Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin
The oxidative stress phenomenon is a result of anticancer therapy. The aim of this study was the assessment of gene expression profile changes, and to determine the miRNAs regulating genes’ transcriptional activity in an Ishikawa endometrial cancer culture exposed to cisplatin or salinomycin, compar...
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MDPI AG
2022-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/5/1190 |
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| author | Szymon Januszyk Paweł Mieszczański Hubert Lurka Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek |
| author_facet | Szymon Januszyk Paweł Mieszczański Hubert Lurka Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek |
| author_sort | Szymon Januszyk |
| collection | DOAJ |
| description | The oxidative stress phenomenon is a result of anticancer therapy. The aim of this study was the assessment of gene expression profile changes, and to determine the miRNAs regulating genes’ transcriptional activity in an Ishikawa endometrial cancer culture exposed to cisplatin or salinomycin, compared to a control culture. The molecular analysis comprised the microarray technique (mRNAs and micro RNA (miRNA), the real-time quantitative reverse transcription reaction (RTqPCR), enzyme-linked immunosorbent assay (ELISA) reactions, and Western blot. NR4A2, MAP3K8, ICAM1, IL21, CXCL8, CCL7, and SLC7A11 were statistically significantly differentiated depending not only on time, but also on the drug used in the experiment. The conducted assessment indicated that the strongest links were between NR4A2 and hsa-miR-30a-5p and has-miR-302e, MAP3K8 and hsa-miR-144-3p, CXCL8 and hsa-miR-140-3p, and SLC7A11 and hsa-miR-144-3p. The obtained results suggest that four mRNAs—NR4A2, MAP3K8, CXCL8 and SLC7A11—and four miRNAs—hsa-miR-30a-5p, hsa-miR-302e, hsa-miR-144-3p and hsa-miR-140-3—changed their expressions regardless of the chemotherapeutic agent used, which suggests the possibility of their use in monitoring the severity of oxidative stress in endometrial cancer. However, considering the results at both the mRNA and the protein level, it is most likely that the expressions of NR4A2, MAP3K8, CXCL8 and SLC7A11 are regulated by miRNA molecules as well as other epigenetic mechanisms. |
| first_indexed | 2024-03-10T03:16:27Z |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:16:27Z |
| publishDate | 2022-05-01 |
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| series | Biomedicines |
| spelling | doaj.art-5379bdb2dcac400fa4ca9575623ce0a62023-11-23T10:12:22ZengMDPI AGBiomedicines2227-90592022-05-01105119010.3390/biomedicines10051190Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or SalinomycinSzymon Januszyk0Paweł Mieszczański1Hubert Lurka2Dorota Sagan3Dariusz Boroń4Beniamin Oskar Grabarek5ICZ Healthcare Hospital in Zywiec, 34-300 Zywiec, PolandHospital of Ministry of Interior and Administration, 40-052 Katowice, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academia of Silesia in Katowice, 41-800 Zabrze, PolandMedical Center Dormed Medical SPA, 28-105 Busko-Zdroj, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academia of Silesia in Katowice, 41-800 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academia of Silesia in Katowice, 41-800 Zabrze, PolandThe oxidative stress phenomenon is a result of anticancer therapy. The aim of this study was the assessment of gene expression profile changes, and to determine the miRNAs regulating genes’ transcriptional activity in an Ishikawa endometrial cancer culture exposed to cisplatin or salinomycin, compared to a control culture. The molecular analysis comprised the microarray technique (mRNAs and micro RNA (miRNA), the real-time quantitative reverse transcription reaction (RTqPCR), enzyme-linked immunosorbent assay (ELISA) reactions, and Western blot. NR4A2, MAP3K8, ICAM1, IL21, CXCL8, CCL7, and SLC7A11 were statistically significantly differentiated depending not only on time, but also on the drug used in the experiment. The conducted assessment indicated that the strongest links were between NR4A2 and hsa-miR-30a-5p and has-miR-302e, MAP3K8 and hsa-miR-144-3p, CXCL8 and hsa-miR-140-3p, and SLC7A11 and hsa-miR-144-3p. The obtained results suggest that four mRNAs—NR4A2, MAP3K8, CXCL8 and SLC7A11—and four miRNAs—hsa-miR-30a-5p, hsa-miR-302e, hsa-miR-144-3p and hsa-miR-140-3—changed their expressions regardless of the chemotherapeutic agent used, which suggests the possibility of their use in monitoring the severity of oxidative stress in endometrial cancer. However, considering the results at both the mRNA and the protein level, it is most likely that the expressions of NR4A2, MAP3K8, CXCL8 and SLC7A11 are regulated by miRNA molecules as well as other epigenetic mechanisms.https://www.mdpi.com/2227-9059/10/5/1190cisplatinsalinomycinoxidative stressmRNAmiRNAcell line |
| spellingShingle | Szymon Januszyk Paweł Mieszczański Hubert Lurka Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin Biomedicines cisplatin salinomycin oxidative stress mRNA miRNA cell line |
| title | Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin |
| title_full | Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin |
| title_fullStr | Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin |
| title_full_unstemmed | Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin |
| title_short | Expression Profile of mRNAs and miRNAs Related to the Oxidative-Stress Phenomenon in the Ishikawa Cell Line Treated Either Cisplatin or Salinomycin |
| title_sort | expression profile of mrnas and mirnas related to the oxidative stress phenomenon in the ishikawa cell line treated either cisplatin or salinomycin |
| topic | cisplatin salinomycin oxidative stress mRNA miRNA cell line |
| url | https://www.mdpi.com/2227-9059/10/5/1190 |
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