The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation
Objective: Assessments of cell reactions such as motility, orientation and activation to the topography of the substratum will assist with the fabrication of a proper implantable scaffold for future tissue engineering applications.The current challenge is to analyze the orientation effect of elec...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2014-04-01
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| Series: | Cell Journal |
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| Online Access: | http://celljournal.org/library/upload/article/af_23344247324852436622352347222252525244452-Naghmeh%20Abbasi-email.pdf |
| _version_ | 1818273546573447168 |
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| author | Naghmeh Abbasi Sara Soudi, Nasim Hayati-Roodbari Masumeh Dodel Masoud Soleimani |
| author_facet | Naghmeh Abbasi Sara Soudi, Nasim Hayati-Roodbari Masumeh Dodel Masoud Soleimani |
| author_sort | Naghmeh Abbasi |
| collection | DOAJ |
| description | Objective: Assessments of cell reactions such as motility, orientation and activation to
the topography of the substratum will assist with the fabrication of a proper implantable
scaffold for future tissue engineering applications.The current challenge is to analyze the
orientation effect of elecrospun nanofibers of poly (ε-caprolactone) (PCL) on viability and
proliferation of mouse embryonic stem cells (mESCs).
Materials and Methods: In this experimental study, we used the electrospinning method
to fabricate nanofibrous PCL scaffolds. Chemical and mechanical characterizations were
specified by the contact angle and tensile test. O2 plasma treatment was used to improve
surface hydrophilicity. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay to evaluate mESCs adhesion and proliferation before and after surface
modification. The influence of the orientation of the nanofibers on mESCs growth was
evaluated by scanning electron microscopy (SEM). Statistical analysis was performed
using one-way analysis of variance (ANOVA) With differences considered statistically significant
at p≤0.05.
Results: The results showed that plasma treatment improved the hydrophilic property
of PCL scaffolds. MTT assay showed a significant increase in proliferation of mESCs on
plasma treated PCL (p-PCL) scaffolds compared to non-treated PCL (p≤0.05). However
gelatin coated tissue culture plate (TCP) had a better effect in initial cell attachment after
one day of cell seeding. There was more cell proliferation on day 3 in aligned plasma
treated (AP) nanofibers compared to the TCP. SEM showed optical density of the cell
colonies. Aligned nanofibrous scaffolds had larger colony sizes and spread more than
random nanofibrous scaffolds.
Conclusion: This study showed that plasma treating of scaffolds was a more suitable
substrate for growth and cell attachment. In addition, aligned nanofibrous scaffolds highly
supported the proliferation and spreading of mESCs when compared to random nanofibrous
scaffolds and TCP. |
| first_indexed | 2024-12-12T21:59:41Z |
| format | Article |
| id | doaj.art-538d4db1cd454f90a88121304643ee5c |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-12T21:59:41Z |
| publishDate | 2014-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-538d4db1cd454f90a88121304643ee5c2022-12-22T00:10:33ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142014-04-01163245254The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell ProliferationNaghmeh Abbasi0Sara Soudi,1Nasim Hayati-Roodbari2Masumeh Dodel3Masoud Soleimani4Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran and Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, IranDepartment of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, IranDepartment of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran and Department of Textile Engineering, Amirkabir University of Technology, Tehran, IranDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran and Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, IranObjective: Assessments of cell reactions such as motility, orientation and activation to the topography of the substratum will assist with the fabrication of a proper implantable scaffold for future tissue engineering applications.The current challenge is to analyze the orientation effect of elecrospun nanofibers of poly (ε-caprolactone) (PCL) on viability and proliferation of mouse embryonic stem cells (mESCs). Materials and Methods: In this experimental study, we used the electrospinning method to fabricate nanofibrous PCL scaffolds. Chemical and mechanical characterizations were specified by the contact angle and tensile test. O2 plasma treatment was used to improve surface hydrophilicity. We used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate mESCs adhesion and proliferation before and after surface modification. The influence of the orientation of the nanofibers on mESCs growth was evaluated by scanning electron microscopy (SEM). Statistical analysis was performed using one-way analysis of variance (ANOVA) With differences considered statistically significant at p≤0.05. Results: The results showed that plasma treatment improved the hydrophilic property of PCL scaffolds. MTT assay showed a significant increase in proliferation of mESCs on plasma treated PCL (p-PCL) scaffolds compared to non-treated PCL (p≤0.05). However gelatin coated tissue culture plate (TCP) had a better effect in initial cell attachment after one day of cell seeding. There was more cell proliferation on day 3 in aligned plasma treated (AP) nanofibers compared to the TCP. SEM showed optical density of the cell colonies. Aligned nanofibrous scaffolds had larger colony sizes and spread more than random nanofibrous scaffolds. Conclusion: This study showed that plasma treating of scaffolds was a more suitable substrate for growth and cell attachment. In addition, aligned nanofibrous scaffolds highly supported the proliferation and spreading of mESCs when compared to random nanofibrous scaffolds and TCP.http://celljournal.org/library/upload/article/af_23344247324852436622352347222252525244452-Naghmeh%20Abbasi-email.pdfEmbryonic Stem CellsNanofibersPoly (ε-caprolactone)Surface ModificationCell Proliferation |
| spellingShingle | Naghmeh Abbasi Sara Soudi, Nasim Hayati-Roodbari Masumeh Dodel Masoud Soleimani The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation Cell Journal Embryonic Stem Cells Nanofibers Poly (ε-caprolactone) Surface Modification Cell Proliferation |
| title | The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation |
| title_full | The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation |
| title_fullStr | The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation |
| title_full_unstemmed | The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation |
| title_short | The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation |
| title_sort | effects of plasma treated electrospun nanofibrous poly ε caprolactone scaffolds with different orientations on mouse embryonic stem cell proliferation |
| topic | Embryonic Stem Cells Nanofibers Poly (ε-caprolactone) Surface Modification Cell Proliferation |
| url | http://celljournal.org/library/upload/article/af_23344247324852436622352347222252525244452-Naghmeh%20Abbasi-email.pdf |
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