A Rationally Designed Bovine IgA Fc Scaffold Enhances in planta Accumulation of a VHH-Fc Fusion Without Compromising Binding to Enterohemorrhagic E. coli

A Rationally Designed Bovine IgA Fc Scaffold Enhances in planta Accumulation of a VHH-Fc Fusion Without Compromising Binding to Enterohemorrhagic E. coli

We previously isolated a single domain antibody (VHH) that binds Enterohemorrhagic Escherichia coli (EHEC) with the end-goal being the enteromucosal passive immunization of cattle herds. To improve the yield of a chimeric fusion of the VHH with an IgA Fc, we employed two rational design strategies,...

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Bibliographic Details
Main Authors: Adam Chin-Fatt, Reza Saberianfar, Rima Menassa
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Plant Science
Subjects:
enterohemorrhagic E. coli-EHEC
IgA
VHH antibody fragment
single domain antibody (sdAb)
Fc fusion
plant-made antibodies
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.651262/full

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