Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep
Abstract In this study, we used 10 healthy sheep, which gave birth to healthy twins. Stool samples were collected from mothers and their offspring 3 times during the study (0, 28 and 56 day postpartum). Milk samples were taken from the mothers at the same time. RT PCR analysis of faeces and milk was...
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| Format: | Article |
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Nature Portfolio
2021-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-03207-w |
| _version_ | 1818344146268585984 |
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| author | Paulina Cholewińska Magdalena Wołoszyńska Marta Michalak Katarzyna Czyż Witold Rant Jakub Smoliński Anna Wyrostek Konrad Wojnarowski |
| author_facet | Paulina Cholewińska Magdalena Wołoszyńska Marta Michalak Katarzyna Czyż Witold Rant Jakub Smoliński Anna Wyrostek Konrad Wojnarowski |
| author_sort | Paulina Cholewińska |
| collection | DOAJ |
| description | Abstract In this study, we used 10 healthy sheep, which gave birth to healthy twins. Stool samples were collected from mothers and their offspring 3 times during the study (0, 28 and 56 day postpartum). Milk samples were taken from the mothers at the same time. RT PCR analysis of faeces and milk was performed in order to assess the level of bacteria from the Firmicutes and Bacteroidetes phyla including the family Lactobacillaceae (phylum Firmicutes). The composition of mother's milk was also analyzed and their BCS. The data were compiled statistically. The obtained results showed that the level of the studied groups of bacteria may change due to the change of diet. Additionally, there were significant differences between lambs and mothers in the levels of the studied groups of bacteria. Analysis also shown that in the digestive system of mothers was a smaller disproportion in the level of the studied bacterial phyla than in lambs. The results also indicated the occurrence of differences in the bacterial composition at the individual level, both in ewes and their offspring. Additionally, in the conducted experiment, there were differences in the level of Firmicutes and Bacteroidetes groups depending on the sex. |
| first_indexed | 2024-12-13T16:41:50Z |
| format | Article |
| id | doaj.art-5394c6f814584a208cc5a79d32cede59 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:41:50Z |
| publishDate | 2021-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5394c6f814584a208cc5a79d32cede592022-12-21T23:38:15ZengNature PortfolioScientific Reports2045-23222021-12-0111111810.1038/s41598-021-03207-wInfluence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheepPaulina Cholewińska0Magdalena Wołoszyńska1Marta Michalak2Katarzyna Czyż3Witold Rant4Jakub Smoliński5Anna Wyrostek6Konrad Wojnarowski7Institute of Animal Breeding, Wroclaw University of Environmental and Life SciencesDepartment of Genetics, Wroclaw University of Environmental and Life SciencesDepartment of Animal Nutrition and Feed Management, Wroclaw University of Environmental and Life SciencesInstitute of Animal Breeding, Wroclaw University of Environmental and Life SciencesInstitute of Animal Breeding, Warsaw University of Life Sciences – SGGWInstitute of Animal Breeding, Wroclaw University of Environmental and Life SciencesInstitute of Animal Breeding, Wroclaw University of Environmental and Life SciencesInstitute of Animal Breeding, Wroclaw University of Environmental and Life SciencesAbstract In this study, we used 10 healthy sheep, which gave birth to healthy twins. Stool samples were collected from mothers and their offspring 3 times during the study (0, 28 and 56 day postpartum). Milk samples were taken from the mothers at the same time. RT PCR analysis of faeces and milk was performed in order to assess the level of bacteria from the Firmicutes and Bacteroidetes phyla including the family Lactobacillaceae (phylum Firmicutes). The composition of mother's milk was also analyzed and their BCS. The data were compiled statistically. The obtained results showed that the level of the studied groups of bacteria may change due to the change of diet. Additionally, there were significant differences between lambs and mothers in the levels of the studied groups of bacteria. Analysis also shown that in the digestive system of mothers was a smaller disproportion in the level of the studied bacterial phyla than in lambs. The results also indicated the occurrence of differences in the bacterial composition at the individual level, both in ewes and their offspring. Additionally, in the conducted experiment, there were differences in the level of Firmicutes and Bacteroidetes groups depending on the sex.https://doi.org/10.1038/s41598-021-03207-w |
| spellingShingle | Paulina Cholewińska Magdalena Wołoszyńska Marta Michalak Katarzyna Czyż Witold Rant Jakub Smoliński Anna Wyrostek Konrad Wojnarowski Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep Scientific Reports |
| title | Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep |
| title_full | Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep |
| title_fullStr | Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep |
| title_full_unstemmed | Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep |
| title_short | Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep |
| title_sort | influence of selected factors on the firmicutes bacteroidetes phyla and the lactobacillaceae family in the digestive tract of sheep |
| url | https://doi.org/10.1038/s41598-021-03207-w |
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