Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age
ABSTRACT: Growth and response to Escherichia coli lipopolysaccharide (LPS) challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids (n-3 FA) and yeast bioactives (YB) from hatch through to 16 wk of age (woa) were investigated. Co-extruded full fat flaxseed and pulse mixture (FF...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123004595 |
| _version_ | 1797676347157381120 |
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| author | Junhyung Lee Veronica Cheng Elijah G. Kiarie |
| author_facet | Junhyung Lee Veronica Cheng Elijah G. Kiarie |
| author_sort | Junhyung Lee |
| collection | DOAJ |
| description | ABSTRACT: Growth and response to Escherichia coli lipopolysaccharide (LPS) challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids (n-3 FA) and yeast bioactives (YB) from hatch through to 16 wk of age (woa) were investigated. Co-extruded full fat flaxseed and pulse mixture (FFF; 1:1 wt/wt) supplied n-3 FA and YB were yeast cell walls processed with β-1,3-glucan hydrolase. A total of 1,064-day-old pullets were placed in cages (19 birds/cage) and allocated to 7 diets (n = 8). The iso-caloric and iso-nitrogenous diets were control, control + 1, 3, or 5% FFF and + 0.025, 0.05, or 0.1% YB. The birds had ad libitum access to feed and water. Body weight (BW), feed intake, and lymphoid organs weight were recorded. At 15 woa, 2 pairs of pullets/cage received intravenous injection of either 1 mL of sterile saline without or with 8 mg LPS/kg BW. Injected pullets were bled, monitored for BW and cloaca temperature at time points within 168 h. Birds fed 1% FFF were heavier (P < 0.04) than birds fed other diets at 16 woa with FFF and YB exhibiting non-linear responses. Control birds had heavier (P = 0.02) thymus at 4 woa. Spleen weight increased quadratically (P < 0.05) in response to FFF at 8 and YB at 16 woa. The LPS increased cloaca temperature and altered concentration of several plasma metabolites (P < 0.05). The interaction (P < 0.05) between LPS and diet was such that control birds exhibited lower creatine kinase (CK) upon challenge with LPS relative to birds fed other diets. The LPS birds fed 1% FFF and 0.05% YB showed higher plasma albumin than non-LPS cohorts. Non-LPS birds fed control, 1 and 3% FFF had higher plasma K than LPS cohorts. In general, FFF and YB exhibited linear and quadratic effects (P < 0.05) on select plasma metabolites. In conclusion, dietary provision of n-3 FA and YB influenced pullet BW at sexual maturity, development of lymphoid organs and modulated some plasma metabolites in response to LPS. |
| first_indexed | 2024-03-11T22:26:46Z |
| format | Article |
| id | doaj.art-537b0c9f43fa42f7a12078fa9370d22f |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-11T22:26:46Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj.art-537b0c9f43fa42f7a12078fa9370d22f2023-09-24T05:13:54ZengElsevierPoultry Science0032-57912023-10-0110210102940Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of ageJunhyung Lee0Veronica Cheng1Elijah G. Kiarie2University of Guelph, Department of Animal Biosciences, Guelph, ON, Canada, N1G 2W1University of Guelph, Department of Animal Biosciences, Guelph, ON, Canada, N1G 2W1Corresponding author:; University of Guelph, Department of Animal Biosciences, Guelph, ON, Canada, N1G 2W1ABSTRACT: Growth and response to Escherichia coli lipopolysaccharide (LPS) challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids (n-3 FA) and yeast bioactives (YB) from hatch through to 16 wk of age (woa) were investigated. Co-extruded full fat flaxseed and pulse mixture (FFF; 1:1 wt/wt) supplied n-3 FA and YB were yeast cell walls processed with β-1,3-glucan hydrolase. A total of 1,064-day-old pullets were placed in cages (19 birds/cage) and allocated to 7 diets (n = 8). The iso-caloric and iso-nitrogenous diets were control, control + 1, 3, or 5% FFF and + 0.025, 0.05, or 0.1% YB. The birds had ad libitum access to feed and water. Body weight (BW), feed intake, and lymphoid organs weight were recorded. At 15 woa, 2 pairs of pullets/cage received intravenous injection of either 1 mL of sterile saline without or with 8 mg LPS/kg BW. Injected pullets were bled, monitored for BW and cloaca temperature at time points within 168 h. Birds fed 1% FFF were heavier (P < 0.04) than birds fed other diets at 16 woa with FFF and YB exhibiting non-linear responses. Control birds had heavier (P = 0.02) thymus at 4 woa. Spleen weight increased quadratically (P < 0.05) in response to FFF at 8 and YB at 16 woa. The LPS increased cloaca temperature and altered concentration of several plasma metabolites (P < 0.05). The interaction (P < 0.05) between LPS and diet was such that control birds exhibited lower creatine kinase (CK) upon challenge with LPS relative to birds fed other diets. The LPS birds fed 1% FFF and 0.05% YB showed higher plasma albumin than non-LPS cohorts. Non-LPS birds fed control, 1 and 3% FFF had higher plasma K than LPS cohorts. In general, FFF and YB exhibited linear and quadratic effects (P < 0.05) on select plasma metabolites. In conclusion, dietary provision of n-3 FA and YB influenced pullet BW at sexual maturity, development of lymphoid organs and modulated some plasma metabolites in response to LPS.http://www.sciencedirect.com/science/article/pii/S0032579123004595omega-3 fatty acidsyeast bioactivespullet growth and developmentimmunocompetence |
| spellingShingle | Junhyung Lee Veronica Cheng Elijah G. Kiarie Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age Poultry Science omega-3 fatty acids yeast bioactives pullet growth and development immunocompetence |
| title | Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| title_full | Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| title_fullStr | Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| title_full_unstemmed | Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| title_short | Growth and response to Escherichia coli lipopolysaccharide challenge in Lohmann LSL-Lite pullets when fed a source of omega-3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| title_sort | growth and response to escherichia coli lipopolysaccharide challenge in lohmann lsl lite pullets when fed a source of omega 3 fatty acids and yeast bioactives from hatch through to 16 wk of age |
| topic | omega-3 fatty acids yeast bioactives pullet growth and development immunocompetence |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123004595 |
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