Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China
Objective: There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resis...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S141386701630023X |
| _version_ | 1831671198401953792 |
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| author | Youyun Zhao Jianhua Wu Lijun Sun Guangzhong Liu Bo Li Yi Zheng Xiaodong Li Junxiu Tao |
| author_facet | Youyun Zhao Jianhua Wu Lijun Sun Guangzhong Liu Bo Li Yi Zheng Xiaodong Li Junxiu Tao |
| author_sort | Youyun Zhao |
| collection | DOAJ |
| description | Objective: There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resistance mutations in Central China. Methods: This study included treatment-naive patients with CHB from June 2012 to May 2015 receiving care at the Institute of Liver Disease in Central China. All patients completed a questionnaire covering different aspects, such as family medical history, course of liver disease, medication history, alcohol use, among others. Mutations in HBV DNA polymerase associated with NAs resistance were detected using INNO-LiPA assay. Results: 269 patients were infected with HBV genotype B (81.4%), C (17.9%), and both B and C (0.7%). Mutations in HBV DNA polymerase were detected in 24 patients (8.9%) including rtM204I/V (n = 6), rtN236T (n = 5), rtM250V (n = 2), rtL180M (n = 2), rtT184G (n = 1), rtM207I (n = 1), rtS202I (n = 1), rtM204V/I & rtL180M (n = 5), and rtM204I & rtM250V (n = 1). Conclusion: Spontaneous HBV resistance mutations in HBV DNA polymerase were found in treatment-naive patients with CHB in Central China. These findings suggest that we should analyze HBV DNA polymerase resistance mutation associated with NAs before giving antiviral therapy such as lamivudine (LAM), adefovir (ADV), and telbivudine (LdT). Keywords: Hepatitis B virus, Chronic hepatitis B, Viral resistance mutations |
| first_indexed | 2024-12-19T23:32:05Z |
| format | Article |
| id | doaj.art-536f71aeac48414ba9a69a65860af49a |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-19T23:32:05Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-536f71aeac48414ba9a69a65860af49a2022-12-21T20:01:42ZengElsevierBrazilian Journal of Infectious Diseases1413-86702016-03-01202173178S1413-86702016000200173Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central ChinaYouyun Zhao0Jianhua Wu1Lijun Sun2Guangzhong Liu3Bo Li4Yi Zheng5Xiaodong Li6Junxiu Tao7Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China; Department of Clinical Laboratory, Hubei University of Traditional Chinese Medicine, Wuhan, China; Corresponding authors.Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China; Corresponding authors.Hubei Center for Clinical Laboratory, Wuhan 430064, China; Corresponding authors.Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaDepartment of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaDepartment of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaInstitute of Liver Disease, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaInstitute of Liver Disease, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, ChinaObjective: There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resistance mutations in Central China. Methods: This study included treatment-naive patients with CHB from June 2012 to May 2015 receiving care at the Institute of Liver Disease in Central China. All patients completed a questionnaire covering different aspects, such as family medical history, course of liver disease, medication history, alcohol use, among others. Mutations in HBV DNA polymerase associated with NAs resistance were detected using INNO-LiPA assay. Results: 269 patients were infected with HBV genotype B (81.4%), C (17.9%), and both B and C (0.7%). Mutations in HBV DNA polymerase were detected in 24 patients (8.9%) including rtM204I/V (n = 6), rtN236T (n = 5), rtM250V (n = 2), rtL180M (n = 2), rtT184G (n = 1), rtM207I (n = 1), rtS202I (n = 1), rtM204V/I & rtL180M (n = 5), and rtM204I & rtM250V (n = 1). Conclusion: Spontaneous HBV resistance mutations in HBV DNA polymerase were found in treatment-naive patients with CHB in Central China. These findings suggest that we should analyze HBV DNA polymerase resistance mutation associated with NAs before giving antiviral therapy such as lamivudine (LAM), adefovir (ADV), and telbivudine (LdT). Keywords: Hepatitis B virus, Chronic hepatitis B, Viral resistance mutationshttp://www.sciencedirect.com/science/article/pii/S141386701630023X |
| spellingShingle | Youyun Zhao Jianhua Wu Lijun Sun Guangzhong Liu Bo Li Yi Zheng Xiaodong Li Junxiu Tao Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China Brazilian Journal of Infectious Diseases |
| title | Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China |
| title_full | Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China |
| title_fullStr | Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China |
| title_full_unstemmed | Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China |
| title_short | Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China |
| title_sort | prevalence of mutations in hbv dna polymerase gene associated with nucleos t ide resistance in treatment naive patients with chronic hepatitis b in central china |
| url | http://www.sciencedirect.com/science/article/pii/S141386701630023X |
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