Opto-magnetic capture of individual cells based on visual phenotypes
The ability to isolate rare live cells within a heterogeneous population based solely on visual criteria remains technically challenging, due largely to limitations imposed by existing sorting technologies. Here, we present a new method that permits labeling cells of interest by attaching streptavid...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/45239 |
| _version_ | 1811200030291263488 |
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| author | Loïc Binan François Bélanger Maxime Uriarte Jean François Lemay Jean Christophe Pelletier De Koninck Joannie Roy El Bachir Affar Elliot Drobetsky Hugo Wurtele Santiago Costantino |
| author_facet | Loïc Binan François Bélanger Maxime Uriarte Jean François Lemay Jean Christophe Pelletier De Koninck Joannie Roy El Bachir Affar Elliot Drobetsky Hugo Wurtele Santiago Costantino |
| author_sort | Loïc Binan |
| collection | DOAJ |
| description | The ability to isolate rare live cells within a heterogeneous population based solely on visual criteria remains technically challenging, due largely to limitations imposed by existing sorting technologies. Here, we present a new method that permits labeling cells of interest by attaching streptavidin-coated magnetic beads to their membranes using the lasers of a confocal microscope. A simple magnet allows highly specific isolation of the labeled cells, which then remain viable and proliferate normally. As proof of principle, we tagged, isolated, and expanded individual cells based on three biologically relevant visual characteristics: i) presence of multiple nuclei, ii) accumulation of lipid vesicles, and iii) ability to resolve ionizing radiation-induced DNA damage foci. Our method constitutes a rapid, efficient, and cost-effective approach for isolation and subsequent characterization of rare cells based on observable traits such as movement, shape, or location, which in turn can generate novel mechanistic insights into important biological processes. |
| first_indexed | 2024-04-12T01:57:57Z |
| format | Article |
| id | doaj.art-537e4cd9b73c45eb9749faa532d5e2a4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:57:57Z |
| publishDate | 2019-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-537e4cd9b73c45eb9749faa532d5e2a42022-12-22T03:52:45ZengeLife Sciences Publications LtdeLife2050-084X2019-04-01810.7554/eLife.45239Opto-magnetic capture of individual cells based on visual phenotypesLoïc Binan0François Bélanger1Maxime Uriarte2Jean François Lemay3https://orcid.org/0000-0002-3540-1627Jean Christophe Pelletier De Koninck4Joannie Roy5El Bachir Affar6Elliot Drobetsky7Hugo Wurtele8Santiago Costantino9https://orcid.org/0000-0002-2454-2635Research Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Ophthalmology, University of Montreal, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Medicine and Molecular Biology Program, University of Montreal, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Medicine and Molecular Biology Program, University of Montreal, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Medicine and Molecular Biology Program, University of Montreal, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Medicine and Molecular Biology Program, University of Montreal, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, CanadaResearch Center, Maisonneuve-Rosemont Hospital, Montreal, Canada; Department of Ophthalmology, University of Montreal, Montreal, CanadaThe ability to isolate rare live cells within a heterogeneous population based solely on visual criteria remains technically challenging, due largely to limitations imposed by existing sorting technologies. Here, we present a new method that permits labeling cells of interest by attaching streptavidin-coated magnetic beads to their membranes using the lasers of a confocal microscope. A simple magnet allows highly specific isolation of the labeled cells, which then remain viable and proliferate normally. As proof of principle, we tagged, isolated, and expanded individual cells based on three biologically relevant visual characteristics: i) presence of multiple nuclei, ii) accumulation of lipid vesicles, and iii) ability to resolve ionizing radiation-induced DNA damage foci. Our method constitutes a rapid, efficient, and cost-effective approach for isolation and subsequent characterization of rare cells based on observable traits such as movement, shape, or location, which in turn can generate novel mechanistic insights into important biological processes.https://elifesciences.org/articles/45239single cell capturevisual phenotypescell sortingrare cellsclonal expansioncell labeling |
| spellingShingle | Loïc Binan François Bélanger Maxime Uriarte Jean François Lemay Jean Christophe Pelletier De Koninck Joannie Roy El Bachir Affar Elliot Drobetsky Hugo Wurtele Santiago Costantino Opto-magnetic capture of individual cells based on visual phenotypes eLife single cell capture visual phenotypes cell sorting rare cells clonal expansion cell labeling |
| title | Opto-magnetic capture of individual cells based on visual phenotypes |
| title_full | Opto-magnetic capture of individual cells based on visual phenotypes |
| title_fullStr | Opto-magnetic capture of individual cells based on visual phenotypes |
| title_full_unstemmed | Opto-magnetic capture of individual cells based on visual phenotypes |
| title_short | Opto-magnetic capture of individual cells based on visual phenotypes |
| title_sort | opto magnetic capture of individual cells based on visual phenotypes |
| topic | single cell capture visual phenotypes cell sorting rare cells clonal expansion cell labeling |
| url | https://elifesciences.org/articles/45239 |
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