43:56Z |
| publishDate | 2018-05-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-5369347ee9f34927a6794824c5c614232022-12-21T18:49:18ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802018-05-012023541Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized miceMaryam Azari0Mohammad Taghi Ghorbanian1Mahmoud Elah Dadi Salmani2 M.Sc in Histology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran Assistant Professor, Depatment of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran. ghorbanian@du.ac.ir Associate Professor, Department of Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran Background and Objective: Adult neurogenesis occurs in most mammalian species in two main areas of brain: 1- subventricular zone 2- the dentate gyrus of the hippocampus. Many factors such as 17-B estradiol affect neurogenesis in the hippocampus. The aim of this study was to investigate the effect of exogenous 17-B estradiol on neurogenesis and astrocyte functions in the ovariectomized mice. Methods: In this experimental study; NMRI mice were allocated into five experimental groups including Sham, Control, Treatment with single dose of 17-B estradiol two weeks after ovariectomy (OVX) and were sacrificed 24 hours later, Treatment with single dose of 17-B estradiol two weeks after Ovx and were sacrificed 48 hours later and Treatment with single dose of Seasame Oil 2 weeks after OVX and were sacrificed after 24 hours. Animals were transcardially perfused with paraformaldehyde. Brains were removed and its sections for cresyl fast violet staining and GFAP immunohistochemistry were prepared. Cells were counted and investigated. Results: Neuronal density and Proliferation of hippocampal progenitor cells in the CA1 region of 17-B estradiol treated mice significantly increased up to 24 hours (P<0.05). Density of glia and particularly astrocytes in different regions of the hippocampus significantly reduced after treatment with 17-B estradiol (P<0.05). Conclusion: Density of hippocampal CA1 neurons are influenced by 17-B estradiol. Also, density and morphology of glia cells, especially astrocytes in different regions of the hippocampus are affected by 17-B estradiol.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1048&slc_lang=en&sid=117-B estradiolNeurogenesisHippocampusAstrocyteMouse |
| spellingShingle | Maryam Azari Mohammad Taghi Ghorbanian Mahmoud Elah Dadi Salmani Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice مجله دانشگاه علوم پزشکی گرگان 17-B estradiol Neurogenesis Hippocampus Astrocyte Mouse |
| title | Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| title_full | Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| title_fullStr | Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| title_full_unstemmed | Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| title_short | Effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| title_sort | effect of exogenous estrogen treatment on hippocampal neurogenesis in ovariectomized mice |
| topic | 17-B estradiol Neurogenesis Hippocampus Astrocyte Mouse |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1048&slc_lang=en&sid=1 |
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