Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases
The early detection of Parkinson’s disease (PD) is a critical issue in terms of efficiency. Alpha-synuclein (α-Syn) is a biomarker in PD checks. Alpha-synuclein (α-syn) is the major constituent of Lewy bodies and a pathogenic hallmark of all synucleinopathies, including PDs, dementia with Lewy bodie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/911 |
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| author | Hossein Navay Baghban Mohammad Hasanzadeh Yuqian Liu Farzad Seidi |
| author_facet | Hossein Navay Baghban Mohammad Hasanzadeh Yuqian Liu Farzad Seidi |
| author_sort | Hossein Navay Baghban |
| collection | DOAJ |
| description | The early detection of Parkinson’s disease (PD) is a critical issue in terms of efficiency. Alpha-synuclein (α-Syn) is a biomarker in PD checks. Alpha-synuclein (α-syn) is the major constituent of Lewy bodies and a pathogenic hallmark of all synucleinopathies, including PDs, dementia with Lewy bodies, and multiple system atrophy. In this study, KCC-1-NH-CS<sub>2</sub> was conjugated with biotinylated Ab and entrapped in P(β-CD) polymer cavities. Using this approach, a novel electrochemical label-free immunosensor was designed for the quantification of α-syn in real human samples. For this purpose, the glassy carbon electrode electropolymerized with P(β-CD) biopolymer provided an excellent matrix for entrapping of KCC-1-NH-CS<sub>2</sub> loaded with the biotinylated antibody of α-syn. Using the chronoamperometric technique, the proposed immunosensor shows a suitable range of 0.02 to 64 ng/mL for the determination of α-syn. Additionally, a low limit of quantification of the engineered biosensor was obtained at 0.02 ng/mL. The developed immunosensor’s adequate stability, sensitivity, and selectivity, together with its ease of manufacture, make it a promising diagnostic technique for further research. This study also will pave the way for further applications of the synergetic effect of β-CD and KCC-1-NH-CS<sub>2</sub> for biomedical analysis in the near future. |
| first_indexed | 2024-03-09T20:34:53Z |
| format | Article |
| id | doaj.art-536e57661f494f5fadf36f63adccf1cc |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:34:53Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-536e57661f494f5fadf36f63adccf1cc2023-11-23T23:12:39ZengMDPI AGBiosensors2079-63742022-10-01121091110.3390/bios12100911Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative DiseasesHossein Navay Baghban0Mohammad Hasanzadeh1Yuqian Liu2Farzad Seidi3Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, ChinaPharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranJiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, ChinaJiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources and International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, ChinaThe early detection of Parkinson’s disease (PD) is a critical issue in terms of efficiency. Alpha-synuclein (α-Syn) is a biomarker in PD checks. Alpha-synuclein (α-syn) is the major constituent of Lewy bodies and a pathogenic hallmark of all synucleinopathies, including PDs, dementia with Lewy bodies, and multiple system atrophy. In this study, KCC-1-NH-CS<sub>2</sub> was conjugated with biotinylated Ab and entrapped in P(β-CD) polymer cavities. Using this approach, a novel electrochemical label-free immunosensor was designed for the quantification of α-syn in real human samples. For this purpose, the glassy carbon electrode electropolymerized with P(β-CD) biopolymer provided an excellent matrix for entrapping of KCC-1-NH-CS<sub>2</sub> loaded with the biotinylated antibody of α-syn. Using the chronoamperometric technique, the proposed immunosensor shows a suitable range of 0.02 to 64 ng/mL for the determination of α-syn. Additionally, a low limit of quantification of the engineered biosensor was obtained at 0.02 ng/mL. The developed immunosensor’s adequate stability, sensitivity, and selectivity, together with its ease of manufacture, make it a promising diagnostic technique for further research. This study also will pave the way for further applications of the synergetic effect of β-CD and KCC-1-NH-CS<sub>2</sub> for biomedical analysis in the near future.https://www.mdpi.com/2079-6374/12/10/911immunosensoralpha-synucleinencapsulationbiomedical analysisceramic materialimmunocomplex |
| spellingShingle | Hossein Navay Baghban Mohammad Hasanzadeh Yuqian Liu Farzad Seidi Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases Biosensors immunosensor alpha-synuclein encapsulation biomedical analysis ceramic material immunocomplex |
| title | Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases |
| title_full | Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases |
| title_fullStr | Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases |
| title_full_unstemmed | Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases |
| title_short | Efficient Entrapment of Alpha-Synuclein Biotinylated Antibody in KCC-1-NH-CS<sub>2</sub> and Application for the Sensitive Diagnosis of Parkinson’s Using Recognition of Biomarker: An Innovative Electrochemical Label-Free Immunosensor for the Biomedical Analysis of Neurodegenerative Diseases |
| title_sort | efficient entrapment of alpha synuclein biotinylated antibody in kcc 1 nh cs sub 2 sub and application for the sensitive diagnosis of parkinson s using recognition of biomarker an innovative electrochemical label free immunosensor for the biomedical analysis of neurodegenerative diseases |
| topic | immunosensor alpha-synuclein encapsulation biomedical analysis ceramic material immunocomplex |
| url | https://www.mdpi.com/2079-6374/12/10/911 |
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