Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques
Abstract Narrow band-ultraviolet B (NB-UVB) is an effective treatment for psoriasis. We aim to generate a potential mechanism of NB-UVB through comparing the transcriptomic profile before and after NB-UVB treatment between the peripheral edge of lesional skin (PE skin) and the center of lesional ski...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31610-y |
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| author | Suphagan Boonpethkaew Jitlada Meephansan Sasin Charoensuksira Onjira Jumlongpim Pattarin Tangtanatakul Jongkonnee Wongpiyabovorn Mayumi Komine Akimichi Morita |
| author_facet | Suphagan Boonpethkaew Jitlada Meephansan Sasin Charoensuksira Onjira Jumlongpim Pattarin Tangtanatakul Jongkonnee Wongpiyabovorn Mayumi Komine Akimichi Morita |
| author_sort | Suphagan Boonpethkaew |
| collection | DOAJ |
| description | Abstract Narrow band-ultraviolet B (NB-UVB) is an effective treatment for psoriasis. We aim to generate a potential mechanism of NB-UVB through comparing the transcriptomic profile before and after NB-UVB treatment between the peripheral edge of lesional skin (PE skin) and the center of lesional skin (CE skin) on the basis of molecular mechanisms of these two areas display different downstream functions. More than one-fourth of the NB-UVB-altered genes were found to be plaque-specific. Some of them were psoriasis signature genes that were downregulated by NB-UVB in, both, PE and CE skin (core alteration), such as IL36G, DEFB4A/B, S100A15, KRT16, and KRT6A. After NB-UVB treatment, the activity score of upstream cytokines, such as interferons, interleukin (IL)-6, IL-17, and IL-22 in pathogenesis decreased. In addition, NB-UVB could restore normal keratinization by upregulating LORICRIN and KRT2, particularly in the CE skin. Finally, we illustrated that NB-UVB is capable of suppressing molecules from the initiation to maintenance phase of plaque formation, thereby normalizing psoriatic plaques. This finding supports the usefulness of NB-UVB treatment in clinical practice and may help in the development of new treatment approaches in which NB-UVB treatment is included for patients with psoriasis or other inflammatory skin diseases. |
| first_indexed | 2024-03-10T21:59:17Z |
| format | Article |
| id | doaj.art-5387cdbf10aa4deaa0bd207b3fdc6313 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:59:17Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5387cdbf10aa4deaa0bd207b3fdc63132023-11-19T13:00:23ZengNature PortfolioScientific Reports2045-23222023-03-0113111110.1038/s41598-023-31610-yElucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaquesSuphagan Boonpethkaew0Jitlada Meephansan1Sasin Charoensuksira2Onjira Jumlongpim3Pattarin Tangtanatakul4Jongkonnee Wongpiyabovorn5Mayumi Komine6Akimichi Morita7Division of Dermatology, Chulabhorn International College of Medicine, Thammasat UniversityDivision of Dermatology, Chulabhorn International College of Medicine, Thammasat UniversityDivision of Dermatology, Chulabhorn International College of Medicine, Thammasat UniversityDivision of Dermatology, Chulabhorn International College of Medicine, Thammasat UniversityDepartment of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn UniversityDepartment of Microbiology, Faculty of Medicine, Center of Excellence in Immunology and Immune-Mediated Disease, Chulalongkorn UniversityDepartment of Dermatology, Jichi Medical UniversityDepartment of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical SciencesAbstract Narrow band-ultraviolet B (NB-UVB) is an effective treatment for psoriasis. We aim to generate a potential mechanism of NB-UVB through comparing the transcriptomic profile before and after NB-UVB treatment between the peripheral edge of lesional skin (PE skin) and the center of lesional skin (CE skin) on the basis of molecular mechanisms of these two areas display different downstream functions. More than one-fourth of the NB-UVB-altered genes were found to be plaque-specific. Some of them were psoriasis signature genes that were downregulated by NB-UVB in, both, PE and CE skin (core alteration), such as IL36G, DEFB4A/B, S100A15, KRT16, and KRT6A. After NB-UVB treatment, the activity score of upstream cytokines, such as interferons, interleukin (IL)-6, IL-17, and IL-22 in pathogenesis decreased. In addition, NB-UVB could restore normal keratinization by upregulating LORICRIN and KRT2, particularly in the CE skin. Finally, we illustrated that NB-UVB is capable of suppressing molecules from the initiation to maintenance phase of plaque formation, thereby normalizing psoriatic plaques. This finding supports the usefulness of NB-UVB treatment in clinical practice and may help in the development of new treatment approaches in which NB-UVB treatment is included for patients with psoriasis or other inflammatory skin diseases.https://doi.org/10.1038/s41598-023-31610-y |
| spellingShingle | Suphagan Boonpethkaew Jitlada Meephansan Sasin Charoensuksira Onjira Jumlongpim Pattarin Tangtanatakul Jongkonnee Wongpiyabovorn Mayumi Komine Akimichi Morita Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques Scientific Reports |
| title | Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| title_full | Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| title_fullStr | Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| title_full_unstemmed | Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| title_short | Elucidating the NB-UVB mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| title_sort | elucidating the nb uvb mechanism by comparing transcriptome alteration on the edge and center of psoriatic plaques |
| url | https://doi.org/10.1038/s41598-023-31610-y |
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