Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids
A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composit...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/10/1990 |
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| author | Juraj Vodicka Monika Wikarska Monika Trudicova Zuzana Juglova Aneta Pospisilova Michal Kalina Eva Slaninova Stanislav Obruca Petr Sedlacek |
| author_facet | Juraj Vodicka Monika Wikarska Monika Trudicova Zuzana Juglova Aneta Pospisilova Michal Kalina Eva Slaninova Stanislav Obruca Petr Sedlacek |
| author_sort | Juraj Vodicka |
| collection | DOAJ |
| description | A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate—P(3HB-<i>co</i>-36 mol.% 4HB) and P(3HB-<i>co</i>-66 mol.% 4HB), by cultivating the thermophilic bacterial strain <i>Aneurinibacillus</i> sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-<i>co</i>-4HB) copolymers produced by <i>Aneurinibacillus</i> sp. H1 as being very auspicious polymers for intestinal in vivo treatments. |
| first_indexed | 2024-03-10T03:01:41Z |
| format | Article |
| id | doaj.art-539773edb9794c439e2a5ae0d6307fbd |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:01:41Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-539773edb9794c439e2a5ae0d6307fbd2023-11-23T12:45:43ZengMDPI AGPolymers2073-43602022-05-011410199010.3390/polym14101990Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body FluidsJuraj Vodicka0Monika Wikarska1Monika Trudicova2Zuzana Juglova3Aneta Pospisilova4Michal Kalina5Eva Slaninova6Stanislav Obruca7Petr Sedlacek8Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech RepublicA novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate—P(3HB-<i>co</i>-36 mol.% 4HB) and P(3HB-<i>co</i>-66 mol.% 4HB), by cultivating the thermophilic bacterial strain <i>Aneurinibacillus</i> sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-<i>co</i>-4HB) copolymers produced by <i>Aneurinibacillus</i> sp. H1 as being very auspicious polymers for intestinal in vivo treatments.https://www.mdpi.com/2073-4360/14/10/1990polyhydroxyalkanoates (PHA)<i>Aneurinibacillus</i> sp. H1PHA copolymersbiodegradationsimulated body fluids |
| spellingShingle | Juraj Vodicka Monika Wikarska Monika Trudicova Zuzana Juglova Aneta Pospisilova Michal Kalina Eva Slaninova Stanislav Obruca Petr Sedlacek Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids Polymers polyhydroxyalkanoates (PHA) <i>Aneurinibacillus</i> sp. H1 PHA copolymers biodegradation simulated body fluids |
| title | Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids |
| title_full | Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids |
| title_fullStr | Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids |
| title_full_unstemmed | Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids |
| title_short | Degradation of P(3HB-<i>co</i>-4HB) Films in Simulated Body Fluids |
| title_sort | degradation of p 3hb i co i 4hb films in simulated body fluids |
| topic | polyhydroxyalkanoates (PHA) <i>Aneurinibacillus</i> sp. H1 PHA copolymers biodegradation simulated body fluids |
| url | https://www.mdpi.com/2073-4360/14/10/1990 |
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