Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema
Background:. Lymphedema is an intractable disease for which there is currently no established curative therapy. A reliable and long-lasting lymphedema model is essential for development of better treatments. In this study, we aimed to establish a simple, reproducible and long-lasting mouse model of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2023-09-01
|
| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005243 |
| _version_ | 1827805103326756864 |
|---|---|
| author | Yuichi Morita, MD Naoaki Sakata, MD Ryo Kawakami, MSc Masayuki Shimizu, MD Gumpei Yoshimatsu, MD Hideichi Wada, MD Shohta Kodama, MD |
| author_facet | Yuichi Morita, MD Naoaki Sakata, MD Ryo Kawakami, MSc Masayuki Shimizu, MD Gumpei Yoshimatsu, MD Hideichi Wada, MD Shohta Kodama, MD |
| author_sort | Yuichi Morita, MD |
| collection | DOAJ |
| description | Background:. Lymphedema is an intractable disease for which there is currently no established curative therapy. A reliable and long-lasting lymphedema model is essential for development of better treatments. In this study, we aimed to establish a simple, reproducible and long-lasting mouse model of lymphedema.
Methods:. Our model is characterized by a combination of a circumferential skin incision in the femoral region, complete dissection of regional lymph nodes, and ablation of the inguinal route in the femoral region. The characteristics of the lymphedema were evaluated and compared with those of two other models. One of these models involved dissection of the subiliac, popliteal, and sciatic lymph nodes (model A) and the other excision of the subiliac, popliteal, and sciatic lymph nodes with cauterization of lymphatic vessels and closure without a skin excision (model B).
Results:. Although the lymphedema in models A and B resolved spontaneously, that in the new model lasted for a month with increases in femoral circumference and hind limb volume, thickening of the skin, especially subcutaneous tissue, and congestion of peripheral lymphatic vessels. Furthermore, this model could be used for assessing the therapeutic effects of syngeneic mesenchymal stem cell transplantation. The average operation time for the new model was 14.4 ± 1.3 minutes.
Conclusion:. Long-lasting lymphedema can be achieved by our new model, making it suitable for assessing therapies for lymphedema. |
| first_indexed | 2024-03-11T21:20:49Z |
| format | Article |
| id | doaj.art-53783bba09714057a51b65b5718742e5 |
| institution | Directory Open Access Journal |
| issn | 2169-7574 |
| language | English |
| last_indexed | 2024-03-11T21:20:49Z |
| publishDate | 2023-09-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj.art-53783bba09714057a51b65b5718742e52023-09-28T07:09:02ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742023-09-01119e524310.1097/GOX.0000000000005243202309000-00012Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of LymphedemaYuichi Morita, MD0Naoaki Sakata, MD1Ryo Kawakami, MSc2Masayuki Shimizu, MD3Gumpei Yoshimatsu, MD4Hideichi Wada, MD5Shohta Kodama, MD6From the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, JapanFrom the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, JapanFrom the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, JapanFrom the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, JapanFrom the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, Japan‡ Department of Cardiovascular Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, JapanFrom the * Department of Regenerative Medicine & Transplantation, Faculty of Medicine, Fukuoka University, Fukuoka, JapanBackground:. Lymphedema is an intractable disease for which there is currently no established curative therapy. A reliable and long-lasting lymphedema model is essential for development of better treatments. In this study, we aimed to establish a simple, reproducible and long-lasting mouse model of lymphedema. Methods:. Our model is characterized by a combination of a circumferential skin incision in the femoral region, complete dissection of regional lymph nodes, and ablation of the inguinal route in the femoral region. The characteristics of the lymphedema were evaluated and compared with those of two other models. One of these models involved dissection of the subiliac, popliteal, and sciatic lymph nodes (model A) and the other excision of the subiliac, popliteal, and sciatic lymph nodes with cauterization of lymphatic vessels and closure without a skin excision (model B). Results:. Although the lymphedema in models A and B resolved spontaneously, that in the new model lasted for a month with increases in femoral circumference and hind limb volume, thickening of the skin, especially subcutaneous tissue, and congestion of peripheral lymphatic vessels. Furthermore, this model could be used for assessing the therapeutic effects of syngeneic mesenchymal stem cell transplantation. The average operation time for the new model was 14.4 ± 1.3 minutes. Conclusion:. Long-lasting lymphedema can be achieved by our new model, making it suitable for assessing therapies for lymphedema.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005243 |
| spellingShingle | Yuichi Morita, MD Naoaki Sakata, MD Ryo Kawakami, MSc Masayuki Shimizu, MD Gumpei Yoshimatsu, MD Hideichi Wada, MD Shohta Kodama, MD Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema Plastic and Reconstructive Surgery, Global Open |
| title | Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema |
| title_full | Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema |
| title_fullStr | Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema |
| title_full_unstemmed | Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema |
| title_short | Establishment of a Simple, Reproducible, and Long-lasting Hind Limb Animal Model of Lymphedema |
| title_sort | establishment of a simple reproducible and long lasting hind limb animal model of lymphedema |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000005243 |
| work_keys_str_mv | AT yuichimoritamd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT naoakisakatamd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT ryokawakamimsc establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT masayukishimizumd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT gumpeiyoshimatsumd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT hideichiwadamd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema AT shohtakodamamd establishmentofasimplereproducibleandlonglastinghindlimbanimalmodeloflymphedema |