CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury
Background Interactions between the heterotopic ossification (HO) and the neurological injuries have got lots of interest but still poorly understood. The present study aims to determine the effect of neuroinflammation related calcitonin gene-related protein (CGRP) on HO following spinal cord injury...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626865 |
| _version_ | 1818008789910028288 |
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| author | Xiguang Sang Zhiyong Wang Ping Shi Yonggang Li Lin Cheng |
| author_facet | Xiguang Sang Zhiyong Wang Ping Shi Yonggang Li Lin Cheng |
| author_sort | Xiguang Sang |
| collection | DOAJ |
| description | Background Interactions between the heterotopic ossification (HO) and the neurological injuries have got lots of interest but still poorly understood. The present study aims to determine the effect of neuroinflammation related calcitonin gene-related protein (CGRP) on HO following spinal cord injury (SCI).Method C57Bl6 mice were anesthetized to make an SCI model, together with the ectopic injection of snake venom cardiotoxin (SVC) or CGRP protein into tibialis anterior (TA) muscles. The TA muscles were then harvested for radiologic, histologic, and immunohistology examinations to determine the formation of bone and the expression of CGRP. Fibro/adipogenic progenitors (FAPs) were isolated and cultured. CGRP protein was added into the medium and then detected for chondrogenic markers.Result The callus formed following SVC injection and SCI successfully, with much more formed in the group with SCI, as well as an increased level of CGRP. Similarly, the injection of CGRP directly was able to induce the formation of bone-like tissues in vivo. And, the additional CGRP protein was able to induce chondrogenic differentiation as shown in RT-PCT and immunofluorescence, in a dose-dependent manner.Conclusion CGRP was able to induce chondrogenic differentiation of FAPs, which may be an important part of Neurological heterotopic ossification developing. |
| first_indexed | 2024-04-14T05:33:45Z |
| format | Article |
| id | doaj.art-536ba60910384a87b54cc3a424a4210b |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-14T05:33:45Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-536ba60910384a87b54cc3a424a4210b2022-12-22T02:09:43ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712569257410.1080/21691401.2019.1626865CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injuryXiguang Sang0Zhiyong Wang1Ping Shi2Yonggang Li3Lin Cheng4Department of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, P. R. ChinaDepartment of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, P. R. ChinaDepartment of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, P. R. ChinaDepartment of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, P. R. ChinaDepartment of Emergency Surgery, Qilu Hospital of Shandong University, Jinan, P. R. ChinaBackground Interactions between the heterotopic ossification (HO) and the neurological injuries have got lots of interest but still poorly understood. The present study aims to determine the effect of neuroinflammation related calcitonin gene-related protein (CGRP) on HO following spinal cord injury (SCI).Method C57Bl6 mice were anesthetized to make an SCI model, together with the ectopic injection of snake venom cardiotoxin (SVC) or CGRP protein into tibialis anterior (TA) muscles. The TA muscles were then harvested for radiologic, histologic, and immunohistology examinations to determine the formation of bone and the expression of CGRP. Fibro/adipogenic progenitors (FAPs) were isolated and cultured. CGRP protein was added into the medium and then detected for chondrogenic markers.Result The callus formed following SVC injection and SCI successfully, with much more formed in the group with SCI, as well as an increased level of CGRP. Similarly, the injection of CGRP directly was able to induce the formation of bone-like tissues in vivo. And, the additional CGRP protein was able to induce chondrogenic differentiation as shown in RT-PCT and immunofluorescence, in a dose-dependent manner.Conclusion CGRP was able to induce chondrogenic differentiation of FAPs, which may be an important part of Neurological heterotopic ossification developing.https://www.tandfonline.com/doi/10.1080/21691401.2019.1626865CGRPneurological heterotopic ossificationspinal cord injurychondrogenic differentiation |
| spellingShingle | Xiguang Sang Zhiyong Wang Ping Shi Yonggang Li Lin Cheng CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury Artificial Cells, Nanomedicine, and Biotechnology CGRP neurological heterotopic ossification spinal cord injury chondrogenic differentiation |
| title | CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| title_full | CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| title_fullStr | CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| title_full_unstemmed | CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| title_short | CGRP accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| title_sort | cgrp accelerates the pathogenesis of neurological heterotopic ossification following spinal cord injury |
| topic | CGRP neurological heterotopic ossification spinal cord injury chondrogenic differentiation |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1626865 |
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