Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy
Abstract Controlling the selective one‐to‐one conjugation of RNA with nanoparticles is vital for future applications of RNA nanotechnology. Here, the monofunctionalization of a gold nanoparticle (AuNP) with a single copy of RNA is developed for ultrasensitive microRNA‐155 quantification using electr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-02-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.201902477 |
| _version_ | 1818939989030862848 |
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| author | Taek Lee Mohsen Mohammadniaei Hui Zhang Jinho Yoon Hye Kyu Choi Sijin Guo Peixuan Guo Jeong‐Woo Choi |
| author_facet | Taek Lee Mohsen Mohammadniaei Hui Zhang Jinho Yoon Hye Kyu Choi Sijin Guo Peixuan Guo Jeong‐Woo Choi |
| author_sort | Taek Lee |
| collection | DOAJ |
| description | Abstract Controlling the selective one‐to‐one conjugation of RNA with nanoparticles is vital for future applications of RNA nanotechnology. Here, the monofunctionalization of a gold nanoparticle (AuNP) with a single copy of RNA is developed for ultrasensitive microRNA‐155 quantification using electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS). A single AuNP is conjugated with one copy of the packaging RNA (pRNA) three‐way junction (RNA 3WJ). pRNA 3WJ containing one strand of the 3WJ is connected to a Sephadex G100 aptamer and a biotin group at each arm (SEPapt/3WJ/Bio) which is then immobilized to the Sephadex G100 resin. The resulting complex is connected to streptavidin‐coated AuNP (STV/AuNP). Next, the STV/AuNP–Bio/3WJa is purified and reassembled with another 3WJ to form a single‐labeled 3WJ/AuNP. Later, the monoconjugate is immobilized onto the AuNP‐electrodeposited indium tin oxide coated substrate for detecting microRNA‐155 based on EC‐SERS. Application of an optimum potential of +0.2 V results in extraordinary amplification (≈7 times) of methylene blue (reporter) SERS signal compared to the normal SERS signal. As a result, a highly sensitive detection of 60 × 10−18 m microRNA‐155 in 1 h in serum based on monoconjugated AuNP/RNA is achieved. Thus, the monofunctionalization of RNA onto nanoparticle can provide a new methodology for biosensor construction and diverse RNA nanotechnology development. |
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| id | doaj.art-5381f0d4f7dd48469e705317a683e0b4 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-20T06:32:30Z |
| publishDate | 2020-02-01 |
| publisher | Wiley |
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| series | Advanced Science |
| spelling | doaj.art-5381f0d4f7dd48469e705317a683e0b42022-12-21T19:50:06ZengWileyAdvanced Science2198-38442020-02-0173n/an/a10.1002/advs.201902477Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman SpectroscopyTaek Lee0Mohsen Mohammadniaei1Hui Zhang2Jinho Yoon3Hye Kyu Choi4Sijin Guo5Peixuan Guo6Jeong‐Woo Choi7College of Pharmacy College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute Ohio State University Columbus OH 43210 USADepartment of Chemical and Biomolecular Engineering Sogang University 35 Baekbeom‐ro, Mapo‐gu Seoul 121‐742 Republic of KoreaCollege of Pharmacy College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute Ohio State University Columbus OH 43210 USADepartment of Chemical and Biomolecular Engineering Sogang University 35 Baekbeom‐ro, Mapo‐gu Seoul 121‐742 Republic of KoreaDepartment of Chemical and Biomolecular Engineering Sogang University 35 Baekbeom‐ro, Mapo‐gu Seoul 121‐742 Republic of KoreaCollege of Pharmacy College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute Ohio State University Columbus OH 43210 USACollege of Pharmacy College of Medicine/Department of Physiology and Cell Biology/Dorothy M. Davis Heart and Lung Research Institute Ohio State University Columbus OH 43210 USADepartment of Chemical and Biomolecular Engineering Sogang University 35 Baekbeom‐ro, Mapo‐gu Seoul 121‐742 Republic of KoreaAbstract Controlling the selective one‐to‐one conjugation of RNA with nanoparticles is vital for future applications of RNA nanotechnology. Here, the monofunctionalization of a gold nanoparticle (AuNP) with a single copy of RNA is developed for ultrasensitive microRNA‐155 quantification using electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS). A single AuNP is conjugated with one copy of the packaging RNA (pRNA) three‐way junction (RNA 3WJ). pRNA 3WJ containing one strand of the 3WJ is connected to a Sephadex G100 aptamer and a biotin group at each arm (SEPapt/3WJ/Bio) which is then immobilized to the Sephadex G100 resin. The resulting complex is connected to streptavidin‐coated AuNP (STV/AuNP). Next, the STV/AuNP–Bio/3WJa is purified and reassembled with another 3WJ to form a single‐labeled 3WJ/AuNP. Later, the monoconjugate is immobilized onto the AuNP‐electrodeposited indium tin oxide coated substrate for detecting microRNA‐155 based on EC‐SERS. Application of an optimum potential of +0.2 V results in extraordinary amplification (≈7 times) of methylene blue (reporter) SERS signal compared to the normal SERS signal. As a result, a highly sensitive detection of 60 × 10−18 m microRNA‐155 in 1 h in serum based on monoconjugated AuNP/RNA is achieved. Thus, the monofunctionalization of RNA onto nanoparticle can provide a new methodology for biosensor construction and diverse RNA nanotechnology development.https://doi.org/10.1002/advs.201902477biosensorselectrochemical surface‐enhanced Raman spectroscopy (EC‐SERS)microRNAsmonofunctionalizationpRNA 3WJ |
| spellingShingle | Taek Lee Mohsen Mohammadniaei Hui Zhang Jinho Yoon Hye Kyu Choi Sijin Guo Peixuan Guo Jeong‐Woo Choi Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy Advanced Science biosensors electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS) microRNAs monofunctionalization pRNA 3WJ |
| title | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
| title_full | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
| title_fullStr | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
| title_full_unstemmed | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
| title_short | Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface‐Enhanced Raman Spectroscopy |
| title_sort | single functionalized prna gold nanoparticle for ultrasensitive microrna detection using electrochemical surface enhanced raman spectroscopy |
| topic | biosensors electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS) microRNAs monofunctionalization pRNA 3WJ |
| url | https://doi.org/10.1002/advs.201902477 |
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