Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products

Legumes are valuable sources of proteins and other functional components. However, the high starch content can be an impediment in developing new vegan food formulations. Enzyme-assisted hydrolysis was used to hydrolyze the starch from chickpea and broad bean vegetable milk to further develop vegeta...

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Main Authors: Ina Vasilean, Iuliana Aprodu, Maria Garnai, Valeriu Munteanu, Livia Patrașcu
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/10/7/1530
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author Ina Vasilean
Iuliana Aprodu
Maria Garnai
Valeriu Munteanu
Livia Patrașcu
author_facet Ina Vasilean
Iuliana Aprodu
Maria Garnai
Valeriu Munteanu
Livia Patrașcu
author_sort Ina Vasilean
collection DOAJ
description Legumes are valuable sources of proteins and other functional components. However, the high starch content can be an impediment in developing new vegan food formulations. Enzyme-assisted hydrolysis was used to hydrolyze the starch from chickpea and broad bean vegetable milk to further develop vegetable lactic acid-fermented products. The antioxidant activity of legumes was tested, and it was observed that the overall antioxidant activity (DPPH radical scavenging ability) significantly increased after enzyme-assisted hydrolysis while total phenols content decreased. The obtained vegetable milk was then fermented using exopolysaccharides-producing lactic acid bacteria. A significant decolorization was observed after fermentation in the case of broad bean-based products. Rheological behavior of the fermented products was determined using small amplitude oscillatory measurements and the three-interval thixotropy test. Results showed higher complex viscosity values for broad bean-based products, which displayed a weak gel-like structure. The starter cultures used for vegetable milk samples fermentation influenced the resistance to flow.
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spelling doaj.art-537d244625f04731bc22071657a7a64f2023-11-22T03:46:44ZengMDPI AGFoods2304-81582021-07-01107153010.3390/foods10071530Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable ProductsIna Vasilean0Iuliana Aprodu1Maria Garnai2Valeriu Munteanu3Livia Patrașcu4Faculty of Food Science and Engineering, Dunarea de Jos University of Galati, 111 Domneasca Str, 800008 Galati, RomaniaFaculty of Food Science and Engineering, Dunarea de Jos University of Galati, 111 Domneasca Str, 800008 Galati, RomaniaFaculty of Food Science and Engineering, Dunarea de Jos University of Galati, 111 Domneasca Str, 800008 Galati, RomaniaCross-Border Faculty, Dunarea de Jos University of Galati, 111 Domneasca Str, 800008 Galati, RomaniaCross-Border Faculty, Dunarea de Jos University of Galati, 111 Domneasca Str, 800008 Galati, RomaniaLegumes are valuable sources of proteins and other functional components. However, the high starch content can be an impediment in developing new vegan food formulations. Enzyme-assisted hydrolysis was used to hydrolyze the starch from chickpea and broad bean vegetable milk to further develop vegetable lactic acid-fermented products. The antioxidant activity of legumes was tested, and it was observed that the overall antioxidant activity (DPPH radical scavenging ability) significantly increased after enzyme-assisted hydrolysis while total phenols content decreased. The obtained vegetable milk was then fermented using exopolysaccharides-producing lactic acid bacteria. A significant decolorization was observed after fermentation in the case of broad bean-based products. Rheological behavior of the fermented products was determined using small amplitude oscillatory measurements and the three-interval thixotropy test. Results showed higher complex viscosity values for broad bean-based products, which displayed a weak gel-like structure. The starter cultures used for vegetable milk samples fermentation influenced the resistance to flow.https://www.mdpi.com/2304-8158/10/7/1530enzyme hydrolysisvegetable milk substitutesrheological behaviorthree-interval thixotropy testantioxidant activity
spellingShingle Ina Vasilean
Iuliana Aprodu
Maria Garnai
Valeriu Munteanu
Livia Patrașcu
Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
Foods
enzyme hydrolysis
vegetable milk substitutes
rheological behavior
three-interval thixotropy test
antioxidant activity
title Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
title_full Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
title_fullStr Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
title_full_unstemmed Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
title_short Preliminary Investigations into the Use of Amylases and Lactic Acid Bacteria to Obtain Fermented Vegetable Products
title_sort preliminary investigations into the use of amylases and lactic acid bacteria to obtain fermented vegetable products
topic enzyme hydrolysis
vegetable milk substitutes
rheological behavior
three-interval thixotropy test
antioxidant activity
url https://www.mdpi.com/2304-8158/10/7/1530
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