Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS)
For the purpose of human disease prevention, several methods have been developed, and are still developing, to assess the oxidative stress status (OSS) of individuals. In the present paper, we describe an approach based on electrochemical detection able to evaluate skin oxidative stress status (SOSS...
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MDPI AG
2019-05-01
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| Series: | Diseases |
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| Online Access: | https://www.mdpi.com/2079-9721/7/2/40 |
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| author | Pincemail Joël Kaci Mouna-Messaouda Cheramy-Bien Jean-Paul Defraigne Jean-Olivier Meziane Smail |
| author_facet | Pincemail Joël Kaci Mouna-Messaouda Cheramy-Bien Jean-Paul Defraigne Jean-Olivier Meziane Smail |
| author_sort | Pincemail Joël |
| collection | DOAJ |
| description | For the purpose of human disease prevention, several methods have been developed, and are still developing, to assess the oxidative stress status (OSS) of individuals. In the present paper, we describe an approach based on electrochemical detection able to evaluate skin oxidative stress status (SOSS) as a PAOT (Pouvoir AntiOxydant Total)-Skin Score<sup>®</sup>. Normal reference values for the PAOT-Skin Score<sup>®</sup> were: 0−62.94 (<i>n</i> = 263). Intra- and inter-assay coefficients of variation were, respectively, 12.47 ± 4.29% and 7.0 ± 2.5%. Our technology showed increased skin antioxidant activity following topical application of reduced coeznyme Q<sub>10</sub> cream or vitamin C intake as orange juice or supplements. Moreover, we found significant correlations between some blood oxidative stress biomarkers and the PAOT-Skin Score<sup>®</sup> (γ-tocopherol/α-tocopherol ratio (r = 0.43, <i>p</i> = 0.020); copper (r = −0.42, <i>p</i> = 0.022); copper/zinc ratio (r = −0.49, <i>p</i> = 0.006), and lipid peroxides (r = −0.43, <i>p</i> = 0.002)). In addition to being non−invasive, the present electrochemical methodology is also not expensive, fast, and easy to use. |
| first_indexed | 2024-04-12T01:49:18Z |
| format | Article |
| id | doaj.art-53931ee9160e4c639cdd2080f02fb469 |
| institution | Directory Open Access Journal |
| issn | 2079-9721 |
| language | English |
| last_indexed | 2024-04-12T01:49:18Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diseases |
| spelling | doaj.art-53931ee9160e4c639cdd2080f02fb4692022-12-22T03:52:58ZengMDPI AGDiseases2079-97212019-05-01724010.3390/diseases7020040diseases7020040Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS)Pincemail Joël0Kaci Mouna-Messaouda1Cheramy-Bien Jean-Paul2Defraigne Jean-Olivier3Meziane Smail4Department of Cardiovascular Surgery/CREDEC, Antioxidant Nutrition and Health Platform, University of Liège and CHU, Sart Tilman, 4000 Liège, BelgiumInstitut Européen des Antioxydants, Oxystress Technologies, 1 rue Victor de Lespinats, 54230 Neuves-Maisons, FranceDepartment of Cardiovascular Surgery/CREDEC, Antioxidant Nutrition and Health Platform, University of Liège and CHU, Sart Tilman, 4000 Liège, BelgiumDepartment of Cardiovascular Surgery/CREDEC, Antioxidant Nutrition and Health Platform, University of Liège and CHU, Sart Tilman, 4000 Liège, BelgiumInstitut Européen des Antioxydants, Oxystress Technologies, 1 rue Victor de Lespinats, 54230 Neuves-Maisons, FranceFor the purpose of human disease prevention, several methods have been developed, and are still developing, to assess the oxidative stress status (OSS) of individuals. In the present paper, we describe an approach based on electrochemical detection able to evaluate skin oxidative stress status (SOSS) as a PAOT (Pouvoir AntiOxydant Total)-Skin Score<sup>®</sup>. Normal reference values for the PAOT-Skin Score<sup>®</sup> were: 0−62.94 (<i>n</i> = 263). Intra- and inter-assay coefficients of variation were, respectively, 12.47 ± 4.29% and 7.0 ± 2.5%. Our technology showed increased skin antioxidant activity following topical application of reduced coeznyme Q<sub>10</sub> cream or vitamin C intake as orange juice or supplements. Moreover, we found significant correlations between some blood oxidative stress biomarkers and the PAOT-Skin Score<sup>®</sup> (γ-tocopherol/α-tocopherol ratio (r = 0.43, <i>p</i> = 0.020); copper (r = −0.42, <i>p</i> = 0.022); copper/zinc ratio (r = −0.49, <i>p</i> = 0.006), and lipid peroxides (r = −0.43, <i>p</i> = 0.002)). In addition to being non−invasive, the present electrochemical methodology is also not expensive, fast, and easy to use.https://www.mdpi.com/2079-9721/7/2/40oxidative stressskinelectrochemical detectionPAOT-Skin Score<sup>®</sup> |
| spellingShingle | Pincemail Joël Kaci Mouna-Messaouda Cheramy-Bien Jean-Paul Defraigne Jean-Olivier Meziane Smail Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) Diseases oxidative stress skin electrochemical detection PAOT-Skin Score<sup>®</sup> |
| title | Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) |
| title_full | Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) |
| title_fullStr | Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) |
| title_full_unstemmed | Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) |
| title_short | Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS) |
| title_sort | electrochemical methodology for evaluating skin oxidative stress status soss |
| topic | oxidative stress skin electrochemical detection PAOT-Skin Score<sup>®</sup> |
| url | https://www.mdpi.com/2079-9721/7/2/40 |
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