Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties
Optical diagnostics methods are significantly appealing in biological applications since they are non-destructive, safe, and minimally invasive. Laser-induced fluorescence is a promising optical spectrochemical analytical technique widely employed for tissue classification through molecular analysis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/11/2846 |
| _version_ | 1797465570940026880 |
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| author | Omnia Hamdy Zienab Abdel-Salam Mohamed Abdel-Harith |
| author_facet | Omnia Hamdy Zienab Abdel-Salam Mohamed Abdel-Harith |
| author_sort | Omnia Hamdy |
| collection | DOAJ |
| description | Optical diagnostics methods are significantly appealing in biological applications since they are non-destructive, safe, and minimally invasive. Laser-induced fluorescence is a promising optical spectrochemical analytical technique widely employed for tissue classification through molecular analysis of the studied samples after excitation with appropriate short-wavelength laser light. On the other hand, diffuse optics techniques are used for tissue monitoring and differentiation based on their absorption and scattering characteristics in the red to the near-infrared spectra. Therefore, it is strongly foreseen to obtain promising results by combining these techniques. In the present work, tissues under different conditions (hydrated/dry skin and native/boiled adipose fat) were distinguished according to their fluorescence emission, absorption, and scattering properties. The selected tissues’ optical absorption and scattering parameters were determined via Kubelka–Munk mathematical model according to the experimental tissue reflectance and transmittance measurements. Such measurements were obtained using an optical configuration of integrating sphere and spectrometer at different laser wavelengths (808, 830, and 980 nm). Moreover, the diffusion equation was solved for the fluence rate at the sample surface using the finite element method. Furthermore, the accuracy of the obtained spectroscopic measurements was evaluated using partial least squares regression statistical analysis with 0.87 and 0.89 R-squared values for skin and adipose fat, respectively. |
| first_indexed | 2024-03-09T18:23:20Z |
| format | Article |
| id | doaj.art-537587a6d5f74f49896022912f630941 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T18:23:20Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-537587a6d5f74f49896022912f6309412023-11-24T08:05:12ZengMDPI AGDiagnostics2075-44182022-11-011211284610.3390/diagnostics12112846Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering PropertiesOmnia Hamdy0Zienab Abdel-Salam1Mohamed Abdel-Harith2Engineering Applications of Lasers Department, National Institute of Laser Enhanced Sciences, Cairo University, Giza 12613, EgyptLaser Applications in Metrology, Photochemistry and Agriculture Department, National Institute of Laser Enhanced Sciences, Cairo University, Giza 12613, EgyptLaser Applications in Metrology, Photochemistry and Agriculture Department, National Institute of Laser Enhanced Sciences, Cairo University, Giza 12613, EgyptOptical diagnostics methods are significantly appealing in biological applications since they are non-destructive, safe, and minimally invasive. Laser-induced fluorescence is a promising optical spectrochemical analytical technique widely employed for tissue classification through molecular analysis of the studied samples after excitation with appropriate short-wavelength laser light. On the other hand, diffuse optics techniques are used for tissue monitoring and differentiation based on their absorption and scattering characteristics in the red to the near-infrared spectra. Therefore, it is strongly foreseen to obtain promising results by combining these techniques. In the present work, tissues under different conditions (hydrated/dry skin and native/boiled adipose fat) were distinguished according to their fluorescence emission, absorption, and scattering properties. The selected tissues’ optical absorption and scattering parameters were determined via Kubelka–Munk mathematical model according to the experimental tissue reflectance and transmittance measurements. Such measurements were obtained using an optical configuration of integrating sphere and spectrometer at different laser wavelengths (808, 830, and 980 nm). Moreover, the diffusion equation was solved for the fluence rate at the sample surface using the finite element method. Furthermore, the accuracy of the obtained spectroscopic measurements was evaluated using partial least squares regression statistical analysis with 0.87 and 0.89 R-squared values for skin and adipose fat, respectively.https://www.mdpi.com/2075-4418/12/11/2846laserbiological tissue monitoringfluorescenceabsorptionscattering |
| spellingShingle | Omnia Hamdy Zienab Abdel-Salam Mohamed Abdel-Harith Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties Diagnostics laser biological tissue monitoring fluorescence absorption scattering |
| title | Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties |
| title_full | Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties |
| title_fullStr | Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties |
| title_full_unstemmed | Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties |
| title_short | Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties |
| title_sort | optical characterization of biological tissues based on fluorescence absorption and scattering properties |
| topic | laser biological tissue monitoring fluorescence absorption scattering |
| url | https://www.mdpi.com/2075-4418/12/11/2846 |
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