A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific
Teleost fish are the most diverse group of vertebrates and provide opportunities to study the evolution of sex determination (SD) systems. Using genomic and functional analyses, we identified a male-specific duplication of anti-Müllerian hormone (amh) gene as the male master sex-determining (MSD) ge...
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| Format: | Article |
| Language: | English |
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The Royal Society
2021-07-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.210063 |
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| author | Weihao Song Yuheng Xie Minmin Sun Xuemei Li Cristín K. Fitzpatrick Felix Vaux Kathleen G. O'Malley Quanqi Zhang Jie Qi Yan He |
| author_facet | Weihao Song Yuheng Xie Minmin Sun Xuemei Li Cristín K. Fitzpatrick Felix Vaux Kathleen G. O'Malley Quanqi Zhang Jie Qi Yan He |
| author_sort | Weihao Song |
| collection | DOAJ |
| description | Teleost fish are the most diverse group of vertebrates and provide opportunities to study the evolution of sex determination (SD) systems. Using genomic and functional analyses, we identified a male-specific duplication of anti-Müllerian hormone (amh) gene as the male master sex-determining (MSD) gene in Sebastes schlegelii. By resequencing 10 males and 10 females, we characterized a 5 kb-long fragment in HiC_Scaffold_12 as a male-specific region, which contained an amh gene (named amhy). We then demonstrated that amhy is a duplication of autosomal amh that was later translocated to the ancestral Y chromosome. amha and amhy shared high-nucleotide identity with the most significant difference being two insertions in intron 4 of amhy. Furthermore, amhy overexpression triggered female-to-male sex reversal in S. schlegelii, displaying its fundamental role in driving testis differentiation. We developed a PCR assay which successfully identified sexes in two species of northwest Pacific rockfish related to S. schlegelii. However, the PCR assay failed to distinguish the sexes in a separate clade of northeast Pacific rockfish. Our study provides new examples of amh as the MSD in fish and sheds light on the convergent evolution of amh duplication as the driving force of sex determination in different fish taxa. |
| first_indexed | 2024-04-11T13:34:12Z |
| format | Article |
| id | doaj.art-53700cc37daf4f24816a77ce77678366 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-04-11T13:34:12Z |
| publishDate | 2021-07-01 |
| publisher | The Royal Society |
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| series | Open Biology |
| spelling | doaj.art-53700cc37daf4f24816a77ce776783662022-12-22T04:21:36ZengThe Royal SocietyOpen Biology2046-24412021-07-0111710.1098/rsob.210063A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest PacificWeihao Song0Yuheng Xie1Minmin Sun2Xuemei Li3Cristín K. Fitzpatrick4Felix Vaux5Kathleen G. O'Malley6Quanqi Zhang7Jie Qi8Yan He9MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USAMOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaMOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaState Fisheries Genomics Lab, Coastal Oregon Marine Experiment Station, Department of Fisheries and Wildlife, Hatfield Marine Science Center, Oregon State University, Newport, OR, USADepartment of Zoology, University of Otago, Dunedin, New ZealandState Fisheries Genomics Lab, Coastal Oregon Marine Experiment Station, Department of Fisheries and Wildlife, Hatfield Marine Science Center, Oregon State University, Newport, OR, USAMOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaMOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaMOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of ChinaTeleost fish are the most diverse group of vertebrates and provide opportunities to study the evolution of sex determination (SD) systems. Using genomic and functional analyses, we identified a male-specific duplication of anti-Müllerian hormone (amh) gene as the male master sex-determining (MSD) gene in Sebastes schlegelii. By resequencing 10 males and 10 females, we characterized a 5 kb-long fragment in HiC_Scaffold_12 as a male-specific region, which contained an amh gene (named amhy). We then demonstrated that amhy is a duplication of autosomal amh that was later translocated to the ancestral Y chromosome. amha and amhy shared high-nucleotide identity with the most significant difference being two insertions in intron 4 of amhy. Furthermore, amhy overexpression triggered female-to-male sex reversal in S. schlegelii, displaying its fundamental role in driving testis differentiation. We developed a PCR assay which successfully identified sexes in two species of northwest Pacific rockfish related to S. schlegelii. However, the PCR assay failed to distinguish the sexes in a separate clade of northeast Pacific rockfish. Our study provides new examples of amh as the MSD in fish and sheds light on the convergent evolution of amh duplication as the driving force of sex determination in different fish taxa.https://royalsocietypublishing.org/doi/10.1098/rsob.210063sex determinationamhsex markergene duplicationSebastes rockfishesresequencing |
| spellingShingle | Weihao Song Yuheng Xie Minmin Sun Xuemei Li Cristín K. Fitzpatrick Felix Vaux Kathleen G. O'Malley Quanqi Zhang Jie Qi Yan He A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific Open Biology sex determination amh sex marker gene duplication Sebastes rockfishes resequencing |
| title | A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific |
| title_full | A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific |
| title_fullStr | A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific |
| title_full_unstemmed | A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific |
| title_short | A duplicated amh is the master sex-determining gene for Sebastes rockfish in the Northwest Pacific |
| title_sort | duplicated amh is the master sex determining gene for sebastes rockfish in the northwest pacific |
| topic | sex determination amh sex marker gene duplication Sebastes rockfishes resequencing |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.210063 |
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