Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells
UVB radiation can damage human skin, whereas Ginsenoside Rg3, the active ingredient in red ginseng that is processed from ginseng (Panax ginseng C.A. Meyer), could inhibit UVB induced cell damage through anti-oxidation. Meanwhile, P407/CS/HA hydrogel has significant biomedical applications as carrie...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S075333222300968X |
| _version_ | 1797744058441924608 |
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| author | Chuanbo Ding Xiaojuan Peng Jiali Yang Kecheng Chen Xinglong Liu Yingchun Zhao Shuai Zhang Shuwen Sun Jinping Zhang Qiteng Ding Shuang Liu Wencong Liu |
| author_facet | Chuanbo Ding Xiaojuan Peng Jiali Yang Kecheng Chen Xinglong Liu Yingchun Zhao Shuai Zhang Shuwen Sun Jinping Zhang Qiteng Ding Shuang Liu Wencong Liu |
| author_sort | Chuanbo Ding |
| collection | DOAJ |
| description | UVB radiation can damage human skin, whereas Ginsenoside Rg3, the active ingredient in red ginseng that is processed from ginseng (Panax ginseng C.A. Meyer), could inhibit UVB induced cell damage through anti-oxidation. Meanwhile, P407/CS/HA hydrogel has significant biomedical applications as carriers of drugs. However, the beneficial effects of Rg3-loaded hydrogel (Rg3-Gel) on human HaCaT keratinocytes induced by UVB have rarely been reported. In our study, Rg3 was loaded into hydrogel and the effect of Rg3-Gel against UVB‑induced Hacat cells damages was determined by measuring its ability to alleviate UVB‑induced elevation of oxidative stress, pro-inflammatory and apoptotic response. We found that the treatment with Rg3-Gel inhibited the generation of intracellular ROS and MDA and upregulated the expression of antioxidant enzymes SOD and GSH-Px which were inhibited by UVB exposure. Increased levels of pro-inflammatory cytokines TNF‑α, COX‑2, iNOS and IL‑1β following UVB irradiation were suppressed by the introduction of Rg3-Gel. Additionally, the level of Bcl-2 was decreased and the expression of Bax and Caspase3 were enhanced by Rg3-Gel treatment. In conclusion, Rg3-Gel equipped with the synergistic effect of Rg3 and hydrogel has an effective inhibitory effect on UVB-induced oxidative stress, inflammatory and apoptosis. |
| first_indexed | 2024-03-12T15:04:17Z |
| format | Article |
| id | doaj.art-5379aad6bc204555aa5b3bdad2f1aec3 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-12T15:04:17Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-5379aad6bc204555aa5b3bdad2f1aec32023-08-13T04:52:53ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-09-01165115177Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cellsChuanbo Ding0Xiaojuan Peng1Jiali Yang2Kecheng Chen3Xinglong Liu4Yingchun Zhao5Shuai Zhang6Shuwen Sun7Jinping Zhang8Qiteng Ding9Shuang Liu10Wencong Liu11Jilin Agricultural Science and Technology College, Jilin, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaStarsky Medical Research Center, Siping, Jilin 136001, ChinaJilin Agricultural Science and Technology College, Jilin, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaJilin Agricultural University, Changchun, Jilin 130118, ChinaGoldenwell Biotech, Inc, 50 West Liberty Street, Suite 880, Reno, NV 89501, USA; Corresponding authors.Jilin Agricultural University, Changchun, Jilin 130118, China; Corresponding authors.UVB radiation can damage human skin, whereas Ginsenoside Rg3, the active ingredient in red ginseng that is processed from ginseng (Panax ginseng C.A. Meyer), could inhibit UVB induced cell damage through anti-oxidation. Meanwhile, P407/CS/HA hydrogel has significant biomedical applications as carriers of drugs. However, the beneficial effects of Rg3-loaded hydrogel (Rg3-Gel) on human HaCaT keratinocytes induced by UVB have rarely been reported. In our study, Rg3 was loaded into hydrogel and the effect of Rg3-Gel against UVB‑induced Hacat cells damages was determined by measuring its ability to alleviate UVB‑induced elevation of oxidative stress, pro-inflammatory and apoptotic response. We found that the treatment with Rg3-Gel inhibited the generation of intracellular ROS and MDA and upregulated the expression of antioxidant enzymes SOD and GSH-Px which were inhibited by UVB exposure. Increased levels of pro-inflammatory cytokines TNF‑α, COX‑2, iNOS and IL‑1β following UVB irradiation were suppressed by the introduction of Rg3-Gel. Additionally, the level of Bcl-2 was decreased and the expression of Bax and Caspase3 were enhanced by Rg3-Gel treatment. In conclusion, Rg3-Gel equipped with the synergistic effect of Rg3 and hydrogel has an effective inhibitory effect on UVB-induced oxidative stress, inflammatory and apoptosis.http://www.sciencedirect.com/science/article/pii/S075333222300968XRg3-loaded hydrogelUVBHaCaT cellsAnti-oxidantAnti-inflammatoryAnti-apoptosis |
| spellingShingle | Chuanbo Ding Xiaojuan Peng Jiali Yang Kecheng Chen Xinglong Liu Yingchun Zhao Shuai Zhang Shuwen Sun Jinping Zhang Qiteng Ding Shuang Liu Wencong Liu Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells Biomedicine & Pharmacotherapy Rg3-loaded hydrogel UVB HaCaT cells Anti-oxidant Anti-inflammatory Anti-apoptosis |
| title | Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells |
| title_full | Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells |
| title_fullStr | Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells |
| title_full_unstemmed | Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells |
| title_short | Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells |
| title_sort | rg3 loaded p407 cs ha hydrogel inhibits uvb induced oxidative stress inflammation and apoptosis in hacat cells |
| topic | Rg3-loaded hydrogel UVB HaCaT cells Anti-oxidant Anti-inflammatory Anti-apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S075333222300968X |
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