Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model
Objective(s): Cisplatin (CP), as an anti-neoplastic drug, causes testicular damage. Zataria multiflora Boiss (ZM), a medicinal plant, has antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effects of ZM against CP-induced testicular toxicity. Materials and Meth...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-06-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_10672_ce661192c8bf5ff34b2385ad384a79f0.pdf |
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| author | Shokooh Karimi Seyed Jalal Hosseinimehr Hamid Reza Mohammadi Ali Reza Khalatbary Fereshteh Talebpour Amiri |
| author_facet | Shokooh Karimi Seyed Jalal Hosseinimehr Hamid Reza Mohammadi Ali Reza Khalatbary Fereshteh Talebpour Amiri |
| author_sort | Shokooh Karimi |
| collection | DOAJ |
| description | Objective(s): Cisplatin (CP), as an anti-neoplastic drug, causes testicular damage. Zataria multiflora Boiss (ZM), a medicinal plant, has antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effects of ZM against CP-induced testicular toxicity. Materials and Methods: In this experimental study, thirty-two adult male mice were randomly divided into four groups. The control group received normal saline with oral gavage during 7 days; ZM group received ZM (200 mg/kg) during 7 days by gavage; CP group received CP (10 mg/kg) intraperitoneally (IP) in the 5th day of study; ZM + CP group received ZM during 7 days and CP was injected in 5th day. Sperm parameters, biochemical (MDA, GSH, and PC) levels, serum testosterone levels, and histopathological and immunohistochemical assays of testis were examined one day after the last drug treatment. Results: CP treatment caused significant damage via changed sperm parameters (sperm motility, count, viability rate, and abnormalities), increased oxidative stress (increased MDA and PC levels, and decreased GSH level), histological changes (degeneration, necrosis, arrest of spermatogenesis, congestion, and decrease in thickness of the germinal epithelium, diameter of seminiferous tubules, and Johnsen’s Score), decreased serum testosterone level, and increased caspase-3 immunoreactivity. ZM preserved spermatogenesis and mitigated the toxic effects of CP on the testis tissue. In addition, treatment with ZM significantly reduced caspase-3 immunoreactivity. Conclusion: The findings of this study suggest that ZM as a potential antioxidant compound and due to free radicals scavenging activities has a protective effect against CP-induced testicular toxicity. |
| first_indexed | 2024-12-20T05:55:32Z |
| format | Article |
| id | doaj.art-53712eb92c4645f49f452038a5615f71 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-20T05:55:32Z |
| publishDate | 2018-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-53712eb92c4645f49f452038a5615f712022-12-21T19:51:04ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-06-0121660761410.22038/ijbms.2018.26784.655810672Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice modelShokooh Karimi0Seyed Jalal Hosseinimehr1Hamid Reza Mohammadi2Ali Reza Khalatbary3Fereshteh Talebpour Amiri4Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranDepartment of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranDepartment of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranDepartment of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, IranObjective(s): Cisplatin (CP), as an anti-neoplastic drug, causes testicular damage. Zataria multiflora Boiss (ZM), a medicinal plant, has antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effects of ZM against CP-induced testicular toxicity. Materials and Methods: In this experimental study, thirty-two adult male mice were randomly divided into four groups. The control group received normal saline with oral gavage during 7 days; ZM group received ZM (200 mg/kg) during 7 days by gavage; CP group received CP (10 mg/kg) intraperitoneally (IP) in the 5th day of study; ZM + CP group received ZM during 7 days and CP was injected in 5th day. Sperm parameters, biochemical (MDA, GSH, and PC) levels, serum testosterone levels, and histopathological and immunohistochemical assays of testis were examined one day after the last drug treatment. Results: CP treatment caused significant damage via changed sperm parameters (sperm motility, count, viability rate, and abnormalities), increased oxidative stress (increased MDA and PC levels, and decreased GSH level), histological changes (degeneration, necrosis, arrest of spermatogenesis, congestion, and decrease in thickness of the germinal epithelium, diameter of seminiferous tubules, and Johnsen’s Score), decreased serum testosterone level, and increased caspase-3 immunoreactivity. ZM preserved spermatogenesis and mitigated the toxic effects of CP on the testis tissue. In addition, treatment with ZM significantly reduced caspase-3 immunoreactivity. Conclusion: The findings of this study suggest that ZM as a potential antioxidant compound and due to free radicals scavenging activities has a protective effect against CP-induced testicular toxicity.http://ijbms.mums.ac.ir/article_10672_ce661192c8bf5ff34b2385ad384a79f0.pdfCaspase-3CisplatinOxidative stressTesticular toxicityZataria multiflora |
| spellingShingle | Shokooh Karimi Seyed Jalal Hosseinimehr Hamid Reza Mohammadi Ali Reza Khalatbary Fereshteh Talebpour Amiri Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model Iranian Journal of Basic Medical Sciences Caspase-3 Cisplatin Oxidative stress Testicular toxicity Zataria multiflora |
| title | Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| title_full | Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| title_fullStr | Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| title_full_unstemmed | Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| title_short | Zataria multiflora ameliorates cisplatin-induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| title_sort | zataria multiflora ameliorates cisplatin induced testicular damage via suppression of oxidative stress and apoptosis in a mice model |
| topic | Caspase-3 Cisplatin Oxidative stress Testicular toxicity Zataria multiflora |
| url | http://ijbms.mums.ac.ir/article_10672_ce661192c8bf5ff34b2385ad384a79f0.pdf |
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