RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment
Abstract Avicennia marina (Forsk.) Vierh. is a typical mangrove plant. Its epidermis contains salt glands, which can secrete excess salts onto the leaf surfaces, improving the salt tolerance of the plants. However, knowledge on the epidermis-specific transcriptional responses of A. marina to salinit...
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34095-x |
| _version_ | 1797827568426024960 |
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| author | Huan Li Chao-Tian Lv Yun-Tao Li Guo-Yv Gao Ya-Fei Meng Yv-Le You Qi Tian Kun-Qi Liang Yu Chen Hao Chen Chao Xia Xiang-Yun Rui Hai-Lei Zheng Ming-Yue Wei |
| author_facet | Huan Li Chao-Tian Lv Yun-Tao Li Guo-Yv Gao Ya-Fei Meng Yv-Le You Qi Tian Kun-Qi Liang Yu Chen Hao Chen Chao Xia Xiang-Yun Rui Hai-Lei Zheng Ming-Yue Wei |
| author_sort | Huan Li |
| collection | DOAJ |
| description | Abstract Avicennia marina (Forsk.) Vierh. is a typical mangrove plant. Its epidermis contains salt glands, which can secrete excess salts onto the leaf surfaces, improving the salt tolerance of the plants. However, knowledge on the epidermis-specific transcriptional responses of A. marina to salinity treatment is lacking. Thus, physiological and transcriptomic techniques were applied to unravel the salt tolerance mechanism of A. marina. Our results showed that 400 mM NaCl significantly reduced the plant height, leaf area, leaf biomass and photosynthesis of A. marina. In addition, 1565 differentially expressed genes were identified, of which 634 and 931 were up- and down-regulated. Based on Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis, we demonstrated that decreased gene expression, especially that of OEE1, PQL2, FDX3, ATPC, GAPDH, PRK, FBP and RPE, could explain the inhibited photosynthesis caused by salt treatment. Furthermore, the ability of A. marina to cope with 400 mM NaCl treatment was dependent on appropriate hormone signalling and potential sulfur-containing metabolites, such as hydrogen sulfide and cysteine biosynthesis. Overall, the present study provides a theoretical basis for the adaption of A. marina to saline habitats and a reference for studying the salt tolerance mechanism of other mangrove plants. |
| first_indexed | 2024-04-09T12:50:25Z |
| format | Article |
| id | doaj.art-539aa401d6934dcdb5a1189db38225f6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T12:50:25Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-539aa401d6934dcdb5a1189db38225f62023-05-14T11:13:33ZengNature PortfolioScientific Reports2045-23222023-05-0113111110.1038/s41598-023-34095-xRNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatmentHuan Li0Chao-Tian Lv1Yun-Tao Li2Guo-Yv Gao3Ya-Fei Meng4Yv-Le You5Qi Tian6Kun-Qi Liang7Yu Chen8Hao Chen9Chao Xia10Xiang-Yun Rui11Hai-Lei Zheng12Ming-Yue Wei13College of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityCollege of Food and Bio-Engineering, Bengbu UniversityKey Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen UniversitySchool of Ecology, Resources and Environment, Dezhou UniversityAbstract Avicennia marina (Forsk.) Vierh. is a typical mangrove plant. Its epidermis contains salt glands, which can secrete excess salts onto the leaf surfaces, improving the salt tolerance of the plants. However, knowledge on the epidermis-specific transcriptional responses of A. marina to salinity treatment is lacking. Thus, physiological and transcriptomic techniques were applied to unravel the salt tolerance mechanism of A. marina. Our results showed that 400 mM NaCl significantly reduced the plant height, leaf area, leaf biomass and photosynthesis of A. marina. In addition, 1565 differentially expressed genes were identified, of which 634 and 931 were up- and down-regulated. Based on Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis, we demonstrated that decreased gene expression, especially that of OEE1, PQL2, FDX3, ATPC, GAPDH, PRK, FBP and RPE, could explain the inhibited photosynthesis caused by salt treatment. Furthermore, the ability of A. marina to cope with 400 mM NaCl treatment was dependent on appropriate hormone signalling and potential sulfur-containing metabolites, such as hydrogen sulfide and cysteine biosynthesis. Overall, the present study provides a theoretical basis for the adaption of A. marina to saline habitats and a reference for studying the salt tolerance mechanism of other mangrove plants.https://doi.org/10.1038/s41598-023-34095-x |
| spellingShingle | Huan Li Chao-Tian Lv Yun-Tao Li Guo-Yv Gao Ya-Fei Meng Yv-Le You Qi Tian Kun-Qi Liang Yu Chen Hao Chen Chao Xia Xiang-Yun Rui Hai-Lei Zheng Ming-Yue Wei RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment Scientific Reports |
| title | RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment |
| title_full | RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment |
| title_fullStr | RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment |
| title_full_unstemmed | RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment |
| title_short | RNA-sequencing transcriptome analysis of Avicennia marina (Forsk.) Vierh. leaf epidermis defines tissue-specific transcriptional response to salinity treatment |
| title_sort | rna sequencing transcriptome analysis of avicennia marina forsk vierh leaf epidermis defines tissue specific transcriptional response to salinity treatment |
| url | https://doi.org/10.1038/s41598-023-34095-x |
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