The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation.
Influenza A virus (IAV) has evolved various strategies to counteract the innate immune response using different viral proteins. However, the mechanism is not fully elucidated. In this study, we identified the PB1 protein of H7N9 virus as a new negative regulator of virus- or poly(I:C)-stimulated IFN...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-02-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1009300 |
| _version_ | 1818588833990574080 |
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| author | Yan Zeng Shuai Xu Yanli Wei Xuegang Zhang Qian Wang Yane Jia Wanbing Wang Lu Han Zhaoshan Chen Zhengxiang Wang Bo Zhang Hualan Chen Cao-Qi Lei Qiyun Zhu |
| author_facet | Yan Zeng Shuai Xu Yanli Wei Xuegang Zhang Qian Wang Yane Jia Wanbing Wang Lu Han Zhaoshan Chen Zhengxiang Wang Bo Zhang Hualan Chen Cao-Qi Lei Qiyun Zhu |
| author_sort | Yan Zeng |
| collection | DOAJ |
| description | Influenza A virus (IAV) has evolved various strategies to counteract the innate immune response using different viral proteins. However, the mechanism is not fully elucidated. In this study, we identified the PB1 protein of H7N9 virus as a new negative regulator of virus- or poly(I:C)-stimulated IFN induction and specifically interacted with and destabilized MAVS. A subsequent study revealed that PB1 promoted E3 ligase RNF5 to catalyze K27-linked polyubiquitination of MAVS at Lys362 and Lys461. Moreover, we found that PB1 preferentially associated with a selective autophagic receptor neighbor of BRCA1 (NBR1) that recognizes ubiquitinated MAVS and delivers it to autophagosomes for degradation. The degradation cascade mediated by PB1 facilitates H7N9 virus infection by blocking the RIG-I-MAVS-mediated innate signaling pathway. Taken together, these data uncover a negative regulatory mechanism involving the PB1-RNF5-MAVS-NBR1 axis and provide insights into an evasion strategy employed by influenza virus that involves selective autophagy and innate signaling pathways. |
| first_indexed | 2024-12-16T09:31:03Z |
| format | Article |
| id | doaj.art-538291fff6874762bc9ab34c73220efb |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-16T09:31:03Z |
| publishDate | 2021-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-538291fff6874762bc9ab34c73220efb2022-12-21T22:36:32ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-02-01172e100930010.1371/journal.ppat.1009300The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation.Yan ZengShuai XuYanli WeiXuegang ZhangQian WangYane JiaWanbing WangLu HanZhaoshan ChenZhengxiang WangBo ZhangHualan ChenCao-Qi LeiQiyun ZhuInfluenza A virus (IAV) has evolved various strategies to counteract the innate immune response using different viral proteins. However, the mechanism is not fully elucidated. In this study, we identified the PB1 protein of H7N9 virus as a new negative regulator of virus- or poly(I:C)-stimulated IFN induction and specifically interacted with and destabilized MAVS. A subsequent study revealed that PB1 promoted E3 ligase RNF5 to catalyze K27-linked polyubiquitination of MAVS at Lys362 and Lys461. Moreover, we found that PB1 preferentially associated with a selective autophagic receptor neighbor of BRCA1 (NBR1) that recognizes ubiquitinated MAVS and delivers it to autophagosomes for degradation. The degradation cascade mediated by PB1 facilitates H7N9 virus infection by blocking the RIG-I-MAVS-mediated innate signaling pathway. Taken together, these data uncover a negative regulatory mechanism involving the PB1-RNF5-MAVS-NBR1 axis and provide insights into an evasion strategy employed by influenza virus that involves selective autophagy and innate signaling pathways.https://doi.org/10.1371/journal.ppat.1009300 |
| spellingShingle | Yan Zeng Shuai Xu Yanli Wei Xuegang Zhang Qian Wang Yane Jia Wanbing Wang Lu Han Zhaoshan Chen Zhengxiang Wang Bo Zhang Hualan Chen Cao-Qi Lei Qiyun Zhu The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. PLoS Pathogens |
| title | The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. |
| title_full | The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. |
| title_fullStr | The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. |
| title_full_unstemmed | The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. |
| title_short | The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation. |
| title_sort | pb1 protein of influenza a virus inhibits the innate immune response by targeting mavs for nbr1 mediated selective autophagic degradation |
| url | https://doi.org/10.1371/journal.ppat.1009300 |
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